Oligosaccharides from Botrytis cinerea and Elicitation of Grapevine Defense

Saigne-Soulard, Cassandrine; Abdelli-Belhadj, Assia; Télef-Micouleau, Marie; Bouscaut, Jérôme; Cluzet, Stéphanie; Corio‐Costet, Marie‐France; Mérillon, Jean‐Michel

doi:10.1007/978-3-319-16298-0_8

Cited by 11 publications

(6 citation statements)

References 107 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several experiments with grapevine cell cultures have shown that cells synthesize and accumulate resveratrol in the extracellular medium, in response to elicitors. − By contrast, piceid and ε-viniferin generally accumulate in higher concentration inside the cells. , In the present work, for SE condition, only the stilbenes contained in the cane extract, that is, resveratrol, ε-viniferin, and piceatannol (but not piceid nor δ viniferin) were detected in the culture medium or inside cells. Several reports have previously shown that after treatment with elicitors there is an accumulation of stilbenes in the first 48 h, ,, similar to what we observed in response to OG. However, no accumulation in culture medium could be detected in response to SE during this period since the highest levels were detected just after SE addition to cell suspensions.…”

Section: Resultssupporting

confidence: 90%

“…Grapevine cell cultures respond to elicitors by synthesizing and accumulating stilbenoid phytoalexins, mainly resveratrol and its dimer viniferin . The potential release of resveratrol into the culture medium after SE-treatment was first monitored and compared with that observed after treatment with oligogalacturonides (OG), a well-known elicitor of grapevine defense. , (Figure A, Figure S4). Very high resveratrol concentrations were detected immediately after the addition of SE (510 μg/g FW), followed by a progressive decrease until 24 h (16.69 μg/g FW).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Dual Mode of Action of Grape Cane Extracts against Botrytis cinerea

Bona

Adrian

Négrel

et al. 2019

J. Agric. Food Chem.

View full text Add to dashboard Cite

Crude extracts of Vitis vinifera canes represent a natural source of stilbene compounds with well characterized antifungals properties. In our trials, exogenous application of a stilbene extract (SE) obtained from grape canes on grapevine leaves reduces the necrotic lesions caused by Botrytis cinerea. The SE showed to possess a direct antifungal activity by inhibiting the mycelium growth. The activation of some grapevine defense mechanism was also investigated. H2O2 production and activation of mitogen-activated protein kinase (MAPK) phosphorylation cascades as well as accumulation of stilbenoid phytoalexins were explored on grapevine cell suspension. Moreover, the transcription of genes encoding for proteins affecting defense responses was analyzed on grapevine plants. The SE induced some grapevine defense mechanisms including MAPK activation, and the expression of pathogenesis-related (PR) genes and of a gene encoding the glutathione-S-transferase 1 (GST1). By contrast, treatment of grapevine leaves with SE negatively regulates de novo stilbene production.

show abstract

Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Dual Mode of Action of Grape Cane Extracts against Botrytis cinerea

Bona

Adrian

Négrel

et al. 2019

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…In leaf samples, only piceid accumulated. The absence of resveratrol was quite unexpected, as it was often reported in grapevine that foliar application of some elicitors, such as oligosaccharides from B. cinerea culture extracts ( Saigne-Soulard et al, 2015 ), chitosan ( Aziz et al, 2006 ) or MeJA ( Belhadj et al, 2006 ) were able to induce piceid, and also resveratrol production. In our conditions, neither resveratrol was detected in untreated-infected samples, whereas authors usually report its accumulation in response to P. viticola infection ( Schmidlin et al, 2008 ).…”

