Olfactory learning and memory in <i><scp>G</scp>rapholita molesta</i>

Sant’Ana, Josué; Gregório, Patrícia Luciane Fernandes

doi:10.1111/eea.12456

Cited by 2 publications

(1 citation statement)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The females were found to retain information from apple substrate for at least a 12‐h period. Memory retention in insects can vary from a minute to weeks (Gandolfi et al, 2003; Sant'Ana & Gregório, 2016; de Bruijn et al, 2018). Usually, the value of the host's reward determines the memory retention time (Bleeker et al, 2006; Kruidhof et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Chemotaxis and parasitism of the Neotropical fruit fly parasitoidGanaspis pelleranoican be altered by pre‐imaginal and imaginal conditioning to fruit volatiles

Pires

Sant’Ana

Leite

2022

Entomologia Exp Applicata

View full text Add to dashboard Cite

Search behavior of parasitoids can be triggered by fruit volatiles' perception and modified through pre-imaginal and/or imaginal conditioning. Ganaspis pelleranoi (Brèthes) (Hymenoptera: Figitidae) is an endoparasitoid of tephritid fruit flies of economic importance. Despite its relevance to agroecosystems, no studies about its learning processes have been done. Therefore, we conducted chemotaxis and parasitism bioassays to evaluate pre-imaginal and imaginal learning, and memory retention of G. pelleranoi females to volatiles of guava (Psidium guajava L., Myrtaceae) and apple (Malus domestica Borkh., Rosaceae). Parasitoids conditioned as immatures were obtained from Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) larvae reared in apple or artificial diet. Parasitoids conditioned as adults were exposed to an apple substrate for 24 or 48 h, and tested after 1, 12, and 24 h. In the olfactometer, parasitoids were given a choice between guava and apple fruits (infested and uninfested). To assess parasitism, three units of parasitism were offered, which consisted of A. fraterculus larvae brushed with water, or with apple or guava pulp. Females preferred guava volatiles regardless of their pre-imaginal conditioning. When adults were exposed to an apple substrate for 48 h, they preferred volatiles from this fruit for up to 12 h. Females that were conditioned for 24 or 48 h on apple volatiles parasitized more larvae in units with this substrate. Also, the number of flies that emerged from units with apple was lower for females conditioned for 48 h on the same fruit. Therefore, we conclude that pre-imaginal conditioning in infested fruits was less important than the parasitoid experience to chemicals of fruit volatiles during the adult stage. Also, the longer the exposure of adults to odors, the greater the attraction to those odors and memory retention. Conditioning of G. pelleranoi females could be used as a strategy to improve future biocontrol programs of fruit flies.

show abstract

Section: Discussionmentioning

confidence: 99%

Chemotaxis and parasitism of the Neotropical fruit fly parasitoidGanaspis pelleranoican be altered by pre‐imaginal and imaginal conditioning to fruit volatiles

Pires

Sant’Ana

Leite

2022

Entomologia Exp Applicata

View full text Add to dashboard Cite

show abstract

Associative learning and memory through metamorphosis inGrapholita molesta(Busck) (Lepidoptera: Tortricidae)

Sant’Ana¹,

Tognon²,

Pires³

et al. 2021

Can Entomol

Self Cite

View full text Add to dashboard Cite

Learning of chemical stimuli by insects can occur during the larval or adult life stage, resulting in changes in the imago chemotaxic behaviour. There is little information on learning in Tortricidae, and associative learning through metamorphosis is unknown in this group. Here, we evaluate the influence of olfactory aversive learning in Grapholita molesta (Busck) (Lepidoptera: Tortricidae) during the immature stage and determine if memory persists after metamorphosis. Larvae (10–12 days old) were conditioned to associate the odour of ethyl acetate with pulses of aversive electric shock. Insects were exposed to air, to the ethyl acetate odour, and to shock, in isolation or combination. After conditioning, both larvae and adults were tested in a two-choice olfactometer. Larvae exposed only to air or ethyl acetate increased legibility. Larvae trained with ethyl acetate and shock simultaneously exhibited significant avoidance to ethyl acetate. Avoidance was still present for at least 72 hours after metamorphosis. Thus, G. molesta has the ability to associate an odour to an aversive stimulus precociously, and this association is maintained through metamorphosis and persists into adulthood.

show abstract

Olfactory learning and memory in Grapholita molesta

Cited by 2 publications

References 43 publications

Chemotaxis and parasitism of the Neotropical fruit fly parasitoidGanaspis pelleranoican be altered by pre‐imaginal and imaginal conditioning to fruit volatiles

Chemotaxis and parasitism of the Neotropical fruit fly parasitoidGanaspis pelleranoican be altered by pre‐imaginal and imaginal conditioning to fruit volatiles

Associative learning and memory through metamorphosis inGrapholita molesta(Busck) (Lepidoptera: Tortricidae)

Contact Info

Product

Resources

About