Oligomerisation of the Developmental Regulator Proline Rich Homeodomain (PRH/Hex) is Mediated by a Novel Proline-rich Dimerisation Domain

PRH/HHex inhibits the migration of breast and prostate epithelial cells through direct transcriptional regulation of Endoglin

“…We infected the cells with an empty adenovirus or with an adenovirus that expresses Myc-tagged PRH (Ad PRH) (28) . In both PNT2-C2 cells and PC3 cells infection with Ad PRH brings about a significant increase in Endoglin mRNA levels (Fig.3A).…”

“…The Endoglin expression vector pcDNA3.1-Endoglin (long isoform) was a gift from Professor Clare Isacke. The recombinant adenoviral construct expressing Myc-PRH has been described previously (28). Heat-Inactivated (Sigma), 20ng/ml EGF (Peprotech) 0.5ug/ml Hydrocortisone (Sigma), 100ng/ml Cholera toxin (Sigma), 10g/ml Insulin (Sigma) and 1% Penicillin/Streptomycin.…”

PRH/Hhex Controls Cell Survival through Coordinate Transcriptional Regulation of Vascular Endothelial Growth Factor Signaling

“…SELEX (systematic evolution of ligands by exponential enrichment) experiments with random oligonucleotides and a truncated protein consisting of the PRH homeodomain and C-terminal region have identified a consensus DNA binding sequence 5ЈC/t A/tATTAAA/g3Ј (lowercase letters indicate base pairs found less frequently) (9). Unlike the truncated PRH protein used in the SELEX experiments, full-length PRH forms oligomers and binds with high affinity to promoter DNA containing multiple 5ЈATTA3Ј sequences that do not match the consensus sequence (64,76). To assess whether PRH is likely to bind to sites within the regulatory regions of the Vegfr-1, Vegfr-2, and Vegf genes, we analyzed the sequence 5,000 bp upstream of the first exon for the presence of core 5ЈATTA3Ј sequences.…”

“…PRH is an oligomeric protein and has a high affinity for multiple clustered PRH binding sites (64,76). PRH acts as a context-dependent transcription factor to activate or repress transcription depending on its target gene (62).…”

Gene Expression and Regulation in Mammalian Cells - Transcription From General Aspects

“…The PRH protein forms oligomeric complexes in vitro and in cells [20]. The PRH N-terminal domain is resistant to SDS (sodium dodecyl sulphate)-induced denaturation and does not have extensive α-helical or β-sheet secondary structures.…”