Olfactory biosensor using odorant-binding proteins from honeybee: Ligands of floral odors and pheromones detection by electrochemical impedance

Lu, Yanli; Li, Hongliang; Zhuang, Shulin; Zhang, Diming; Zhang, Qian; Zhou, Jie; Dong, Siyuan; Liu, Qingjun; Wang, Ping

doi:10.1016/j.snb.2013.11.045

Cited by 67 publications

(49 citation statements)

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“…With the molecular docking models, the most related amino acid sequences, and the correlations between the conformational changes of protein and biosensors can be discussed. The detailed processes have been discussed in our previous papers [14,15].…”

Section: Modeling Of the Odorant Binding Proteins To Ligandsmentioning

confidence: 99%

“…Although cell-free expression system show potential to solve this problem, it was still difficult to produce functional receptors and stabilize them for a sufficient period of time [33]. For OBPs, however, the recombinant OBPs, such as rat OBP, porcine OBP, and insect OBPs, have been expressed in bacteria as well as in yeast in high yields, making their production simple and economical [14,15,[34][35][36].…”

Section: Production and Fixation Of Odorant Binding Proteinsmentioning

confidence: 99%

“…For mammal OBPs, such as bovine OBP and porcine OBP, they have already been studied in biosensors for odor detections, such as ethanol, octenol, and carvone, with high specificity and sensitivity [5,7,[17][18][19]. For insects, studies show that OBPs of honeybee and fruit fly could be used to detect floral odors, pheromones, and semiochemicals, in vitro [11,14,15,20]. Excitingly, recent studies show that peptide sequences derived from OBPs of Drosophila and honeybee (Apismellifera), and some mutant OBPs have also been used to detect chemicals, such as alcohols and trinitrotoluene (TNT) [11,20].…”

Section: Introductionmentioning

confidence: 99%

“…It offers great help in designing specific biosensors for odorant detections. With odorants, even that did not occur in the insects' natural environment, such as volatile compounds emitted from cancer cells [21,22], have been successfully detected, OBPs-based biosensors provide promising approaches for chemical molecular sensing, as well as for precise binding functions investigating this kind of special olfactory protein [15].…”

Section: Introductionmentioning

confidence: 99%

“…Based on our previous studies of OBPs [14,15], this chapter will give a detailed illustration about the development of OBPs-based biosensors, including how to isolate and purify OBPs, analysis of electrical characteristics of OBPs, the development of OBPs-based biosensors, and their promising applications.…”

Section: Introductionmentioning

confidence: 99%

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Smell Sensors Based on Odorant Binding Proteins

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Section: Modeling Of the Odorant Binding Proteins To Ligandsmentioning

confidence: 99%

Section: Production and Fixation Of Odorant Binding Proteinsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Smell Sensors Based on Odorant Binding Proteins

Yao

Liu

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Recognition and Quantification of Some Monoamines Neurotransmitters

