Oligo-2',5'-adenylate Synthetase Activity in K562 Cell Lines Persistently Infected with Measles or Mumps Virus

Fujii, Nobuhiro; Oguma, Keiji; Kimura, Kouichi; Yamashita, Toshiharu; Ishida, Setsuko; Fujinaga, Kei; Yashiki, Teruo

doi:10.1099/0022-1317-69-8-2085

Cited by 32 publications

(15 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, our results showed that IL-6 and IFN peaked early, on day 1 of admission ( Figure 2). Previous publications have shown the critical roles of IL-6 and IFN in viral infections such as in mumps [19][20][21][22], results that are consistent with our findings in varicella infections. These cytokines may be used as serum markers to make early diagnosis of skin infections or liver damage in varicella and to take early measures during the course of the disease.…”

Section: Discussionsupporting

confidence: 93%

Cytokine levels are associated with the severity of varicella infections

Hao

Wang

et al. 2015

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Varicella is a highly contagious disease. Epidemics of varicella are seen every year globally and present a threat to public health, especially in China and other developing countries. Methodology: Clinical and laboratory findings of 865 varicella patients admitted to Beijing You'an Hospital, China, between January 2011 and December 2013 were collected and analyzed. Patients with isolated complication were grouped as SI (skin infection, n = 132) and LD (liver damage, n = 89). Two hundred and one patients without complications were grouped as control (mild group). Levels of T-cell subtypes and eight serum cytokines and were also tested. Levels of IFN and IL-6 were monitored prospectively in another 12 grouped patients. Results: SI was complicated in 21.7% (188/865) of varicella cases, and LD was complicated in 16.8% (145/865). The rates of SI and LD in varicella patients increased rapidly in the past three years. No laboratory findings were associated with SI or LD (all p > 0.05). IL-6 and IFN levels were correlated with amniotic membrane extract (AME) (p = 0.044 and p = 0.038). Their levels peaked at day 1 of admission, and then started to decline. Conclusions: The incidence of serious complications has become more common in recent years. IL-6 and IFN may possibly be used as early serum markers for identifying patients at risk of developing complications such as skin infections in varicella.

show abstract

Section: Discussionsupporting

confidence: 93%

Cytokine levels are associated with the severity of varicella infections

Hao

Wang

et al. 2015

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…Cells persistently infected with mumps virus were established as described previously (41,42). Briefly, OSC19 and OSC70 cells, infected with mumps virus vaccine strain Torii at a multiplicity of infection of 0.1, were cultured and passaged every 5 or 7 days.…”

Section: Methodsmentioning

confidence: 99%

Suppression of Thermotolerance in Mumps Virus-infected Cells Is Caused by Lack of HSP27 Induction Contributed by STAT-1

Yokota

Yokosawa

Kubota

et al. 2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

Viral infection modulates the regulation of apoptosis in host cells. Here, we report a novel mechanism by which human cells infected with mumps virus become susceptible to apoptosis caused by extracellular stresses. Mumps virus stimulates proteasome-dependent degradation of STAT-1 by action of viral accessory protein V, resulting in a severe decrease in STAT-1 protein in infected cells. We exposed mumps virus-infected and uninfected cells to heat and chemical stress. The infected cells failed to acquire resistance to apoptotic stimuli (thermotolerance) after exposure to these mild stresses. The induction of HSP27 by stress exposure was dramatically suppressed in the infected cells, but HSP70 induction was not affected. STAT-1 was required for transcriptional activation of the HSP27 gene, but not for the HSP70 gene, and cDNA transfection of STAT-1 in mumps virus-infected cells restored thermotolerance. Phosphorylated heat shock factor-1 (HSF-1) and STAT-1 phosphorylated on neither tyrosine nor serine residues were co-transported to the nucleus in response to stress. Furthermore, overexpression of unphosphorylatable mutants of STAT-1 also restored thermotolerance in mumps virus-infected cells. These lines of evidence indicate that the induction of HSP27 by stress requires STAT-1 in addition to the activated HSF-1. Furthermore, STAT-1 required for the induction of HSP27 worked independent to its phosphorylation. Thus, HSP27-dependent thermotolerance is suppressed by mumps virus infection through the destruction of STAT-1. The lack of thermotolerance should allow the infected cells to be eliminated by apoptosis and might be a host defense against viral infection.

show abstract

“…The bronchiolar carcinoma cell line A549 and the cervical squamous carcinoma cell line SiHa were obtained from the American Type Culture Collection (Manassas, Va.). MeV strains Edmonston, Hälle, Schwarz, and CAM70 were described previously (4,6). Strains AK1 (24) and SMU were clinical isolates obtained from throat swabs of measles patients, using B95a cells and Vero cells for propagation, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Strains AK1 (24) and SMU were clinical isolates obtained from throat swabs of measles patients, using B95a cells and Vero cells for propagation, respectively. SiHa cell lines persistently infected with MeV were established as previously described (4,6). Virus propagation and titer determination of MeV strains, except AK1, were performed by using Vero cells as indicator cells.…”

Section: Methodsmentioning

confidence: 99%