Section: Discussionmentioning

confidence: 95%

A Plant Extract Acts Both as a Resistance Inducer and an Oomycide Against Grapevine Downy Mildew

et al. 2018

Self Cite

View full text Add to dashboard Cite

Protecting vineyards from cryptogamic diseases such as downy mildew, caused by Plasmopara viticola, generally requires a massive use of phytochemicals. However, the issues on unintentional secondary effects on environment and human health, and the occurrence of P. viticola resistant strains, are leading to the development of alternative strategies, such as the use of biocontrol products. In this paper, we evidenced the ability of a plant extract to protect grapevine from P. viticola. Further experiments carried out both on cell suspensions and on plants revealed that plant extract activates typical defense-related responses such as the production of H2O2, the up-regulation of genes encoding pathogenesis-related proteins and stilbene synthase, as well as the accumulation of resveratrol or its derivative piceid. We also brought to light a strong direct effect of PE on the release and motility of P. viticola zoospores. Furthermore, we found out that PE application left dried residues on leaf surface, impairing zoospores to reach stomata. Altogether, our results highlight the different modes of action of a new biocontrol product able to protect grapevine against downy mildew.

show abstract

“…Indeed, many secondary metabolites synthesized by plants to defend themselves against pathogens have antimicrobial properties. The alternative approach is to find elicitor compounds for grapevine defense stimulation, but only a few commercial products with low efficacy have been approved for this purpose (Aziz et al, 2003;Saigne-Soulard et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

<em>Vitis vinifera</em> canes, a source of stilbenoids against downy mildew

et al. 2016

Self Cite

View full text Add to dashboard Cite

Aim:To investigate the antifungal efficacy of grape cane extracts enriched in stilbenes against Plasmopara viticola by in vivo experiments on grape plants. Methods and results:Experiments on grape plants were carried out from 2007 to 2008 at commercial vineyards in « Le Tourne » (southwest region of France) on Vitis vinifera L. cv Merlot plants and in a greenhouse, where 45-day-old plants (Merlot) were used. Grapevine plants were treated with stilbene extract (STE) or « Bordeaux mixture » (BM) as positive control. STE, prepared from Vitis vinifera canes, contained 33% of total stilbenes ranging from monomers to tetramers and was used at 5g L -1 . The degree of protection for downy mildew was determined as the percentage of infected leaf surface area and pathogen attack frequency. On young plants in greenhouse, P. viticola inoculation and three treatments with STE were carried out. After the treatments, the disease reduction was from 59 to 69% for pathogen attack frequency and from 83 to 88% for infected leaf surface for the two modes of infestation (artificial and secondary). STE triggered a similar intense decrease in downy mildew infection as recorded for BM. In the vineyard assay, downy mildew infection was allowed to occur naturally. Field grown plants were treated every 7 th day for eight weeks or every 14 th day for eight weeks during June and July. We observed that STE was also able to prevent the infection of P. viticola in vineyard but to a lesser extent. Treatment with STE every 7 th day provided better results in terms of infection frequency and leaf area than that every 14 th day. STE treatment of plants every 7 th day in vineyard reduced the frequency of attack by 16 to 39% and leaf surface infection by 57 to 61%, which was lower than that recorded for BM. STE also displayed in vitro inhibitory effects on the release of zoospores from sporangia and the germination of zoospores of P. viticola. Zoospore germination was completely inhibited at a concentration of 100 mg L -1 and zoospore release was inhibited at 330 mg L -1 . Conclusions:Treatment of young grapevine plants in greenhouse with stilbene extract from grapevine canes offered considerable protection against P. viticola that was identical to that provided by Bordeaux mixture. STE was also able to prevent the infection of P. viticola in vineyard but to a lesser extent. Treatment with STE every 7 th day in vineyard provided better results than that every 14 th day.Significance and impact of the study: Stilbenes from grapevine canes are good candidates as natural fungicides against downy mildew. Given the very large quantity of grape canes available each year, this strategy could be scaled up to control this grapevine disease in a sustainable manner.Abstract manuscript

show abstract

Oligosaccharides from Botrytis cinerea and Elicitation of Grapevine Defense

Cited by 11 publications

References 107 publications

Dual Mode of Action of Grape Cane Extracts against Botrytis cinerea

Dual Mode of Action of Grape Cane Extracts against Botrytis cinerea

A Plant Extract Acts Both as a Resistance Inducer and an Oomycide Against Grapevine Downy Mildew

<em>Vitis vinifera</em> canes, a source of stilbenoids against downy mildew

Contact Info

Product

Resources

About