Application of the RAPD technique in tilapia fish: species and subspecies identification

Bardakçı, Fevzi; Skibinski, D. O. F.

doi:10.1038/hdy.1994.110

Cited by 289 publications

(226 citation statements)

References 23 publications

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“…Table 3 shows the similarity values within-(on the diagonal) and between-populations (above the diagonal). Within-population indices were very high, ranging from 95.36% to 100%, and greater than those found among other species of fish using RAPD analysis (Bardakci and Skibinski, 1994;Foo et al, 1995;Bielawsky and Pumo, 1997). Likewise, the genetic similarity between populations of the same species was smaller than the intrapopulation similarities.…”

Section: Discussionmentioning

confidence: 93%

Phylogenetic relationships among Spanish Barbus species (Pisces, Cyprinidae) shown by RAPD markers

Callejas

Ochando

2002

Heredity

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Random amplified polymorphic DNA (RAPD) markers were used to estimate the population structure and phylogenetic relationships among the eight species of the genus Barbus that inhabit the Iberian Peninsula. Ten random oligodecamers were used to amplify DNA from 232 fish sampled from 15 populations. A total of 270 markers were detected that revealed low levels of genetic variability. The conclusions of cluster analysis indicate two main branches and three welldifferentiated groups: north-eastern, Mediterranean and

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Section: Discussionmentioning

confidence: 93%

Phylogenetic relationships among Spanish Barbus species (Pisces, Cyprinidae) shown by RAPD markers

Callejas

Ochando

2002

Heredity

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“…DNA was extracted from the fin clip using a slight modification of the method of Bardakci and Skibinski (1994).…”

Section: Methodsmentioning

confidence: 99%

Associations between single nucleotide polymorphisms in candidate genes and growth rate in Arctic charr (Salvelinus alpinus L.)

2003

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We tested for associations between single nucleotide polymorphisms (SNPs) in five candidate genes allied with the growth hormone axis and the age-specific growth rate of Arctic charr (Salvelinus alpinus L.: Salmonidae). Two large full sib families (N ¼ 217 and 95) were created by backcrossing males that were hybrids between two phenotypically divergent populations from Labrador, Canada and from Nauyuk Lake, Canada to females that were from Nauyuk Lake. Measures of individual growth rate (wet weight and fork length) were made three times during a 420-day period after the juveniles were transferred from 4 to 111C. We then identified SNP markers in 10 proposed candidate genes known to be related to the growth hormone axis. Comparative alignments of amino-acid sequences and nucleotide sequences from other fish species were used to design PCR primers that would amplify 0.5-3 kb DNA regions of the candidate genes. All the individuals in the two backcross families were genotyped for these SNP markers using either polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) or bidirectional amplification of specific alleles (Bi-PASA) approaches. A significant association between a particular SNP allele and early growth was found for the locus containing the growth hormone-releasing hormone and pituitary adenylate cyclase-activating polypeptide genes (GHRH/PACAP2, P ¼ 0.00001). We argue that using comparative sequence information to design PCR primers for candidate genes is an efficient method for locating quantitative triat loci in nonmodel organisms.

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“…Genomic DNA was extracted by an NaCl protocol (Bardakci and Skibinski, 1994) and then placed in 30 µL TE buffer (0.5 M Tris-EDTA). The samples were quantified using a fluorimeter (Qubit -Life Technologies, Grand Island, NY, USA) and diluted to 5 ng/µL.…”

Section: Dna Extraction and Pcr-rapdmentioning

confidence: 99%

Genetic analysis of five sedentary fish species in middle Laranjinha River (upper Paraná River basin): A case study

Frantine‐Silva¹,

Ferreira²,

Nascimento³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Most studies of diversity and genetic structure in neotropical fish have focused on commercial species from large rivers or their reservoirs. However, smaller tributaries have been identified as an important alternative migratory route, with independent pools of genetic diversity. In this context, the present study aimed to evaluate genetic diversity and structure in five neotropical fish species from a region of Laranjinha River in the upper Paraná River basin. PCR-RAPD (random amplified polymorphic DNA) markers were used to characterize around 40 individuals of each species distributed upstream and downstream of Corredeira Dam that interrupts the river. The descriptive index of genetic diversity (P = 30.5-82%; H E 0.122-0.312) showed that the populations have acceptable levels of genetic diversity. The values for Nei's genetic distance (D N min 0.0110 and max 0.0306) as well as the genetic structure index and the analysis of molecular variance (AMOVA, ϕ ST min 0.0132 and max 0.0385) demonstrated low, but significant levels of genetic structure. Bayesian analysis of assignment found two k clusters, including several individuals with mixed ancestry for all populations from the five species analyzed. These findings along with historical data on rainfall and the low dimensions of the dam studied here support the hypothesis that periodic floods enable the transit of individuals between different localities mitigating the differentiation process between populations.

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Application of the RAPD technique in tilapia fish: species and subspecies identification

Cited by 289 publications

References 23 publications

Phylogenetic relationships among Spanish Barbus species (Pisces, Cyprinidae) shown by RAPD markers

Phylogenetic relationships among Spanish Barbus species (Pisces, Cyprinidae) shown by RAPD markers

Associations between single nucleotide polymorphisms in candidate genes and growth rate in Arctic charr (Salvelinus alpinus L.)

Genetic analysis of five sedentary fish species in middle Laranjinha River (upper Paraná River basin): A case study

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