Oleamide in Ipomoea and Dillenia Species and Inflammatory Activity Investigated through Ion Channel Inhibition

Abstract

Background: Oleamide is an essential substance for human health. So, the plants with high oleamide content are great sources for health care products. Objective: This study is conducted to investigate the quality of oleamide in plants and test the bioactivity in the selected two studied species. Methods: The three Ipomoea and five Dillenia species including Ipomoea alba, Ipomoea aquatica and Ipomoea pes-caprae, and Dillenia indica, Dillenia obovata, Dillenia ovata, Dillenia parviflora and Dillenia pentagyna were investigated for the quantity of oleamide by high-performance liquid chromatography. The biological activity test was conducted on the powder formulation of the chosen plants, Dillenia ovata and Dillenia parviflora at a ratio of 30:70, for anti-inflammatory activity ex vivo on a panel of molecular targets through ion channel inhibition including voltage-gated sodium channel, voltage-gated potassium channel, and the cardiac ion as human ether-a-go-go related gene. Results: The results showed that the leaf extracts of I. aquatica and D. ovata gave the highest and subsequent oleamide quantity following 7.52 and 5.17 mg/g. Out of the Dillenia formulation which contained various compounds, oleamide showed the highest percentages of inhibition at 8.0-20.0%, and 6.2-14.2% in voltage-gated sodium channel, and voltage-gated potassium channel which had slightly lower values than the oleamide standard, and no effect as 0.0% value inhibition in the cardiac ion channel. Conclusion: The Dillenia formulation exhibits anti-inflammatory activity without affecting the heart. Accordingly, the three studied Ipomoea and three studied Dillenia species may be used for the same activity as a single component or formulation with effective solvent for disease treatments.

Anti-inflammatory and detoxification activities of some Ipomoea species determined by ion channel inhibition and their phytochemical constituents

“…However, the experiment on oleamide activity merits further study given its use for treatments of Alzheimer's disease, stress, deep sleep, and appetite induction [10]. Recently, the anti-inflammatory effect of oleamide has been shown [11][12][13]. Additionally, phytol was present at 21.58% in I. batatas, 13.35% in I. carnea, and 11.76% in I. pes-caprae; but the amount was not high enough to functionally support the bioactivities of the plants.…”

In Vitro Propagation and Secondary Metabolite Production of Medicinal Plant of Euchresta horsfieldii (Lesch) Benn.

“…Hachisu et al (2015) explained that oleamide is a protective agent against memory loss in Alzheimer's disease and can increase drowsiness cause deep sleep, increase appetite, and has no side effects on blood pressure, heart rate, and body temperature. According to Ameamsri et al (2021), oleamide exhibits anti-inflammatory effects and has been used in the prevention and treatment of various diseases such as atherosclerosis, arthritis, and cancer through its metabolic conversion activity. According to Falowo et al (2017), siloxane compounds are compounds that have high antioxidant activity and play an active role as anti-microbials.…”

Comparison of Growth and Chemical Profile of Diatom Skeletonema grevillei in Bioreactor and Incubation-Shaking Cabinet in Two Growth Phases

“…It is found in green algae such as Chromochloris zofingiensis and in few terrestrial plants. There are numerous biological activities related to this compound [29][30][31]. In general, oleamide from green algae is used in the treatment/prevention of arthritis, atherosclerosis, thrombosis, and cancer [32].…”

“…In general, oleamide from green algae is used in the treatment/prevention of arthritis, atherosclerosis, thrombosis, and cancer [32]. It was reported that oleamide from terrestrial plants (Ipomoea and Dillenia species) excretes anti-inflammatory properties [29]. Furthermore, oleamide from the fungus Colletotrichum gloeosporioides has confirmed antimicrobial activity against Staphylococcus aureus (zone of inhibition = 25 mm) [33].…”