Oligoclonal bands in multiple sclerosis cerebrospinal fluid: An update on methodology and clinical usefulness

Link, Hans; Huang, Yu‐Min

doi:10.1016/j.jneuroim.2006.07.006

Cited by 328 publications

(269 citation statements)

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“…Our results may support this concept since we found significant correlation between CSF and serum UA concentrations in MS patients, but not in controls. Additionally, we have shown significant correlation between the CSF/serum albumin quotient, a marker for disruption of the BBB (35), and CSF UA concentrations in MS patients, but not in controls. Disruption of the BBB is one of the key events in the immunoinflammatory processes in MS (36).…”

Section: Discussionmentioning

confidence: 70%

Cerebrospinal fluid and serum uric acid levels in patients with multiple sclerosis

Dujmović¹,

Gazibara²,

Obrenović³

et al. 2009

Clinical Chemistry and Laboratory Medicine

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Background: Peroxynitrite was hypothesized to be involved in the pathogenesis of multiple sclerosis (MS) through its various neurotoxic effects. Uric acid (UA) was shown to be a strong peroxynitrite scavenger. Methods: We analyzed cerebrospinal fluid (CSF) and serum UA concentrations in 30 MS patients and 20 controls with non-inflammatory neurological diseases (NIND) and correlated these findings with demographic and clinical characteristics of MS patients. Disease activity was assessed by brain magnetic resonance imaging (MRI) and the CSF/serum albumin quotient as an indicator of the state of blood-brainbarrier (BBB). Results: Serum UA concentrations were found to be significantly lower in MS patients compared with controls (ps0.019). CSF UA concentrations were lower in MS patients as compared to controls, as well as in patients with active MS (clinical and/or MRI activity) in comparison to patients with inactive MS or controls, but these differences were not statistically significant. Significant correlation was found between CSF and serum UA concentrations (ps0.016) in MS patients, but not in controls; and between CSF UA concentrations and the CSF/serum albumin quotient in MS patients (ps0.043), but not in controls. Conclusions: Our results support the significance of UA in the pathogenesis of MS. Decreased serum UA concentrations in MS patients might be due to both intrinsically reduced antioxidant capacity and increased UA consumption in MS. CSF UA concentrations may not be a reliable marker of disease activity in MS since its concentration is dependent on leakage

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Section: Discussionmentioning

confidence: 70%

Cerebrospinal fluid and serum uric acid levels in patients with multiple sclerosis

Dujmović¹,

Gazibara²,

Obrenović³

et al. 2009

Clinical Chemistry and Laboratory Medicine

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“…This information may be helpful for distinction when images seen at MRI may correspond to acute lesions or only to cicatricial demyelination; (4) the graphic representation of samples from patients with IgG immunoproduction is displayed in a similar way to that of the Link's IgG index analysis (region 4 of Fig 2). Although CSF analysis in MS aims to characterize the local immune response in the CNS, a number of other features may differentiate MS from other diseases of the CNS, such as: (1) in MS, there is usually a high number of OCB; (2) in MS, the simultaneous presence of OCB in CSF and in the blood serum is uncommon, even if a high number of bands have been identified in the CSF; (3) there usually is a normal number of cell count or a slight pleocytosis, usually below 30 cells/mm 3 , with an absolute predominance of lymphomononuclear cells; (4) the total protein content is usually normal or slightly above normal, generally below 70 mg/dL; (5) the concentration of glucose should be within reference limits [2][3][4]20 .…”

Section: Resultsmentioning

confidence: 99%

“…The CSF analysis included the classical routine tests (cytomorphological profile; determination of total protein content; dosage of glucose) and immunological reactions for the infectious diseases more frequently observed in tropical countries 2,3,6 .…”

Section: Methodsmentioning

confidence: 99%

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Study of oligoclonal bands restricted to the cerebrospinal fluid in multiple sclerosis patients in the city of São Paulo

Gama

Machado

Livramento

et al. 2009

Arq. Neuro-Psiquiatr.

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-The frequency of oligoclonal bands (OCB) restricted to the cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) varies widely in different populations. The objective of this study was to determine the frequency of these OCB in a group of MS patients in the city of São Paulo. Techniques used to detect OCB consisted of isoelectric focusing followed by immunoblotting. Oligoclonal bands were found in 49 (54.4%) out of 90 patients with clinically definite MS; in (31.2%) of the 16 patients with clinically isolated syndrome; in 7 (17.9%) of 39 patients with inflammatory disorders of the central nervous system (IDCNS), and in none of the individuals with no neurological condition (control group). The specificity of the method was 100% when compared to the control group and 82.1% when compared to the IDCNS group. These results suggest that the frequency of CSF OCB is much lower in Brazilian MS patients from São Paulo city than that reported in MS series from Caucasian populations.KEY WORDS: multiple sclerosis, cerebrospinal fluid, oligoclonal bands, Brazilian population. estudo de bandas oligoclonais restritas ao líquido cefalorraquidiano em pacientes com esclerose múltipla na cidade de São paulo resumo -A frequência da detecção de bandas oligoclonais (BOC) restritas ao líquido cerebrorraquidiano (LCR) em pacientes com esclerose múltipla (EM) varia amplamente em diferentes populações. O objetivo deste estudo foi determinar a frequência destas BOC em pacientes com EM em amostra de população da cidade de São Paulo. A técnica utilizada para a detecção das BOC foi a focalização isoelétrica, seguida do immunoblotting. BOC foram detectadas: em 49 (54,4%) de 90 pacientes com EM clinicamente definida; em 5 (31,2%) de 16 pacientes com síndrome clínica isolada; em 7 (17,9%) de 30 pacientes com doenças inflamatórias do sistema nervoso central (DISNC); e em nenhum indivíduo sem doença neurológica. A especificidade do método foi 100% quando comparada ao grupo controle e 82,1% quando comparada ao grupo de DISNC. Estes resultados sugerem que a freqüência de BOC no LCR é mais baixa em pacientes da cidade de São Paulo portadores de EM do que aquelas descritas em populações caucasianas. PALAVRAS-CHAVE: esclerose múltipla, líquido cefalorraquidiano, bandas oligoclonais, população brasileira.

