Oligonucleotide and Amplification Fingerprinting of Wild Species and Cultivars of Banana (Musa spp.)

Abstract

DNA oligonucleotide and amplification fingerprinting have been successfully used to detect genetic polymorphisms in 15 representative species and cultivars of the genus Musa, comprising AA, AAA, AAAA, AAB, ABB, and BB genotypes. In-gel-hybridization of Hinf I-digested genomic banana DNA to the 32P-labeled synthetic oligonucleotides (GATA)4, (GTG)5, and (CA)8 revealed considerable polymorphisms between Musa species and cultivars. The fingerprint patterns proved to be somatically stable and did not show differences between individual plants of 'Grand Nain' (AAA genotype). Dendrograms based on oligonucleotide fingerprint band sharing data proved to be consistent with most of the known features of the history of banana and plantain cultivation and evolution, respectively. DNA samples from the same banana species and cultivars were also amplified by PCR using single or pairwise combinations of short oligonucleotide primers. Amplification products were separated on agarose or polyacrylamide gels and visualized by ethidium bromide or silver staining, respectively. Polymorphic patterns were obtained with some but not all primers. By using the CCCTCTGCGG primer in simplex and/or duplex PCR, the induced mutant 'GN60A' was clearly recognized from its original variety 'Grand Nain'. Both fingerprint techniques allowed the detection of bands characteristic for the A and B genome. This DNA fingerprinting technology has potential application in several areas of Musa improvement.

Application of the RAPD technique in tilapia fish: species and subspecies identification

“…RAPD analysis has been used for constructing trees in other organisms (Chalmers et al, 1992;Kaemmer et al, 1992;Megnegneau et al, 1993;Tibayrenc et al, 1993). We have observed that small alterations in PCR parameters or quality of target DNA can radically alter RAPD patterns (see also, Devos & Gale, 1992; Williams et a!., 1993).…”

Genetic Markers and Plant Genetic Resource Management

“…Most studies (e.g., Kaemmer et al 1992) have reported on a more limited sample of clones than those held by major genebanks. During the next few years molecular technologies will be more widely employed for measuring the breadth and gaps in large collections of vegetatively propagated plants.…”

Distinction between Cold-sensitive and -tolerant Jute by DNA Polymorphisms

“…Random-amplified polymorphic DNA (RAPD) markers (Williams et al, 1990) have been successfully used for cultivar analysis in a number of plant species. These include the following: broccoli and cauliflower (Hu and Quiros 1991;Kresovich et al, 1992), cocoa (Wilde et al, 1992), banana (Kaemmer et al, 1992;Howell et al, 1994), papaya (Stiles et al, 1993), apple (Koller *To whom correspondence should be addressed. Tel: 88-2-966-1920-59 (6102);Fax: 88-2-861-5583 E-mail: haseena@bangla.net et al, 1993), celery (Yang and Quiros et al, 1993), onion (Wilkie et al, 1993), cranberry (Novy et al, 1994), sunflower (Lawson et al, 1994), rice (Takeuchi 1994), maize (Yazaki et al, 1994), Brassica juncea (Fujushiro et al, 1994), soybean (Lin, 1996;Powel, 1996), rose (Denbener et al, 1996), kenaf (Zhou et al, 1998;Cheng et al, 2000), roselle (Hanboonsong et al, 2000), and jute (Belayat et al, 2002).…”