Olfactory biosensor for insect semiochemicals analysis by impedance sensing of odorant-binding proteins on interdigitated electrodes

Lu, Yanli; Yao, Yao; Zhang, Qian; Zhang, Diming; Zhuang, Shulin; Li, Hongliang; Liu, Qingjun

doi:10.1016/j.bios.2014.09.098

Cited by 65 publications

(42 citation statements)

References 54 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the molecular docking models, the most related amino acid sequences, and the correlations between the conformational changes of protein and biosensors can be discussed. The detailed processes have been discussed in our previous papers [14,15].…”

Section: Modeling Of the Odorant Binding Proteins To Ligandsmentioning

confidence: 99%

“…For mammal OBPs, such as bovine OBP and porcine OBP, they have already been studied in biosensors for odor detections, such as ethanol, octenol, and carvone, with high specificity and sensitivity [5,7,[17][18][19]. For insects, studies show that OBPs of honeybee and fruit fly could be used to detect floral odors, pheromones, and semiochemicals, in vitro [11,14,15,20]. Excitingly, recent studies show that peptide sequences derived from OBPs of Drosophila and honeybee (Apismellifera), and some mutant OBPs have also been used to detect chemicals, such as alcohols and trinitrotoluene (TNT) [11,20].…”

Section: Introductionmentioning

confidence: 98%

“…Based on our previous studies of OBPs [14,15], this chapter will give a detailed illustration about the development of OBPs-based biosensors, including how to isolate and purify OBPs, analysis of electrical characteristics of OBPs, the development of OBPs-based biosensors, and their promising applications.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Smell Sensors Based on Odorant Binding Proteins

Yao

Liu

2015

Bioinspired Smell and Taste Sensors

Self Cite

View full text Add to dashboard Cite

Section: Modeling Of the Odorant Binding Proteins To Ligandsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Smell Sensors Based on Odorant Binding Proteins

Yao

Liu

2015

Bioinspired Smell and Taste Sensors

Self Cite

View full text Add to dashboard Cite

“…In addition to the natural surface-based sensors, insects have a delicate sense of smell that allows them to sensitively and selectively detect thousands of chemical pheromones at very low concentrations through their remarkable olfactory system, which has been used to construct gas sensors [134,135]. For insects, the outstanding selectivity and sensitivity of the olfactory system is achieved by filters that discriminate between small soluble binding proteins and odorant receptors.…”

Section: Gas Sensormentioning

confidence: 99%

“…Therefore, Liu et al designed an olfactory biosensor to immobilize OBPs from oriental fruit fly to detect the semiochemicals (Fig. 8B) [135]. After successful isolation and purification, OBPs were immobilized with a specially designed polyethylene glycol (PEG) SH-PEG-COOH linkage to generate a robust sensor membrane, which sensitively detected isoamyl acetate, beta-ionone, benzaldehyde, and other compounds.…”

Section: Gas Sensormentioning

confidence: 99%

Recent biomedical applications of bio-sourced materials

Elbaz

Gao

et al. 2018

Bio-des. Manuf.

View full text Add to dashboard Cite

Natural anisotropic nanostructures occurring in several organisms have gained more and more attention because of their obvious advantages in sensitivity, stability, security, miniaturization, portability, online use, and remote monitoring. Due to the development of research on nature-inspired bionic structures and the demand for highly efficient, low-cost microfabrication techniques, an understanding of and the ability to replicate the mechanism of structural coloration have become increasingly significant. These sophisticated structures have many unique functions and are used in many applications. Many sensors have been proposed based on their novel structures and unique optical properties. Several of these bio-inspired sensors have been used for infrared radiation/thermal, pH, and vapor techniques, among others, and have been discussed in detail, with an intense focus on several biomedical applications. However, many applications have yet to be discovered. In this review, we will describe these nanostructured materials based on their sources in nature and various structures, such as layered, hierarchical, and helical structures. In addition, we discuss the functions endowed by these structures, such as superhydrophobicity, adhesion, and high strength, enabling them to be employed in a number of applications in biomedical fields, including cell cultivation, biosensors, and tissue engineering.

show abstract

Investigating Electrochemical Stability and Reliability of Gold Electrode‐electrolyte Systems to Develop Bioelectronic Nose Using Insect Olfactory Receptor

et al. 2019

View full text Add to dashboard Cite

This article aims to demonstrate an electrochemically stable and reliable gold electrode‐electrolyte system to develop an insect odorant receptor (Drosophila melanogaster Or35a) based bioelectronic nose. Cyclic voltammograms (CVs) and electrochemical impedance spectroscopy (EIS) of bare gold electrodes, after modification with the self‐assembled monolayer (SAM) of 6‐mercaptohexanoic acid (MHA) and after immobilization with Or35a integrated into the lipid bilayers of liposomes were conducted in the presence of four different redox probes. Potassium ferri/ferrocyanide [Fe(CN)6]3−/[Fe (CN)6]4− and hydroquinone (H2Q) redox probes revealed variable and irreversible signals at the time scale of our measurements, with atomic force microscopy (AFM) images and x‐ray photoelectron spectroscopy (XPS) results suggesting gold surface etching due to the presence of CN− ions in case of [Fe(CN)6]3−/[Fe (CN)6]4−. Although the hexaammineruthenium complex showed stable electrochemical behaviour at all stages of biosensor development, changes in CV and EIS readings after each surface modifications were insignificant. PBS buffer as a non‐Faradaic medium, was found to provide reliable systems for electrochemical probing of modified gold electrodes with Or35a/liposomes in aqueous media. Using this system, we have shown that this novel biosensor can detect its known odorant E2‐hexenal selectively compared to methyl salicylate down to femtomolar concentration.

show abstract

Olfactory biosensor for insect semiochemicals analysis by impedance sensing of odorant-binding proteins on interdigitated electrodes

Cited by 65 publications

References 54 publications

Smell Sensors Based on Odorant Binding Proteins

Smell Sensors Based on Odorant Binding Proteins

Recent biomedical applications of bio-sourced materials

Investigating Electrochemical Stability and Reliability of Gold Electrode‐electrolyte Systems to Develop Bioelectronic Nose Using Insect Olfactory Receptor

Contact Info

Product

Resources

About