“…Furthermore, ROESY cross peaks at δ H 0.74 (3 H, s, H 3 ‐29)/5.48 (1H, d J = 9.3 Hz, H‐21) and 0.88 (3 H, s, H 3 ‐30)/4.03 (1H, H‐22) revealed the β ‐axial orientation of the H‐22, and the multiplicity as a doublet and the coupling constant of 9.3 Hz suggested an α ‐axial orientation of H‐21. This extensive 2D NMR analysis confirmed the identification of the aglycone of compound 2 as the already known sapogenol called R1‐barrigenol (olean‐12‐ene‐3 β ,15 α ,16 α ,21 β ,22 α ,28‐hexol), which is esterified at C‐21 by an acetyl function. In the 1 H NMR spectrum of compound 2 , signals of five anomeric protons were shown at δ H 5.06 (br s), 4.81 (d, J = 6.0 Hz), 4.72 (d, J = 7.3 Hz), 4.28 (d, J = 6.9 Hz) and 4.14 (d, J = 5.7 Hz), which correlated in the HSQC spectrum with five carbons at δ C 106.8, 101.9, 101.4, 103.6 and 104.6, respectively, indicating the presence of five sugar moieties.…”