Oligonucleotide Delivery by Cell‐Penetrating “Striped” Nanoparticles

Jewell, Christopher M.; Jung, Jin Mi; Atukorale, Prabhani U.; Carney, Randy P.; Stellacci, Francesco; Irvine, Darrell J.

doi:10.1002/anie.201104514

Cited by 74 publications

(75 citation statements)

References 25 publications

(17 reference statements)

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“…They found that the former particle with the ordered arrangement can directly permeate across the cell membrane without the membrane disruption, whereas the latter particle with the random arrangement was mostly trapped in endosomes by the endocytosis. A similar result has been observed in Jewell et al (2011). A possible explanation on this effect is that the ligand pattern in the ordered arrangement may be assumed to be more rigid, resulting in the direct permeation across the cell membrane (a more fluid layer) without the membrane disruption, which is similar mechanism for cell penetrating peptides (Verma et al, 2008).…”

Section: Key Physico-chemical Properties Of Nps For Their Direct Permsupporting

confidence: 71%

“…However, as shown in Table 1, the direct permeation of NPs has been observed regardless of the surface charge of the NPs: i.e., the NPs' surface charge may not be determinant for the direct permeation. As seen in Table 1, a positively charged NP exhibited higher uptake through the direct penetration pathway than neutral and negatively charged NPs (Arvizo et al, 2010;Cho et al, 2009), while a negatively charged NP exhibited the direct permeation (Jewell et al, 2011;Lund et al, 2011;Van Lehn et al, 2013;Verma et al, 2008). Van Lehn et al (2013) reported that the negatively charged gold NP directly penetrates into the synthetic vesicle without disruption of the vesicle membrane, while other studies reported that positively charged NPs can induce disruption of cell membranes, leading to cell death (Hong et al, 2004(Hong et al, , 2006.…”

Section: Key Physico-chemical Properties Of Nps For Their Direct Permmentioning

confidence: 99%

“…This hydrophobic interaction becomes higher at longer ligand length, resulting in reducing the minimum force for permeating the first layer (from hydrophilic to hydrophobic) and increasing the minimum force for permeating the second layer (from hydrophobic to hydrophilic). As presented in Section 2, some experimental studies exhibited that the NP direct permeation can be enhanced by surface modifications with orderly arranged ligand structural pattern (Jewell et al, 2011;Verma et al, 2008), while its mechanism is still unknown. This issue has been investigated by MD simulation studies.…”

Section: Biased MD Simulation Studiesmentioning

confidence: 99%

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Direct Permeation of Nanoparticles across Cell Membrane: A Review

Nakamura¹,

Watano²

2018

KONA

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Nanoparticles have attracted much attention as a key material for new biomedical and pharmaceutical applications. For success in these applications, the nanoparticles are required to translocate across the cell membrane and to reach to inside of the cell. Among several translocation pathways of nanoparticles, the direct permeation pathway has a great advantage due to its high delivery efficacy. However, despite many research efforts, key properties and factors for driving the direct permeation of nanoparticle and its underlying mechanisms are far from being understood. In this article, experimental and computational studies regarding the direct permeation of nanoparticles across a cell membrane will be reviewed. Firstly, experimental studies on the nanoparticle-cell interactions, where spontaneous direct permeation of nanoparticles was observed, are reviewed. From the experimental studies, potential key physico-chemical properties of nanoparticles for their direct permeation are discussed. Secondly, physical methods such as electroporation and sonoporation for delivering nanoparticles into cells are reviewed. Current status of technologies for facilitating the direct permeation of nanoparticle is presented. Finally, we review molecular dynamics simulation studies and present the latest findings on the underlying molecular mechanisms of the direct permeation of nanoparticle.

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Section: Key Physico-chemical Properties Of Nps For Their Direct Permsupporting

confidence: 71%

Section: Key Physico-chemical Properties Of Nps For Their Direct Permmentioning

confidence: 99%

Section: Biased MD Simulation Studiesmentioning

confidence: 99%

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Direct Permeation of Nanoparticles across Cell Membrane: A Review

Nakamura¹,

Watano²

2018

KONA

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“…These results were confirmed by intracellular photothermal heterodyne imaging of the same type of NPs [26]. Additionally recently we have shown that cell membrane penetrating particles can be used to cargo genetic materials in B16-F0 melanoma cells [27]. Finally, Lund et al [28] have recently shown that via an energy-independent diffusion across the cell membrane, gold NPs coated with a 1:1 molar mixture of PEG-NH 2 and glucose ligands are taken up 18 times faster than NPs coated with either PEG-NH2 or glucose.…”

Section: Introductionsupporting

confidence: 60%

“…Also only limited time points were studied, and the core of the experiments was based on either 4 h [27], 3 h [20], or 1 h [20,26] incubation times. To better establish the time evolution of NP uptake and to quantitatively compare between particle compositions and cell type, we have conducted here a set of systematic NP internalization experiments.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Cellular Uptake of Mixed-Monolayer Protected Nanoparticles

Carney

Mueller

et al. 2012

Biointerphases

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Nanoparticles (NPs) are gaining increasing attention for potential application in medicine; consequently, studying their interaction with cells is of central importance. We found that both ligand arrangement and composition on gold nanoparticles play a crucial role in their cellular internalization. In our previous investigation, we showed that 66-34OT nanoparticles coated with stripe-like domains of hydrophobic (octanethiol, OT, 34%) and hydrophilic (11-mercaptoundecane sulfonate, MUS, 66%) ligands permeated through the cellular lipid bilayer via passive diffusion, in addition to endo-/pino-cytosis. Here, we show an analysis of NP internalization by DC2.4, 3T3, and HeLa cells at two temperatures and multiple time points. We study four NPs that differ in their surface structures and ligand compositions and report on their cellular internalization by intracellular fluorescence quantification. Using confocal laser scanning microscopy we have found that all three cell types internalize the 66-34OT NPs more than particles coated only with MUS, or particles coated with a very similar coating but lacking any detectable ligand shell structure, or 'striped' particles but with a different composition (34-66OT) at multiple data points.

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Amphiphilic Polymeric Nanocarriers with Luminescent Gold Nanoclusters for Concurrent Bioimaging and Controlled Drug Release

Chen

Luo

et al. 2013

Adv Funct Materials

109

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Multifunctional theranostic systems with good biocompatibility, strong clinical imaging capability, and target specifi city are the desired features of future medicine. Here, the design of a theranostic nanocomposite capable of simultaneous targeting and imaging of the cancer cells is presented. It releases its drug payload by a controlled release mechanism. The nanocomposite contains luminescent gold nanocluster ( L -AuNC) photostable and biocompatible diagnostic probes conjugated to a folic acid (FA)-modifi ed pH-responsive amphiphilic polymeric system for controlled drug release. The nanocomposite uses a core-satellite structure to encapsulate hydrophobic drugs and releases the drug payload in mildly acidic endosomal/lysosomal compartments by the action of the pH-labile linkages in the polymer. In vivo studies show the selective accumulation of the FA-conjugated nanocomposite in tumor tissues by folate-receptor-mediated endocytosis. These fi ndings demonstrate the potential of the nanocomposite as a nontoxic, folate-targeting, pH-responsive drug carrier that is useful for the early detection and therapy of folate-overexpressing cancerous cells.

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Oligonucleotide Delivery by Cell‐Penetrating “Striped” Nanoparticles

Cited by 74 publications

References 25 publications

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Dynamic Cellular Uptake of Mixed-Monolayer Protected Nanoparticles

Amphiphilic Polymeric Nanocarriers with Luminescent Gold Nanoclusters for Concurrent Bioimaging and Controlled Drug Release

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