Elmosallamy

Saber

2016

Electroanalysis

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1IntroductionNeurotransmitters are biochemical substancest hat are produced and secreted by an euron and then diffused across as ynapse to cause excitation or inhibition of other neurons. Dopamine,s erotonin, and epinephrine are exampleso fn eurotransmitters.D opamine (DA), 4-(2-aminoethyl) benzene-1,2-diol ( Fig.1)i sa no rganicc ompound of the catechol amine that plays important roles in the human brain and body.T he brain comprises several distinct dopamine systems, one of which plays an important role in reward-motivated behavior.M ost types of reward increase the level of dopamine in the brain.O ther brain dopamine systems control the release of various hormones and involvem otor control. Several diseases of the nervouss ystema re due to the dysfunctionso ft he dopamine system. Lacko fd opaminel evels in the body can cause Parkinson,s disease,h ighly altered levels of dopamine activity cause schizophrenia [1].O nt he otherh and, there are severalm ethods reported in the literature for the quantification of DA comprising voltammetry [2-6], UHPLC[ 7-10],H PLC [11-13],G C[ 14],c apillary electrophoresis [15-17],f luorimetry[ 18-22],e lectrochemiluminescence [23, 24],a ll of these methods are of highly costly,t ime consuming and involve derivatization steps. Biosensors [25] and potentiometric sensors [26-29] have also been reported.Serotonin, 3-(2-aminoethyl) indol-5-ol, or 5-hydroxytryptamine (5-HT) ( Fig.1)i samonoamine neurotransmitter.I ti sp rimarily found in the gastrointestinal tract, blood platelets and the centraln ervous system of animal including humans.5 -HT is popularly thoughtt obe ac on-tributor to feelingso fw ell-being and happiness [ 30].T he most widely used methods for the quantification of serotonin are UHPLC [ 7,8],H PLC [31-33],G C[ 14],c apillary electrophoresis [15],v oltammetry [6,[34][35]and fluorimetry [36].S of ar,t here is no potentiometric sensorf or quantificationo fs erotonin in the literature.Epinephrine known also as adrenaline,( R)-4-(1-hydroxy-2-(methylamino) ethyl) benzene-1,2-diol( Fig. 1) is ah ormone and an eurotransmitter [37],e pinephrine and norepinephrine are separate but related hormones secreted by the medulla of the adrenal glands.T he word adrenalinei su sed in commonp arlancet od enotei ncreaseda ctivation of the sympathetics ystem associated with the energy and excitement of the fight-or-fight response [38]. Ther eported methodsf or the determination of adrenaline are voltammetry [ 5,39],U PLC [7,8,10],H PLC [13,12],c apillary electrophoresis [15,17] and fluorimetry [22].A lso therei sn op otentiometric sensor for the deter-Abstract:T hree neurotransmitter sensors for dopamine, serotonin, and epinephrine have been developed. Potentiometric sensors for serotonin and epinephrinea re novel and first to be introduced. Then eurotransmitter potentiometric sensors are based on using dibenzo-30-crown-10 (DB30C10), dibenzo-24-crown-8 (DB24C8), and dibenzo-18-crown-6 (DB18C6) as ionophores and neutral carriers incorporating in poly(vinyl chloride) matrix membrane plastici...

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Nanoplasmonic Biosensor Using Localized Surface Plasmon Resonance Spectroscopy for Biochemical Detection

Zhang

et al. 2017

Biosensors and Biodetection

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Localized surface plasmon resonance (LSPR) associated with metal nanostructures has developed into a highly useful sensor technique. Optical LSPR spectroscopy of nanostructures often shows sharp absorption and scattering peaks, which can be used to probe several bio-molecular interactions. Here, we report nanoplasmonic biosensors using LSPR on nanocup arrays (nanoCA) to recognize bio-molecular binding for biochemical detection. These sensors can be modified to quantify binding of small molecules to proteins for odorant and explosive detections. Electrochemical LSPR biosensors can also be designed by coupling electrochemistry and LSPR spectroscopy measurements. Multiple sensing information can be obtained and electrochemical LSPR property can be investigated for biosensors. In some applications, the electrochemical LSPR biosensor can be used to quantify immunoreactions and enzymatic activity. The biosensors exhibit better performance than those of conventional optical LSPR measurements. With multi-transducers, the nanoplasmonic biosensor can provide a promising approach for bio-detection in environmental monitoring, healthcare diagnostics, and food quality control.

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Olfactory biosensor using odorant-binding proteins from honeybee: Ligands of floral odors and pheromones detection by electrochemical impedance

Cited by 67 publications

References 40 publications

Smell Sensors Based on Odorant Binding Proteins

Smell Sensors Based on Odorant Binding Proteins

Recognition and Quantification of Some Monoamines Neurotransmitters

Nanoplasmonic Biosensor Using Localized Surface Plasmon Resonance Spectroscopy for Biochemical Detection

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