Oligoglycine and fluoropolymer functionalized enzyme-responsive gene delivery surface for rapid in situ endothelialization of vascular grafts

Amphiphilic multi-targeting copolymer micelles efficiently deliver pZNF580 to promote endothelial cell proliferation and migration

“…29 Hao et al designed three tandem peptides REDV-Gm-TAT-Gm-NLS (m = 0, 1, and 4) to form a complex with the pZNF580 plasmid by integrating the REDV, TAT, and NLS peptides and introducing glycine with different numbers of repetitions as a flexible fragment, which showed that the introduction of the targeted REDV and glycine sequences led to an improvement in the endothelialization efficiency. 22,30 These results demonstrated that the construction of gene delivery systems with bioactive peptides such as REDV, TAT, and NLS could effectively improve the efficiency of gene delivery. However, the novel gene vectors still have disadvantages such as difficult endosome escape and low transfection efficiency.…”

Chitosan Hydrogel as Tissue Engineering Scaffolds for Vascular Regeneration Applications

“…Drugs, gaseous signal molecules, genes, and microRNAs are commonly employed to modulate cell behaviors for the regeneration and tissue engineering, such as cell adhesion, growth, proliferation, and migration. Pharmaceutical molecule and gene therapies offer a promising strategy for promoting angiogenesis and accelerating endothelialization in tissue engineering [225][226][227][228][229][230][231][232][233][234][235][236][237][238][239][240][241][242][243].…”

Restoring endothelial function: shedding light on cardiovascular stent development

“…Our group has developed the enzyme-responsive gene-eluting stents. [155][156][157] By virtue of a matrix metalloproteinase cleavable peptide (MCP) as a linker, gene complex nanoparticles could be released in response to matrix metalloproteinase (MMP) secreted by ECs, thus accomplishing the goal of continuously regulating the behavior of ECs and strengthening endothelialization (Fig. 11A).…”