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“…Multiple sclerosis (MS) is a chronic inflammatory immune-mediated and demyelinating disease of the central nervous system (CNS) [1][2][3] . The frequency of MS in Brazil varies among different regions due to ethnic diversity and the diversity of environmental and socioeconomic characteristics due to the country's large territorial extent [4][5][6] .…”

mentioning

confidence: 99%

Antiparasitic antibodies occur with similar frequency in patients with clinically established multiple sclerosis with or without oligoclonal bands in the cerebrospinal fluid

Fonseca-Papavero¹,

Callegaro²,

Gama

et al. 2013

Arq. Neuro-Psiquiatr.

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Anticorpos antiparasitários ocorrem com frequência semelhante em pacientes com esclerose múltipla quando há e quando não há bandas oligoclonais no líquido cefalorraquidiano ABSTRACTThe "hygiene hypothesis" postulates an inverse relationship between the prevalence of parasitic infections and the frequency of multiple sclerosis (MS). Objective: It was to study whether antibodies against parasites could be demonstrated more frequently in blood serum from MS patients with oligoclonal bands (OCB) than from MS patients without OCB. Methods: We studied serum samples from 164 patients who had previously been analyzed to investigate OCB. Parasitic antibodies were studied through unidimensional electrophoresis of proteins on polyacrylamide gel against Taenia antigens, searching for antiparasitic specific low molecular weight antibodies and also for antiparasitic nonspecific high molecular weight antibodies. Results: Two of the 103 patients with no evidence of OCB had antibodies of low molecular weight and 59 of them had antibodies of high molecular weight. Of the 61 patients with evidence of OCB, one showed antibodies of low molecular weight and 16 showed antibodies of high molecular weight. Conclusion: Antiparasitic antibodies are detected with similar frequency in MS patients with OCB and in MS patients without OCB.Key words: hygiene hypothesis, multiple sclerosis, oligoclonal bands, Taenia antigens, antiparasitic antibodies. RESUMOA "hipótese da higiene" postula haver relação inversamente proporcional entre a prevalência de infecções por parasitas e a frequência da esclerose múltipla (EM). Objetivo: Foi verificar se em pacientes com EM aparecem anticorpos antiparasitários mais frequentemente no grupo com bandas oligoclonais (BOC) do que no grupo sem BOC. Métodos: Foram estudadas amostras de sangue de 164 pacientes previamente analisadas para investigar BOC. Foi feita eletroforese unidimensional de proteínas em gel de poliacrilamida contra antígenos de Taenia para pesquisa de anticorpos específicos de baixo peso molecular e também de anticorpos inespecíficos de alto peso molecular. Resultados: Dois dos 103 pacientes em que não havia BOC apresentaram anticorpos de baixo peso molecular, e 59 apresentaram anticorpos de alto peso molecular. Dos 61 pacientes em que não havia BOC, um apresentou anticorpos de baixo peso molecular e 16, anticorpos de alto peso molecular. Conclusão: Anticorpos antiparasitários foram detectados com frequência semelhante em doentes com EM independentemente da presença ou não de BOC.Palavras-Chave: hipótese da higiene, esclerose múltipla, bandas oligoclonais, antígenos de Taenia, anticorpos antiparasitários. Multiple sclerosis (MS) is a chronic inflammatory immune-mediated and demyelinating disease of the central nervous system (CNS) [1][2][3] . The frequency of MS in Brazil varies among different regions due to ethnic diversity and the diversity of environmental and socioeconomic characteristics due to the country's large territorial extent [4][5][6] . A recent study on patients with MS in the ci...

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Oligoclonal bands in multiple sclerosis cerebrospinal fluid: An update on methodology and clinical usefulness

Cited by 328 publications

References 50 publications

Cerebrospinal fluid and serum uric acid levels in patients with multiple sclerosis

Cerebrospinal fluid and serum uric acid levels in patients with multiple sclerosis

Study of oligoclonal bands restricted to the cerebrospinal fluid in multiple sclerosis patients in the city of São Paulo

Antiparasitic antibodies occur with similar frequency in patients with clinically established multiple sclerosis with or without oligoclonal bands in the cerebrospinal fluid

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