Oleifolioside B-mediated autophagy promotes apoptosis in A549 human non-small cell lung cancer cells

Abstract

The biochemical mechanisms of cell death by oleifolioside B (OB), a cycloartane-type triterpene glycoside isolated from Dendropanax morbifera Leveille, were investigated in A549 human lung carcinoma cells. Our data indicated that exposure to OB led to caspase activation and typical features of apoptosis; however, apoptotic cell death was not prevented by z-VAD-fmk, a pan-caspase inhibitor, demonstrating that OB-induced apoptosis was independent of caspase activation. Subsequently, we found that OB increased autophagy, as indicated by an increase in monodansylcadaverine fluorescent dye-labeled autophagosome formation and in the levels of the autophagic form of microtubule-associated protein 1 light chain 3 and Atg3, an autophagy-specific gene, which is associated with inhibiting phospho-nuclear factor erythroid 2-related factor 2 (Nrf2) expression. However, pretreatment with bafilomycin A1, an autophagy inhibitor, attenuated OB-induced apoptosis and dephosphorylation of Nrf2. The data suggest that OB-induced autophagy functions as a death mechanism in A549 cells and OB has potential as a novel anticancer agent capable of targeting apoptotic and autophagic cell death and the Nrf2 signaling pathway.

Inhibitory effect of 1-tetradecanol on Helicobacter pylori-induced production of interleukin-8 and vascular endothelial growth factor in gastric epithelial cells

“…No cytotoxic effects of 0-300 µM 1-TD on H. pylori-infected gastric epithelial cells were evident. In contrast, previous studies have demonstrated that oleifolioside A and B, ethanol and methanol extracts isolated from D. morbifera have cytotoxic effects on human leukemia cells (1), lung carcinoma cells (24), cervical carcinoma cells (4) and hepatocellular carcinoma cells (5). Therefore, the results of the present study suggested that the inhibition of H. pylori-induced inflammation in gastric epithelial cells is not due to cell toxicity.…”

Inhibition of mitochondrial autophagy protects donor lungs for lung transplantation against ischaemia‐reperfusion injury in rats via the mTOR pathway

“…24,25 Moreover, it was reported that PINK1 in conjunction with Parkin could affect mitochondrial integrity in some tissues, such as dopaminergic neurons. 30,31 Moreover, induction of mitochondrial autophagy was reported to serve as an inhibitor of cell proliferation. 27 Similarly, the PINK1/ Parkin-directed pathway was previously linked to mitochondrial damage, ubiquitylation and mitochondrial autophagy.…”

“…29 Both mitochondrial autophagy and apoptosis are highly regulated biological processes affecting tissue development and diseases, and a study reported that mitochondrial autophagy induced apoptosis in lung cancer cells. 30,31 Moreover, induction of mitochondrial autophagy was reported to serve as an inhibitor of cell proliferation. 32 Similarly, accumulating evidence has demonstrated that apoptosis is induced in ALI, and inhibition of apoptosis attenuates lung injury.…”

A map describing the association between effective components of traditional Chinese medicine and signaling pathways in cancer cells in vitro and in vivo

“…Alkaloids are a group of naturally occurring chemical 140 [114][115][116][117][118][119] pathway and down-regulation of inhibitor of apoptosis proteins (18). Another study reports that matrine inhibits the invasive activities of human osteosarcoma cells through down-regulation of the ERK/NF-κB pathway in intro and in vivo (19).…”

“…Fomitoside-K, a biologically active component isolated from a mushroom Fomitopsis nigra, also can increase apoptosis of YD-10B human oral squamous cells through the mitochondrial pathway (118). In A549 lung cancer cells, oleifolioside B, a cycloartane-type triterpene glycoside isolated from Dendropanax morbifera Leveille, promotes apoptosis through targeting the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway which can antagonize the NF-κB pathway (119). Periplocin and icariin which were mentioned above also belong to the glycoside family.…”