Okadaic acid increases fibronectin synthesis in MC353‐E1 cells

Choi, Je Yong; Ryoo, Hyun Mo; Lee, Byung Heon; Kim, In San; Park, Rang Woon; Sohn, Kun Young

doi:10.1080/15216549600201012

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…The concentration of OKA that we used in this experiment was determined to be optimal in our previous studies [Choi et al, 1995, 1996]. Northern blot analysis of mRNAs from several normal and tumor cells indicated that OP expression was consistently stimulated by OKA treatment regardless of cellular origin.…”

Section: Resultsmentioning

confidence: 98%

Okadaic acid stimulates osteopontin expression through de novo induction of AP‐1

Kim

Lee

Shin

et al. 2002

J of Cellular Biochemistry

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Osteopontin, a major non-collagenous bone matrix protein, is strikingly upregulated in various tissues under certain pathologic conditions, including cancer. However, the mechanism of upregulation of the osteopontin gene in tumor cells remains unclear. Okadaic acid, a strong non-phorbol ester tumor promoter, is known to stimulate the expression of osteopontin. The aim of the present study was to understand the mechanism by which okadaic acid regulates osteopontin gene expression. Okadaic acid stimulated osteopontin mRNA expression in several cell lines within 3 h, and the increase in osteopontin mRNA was sustained for 24 h. New protein synthesis was required for the okadaic acid-elicited increase in osteopontin mRNA expression. A serial promoter deletion study showed that the okadaic acid-response element is located between positions -265 and -73, a sequence that includes the Runx2, Ets-1, and AP-1 binding sequences. Okadaic acid increased the mRNA expression of AP-1 components but not of Runx2 or Ets-1. Site-directed mutagenesis and electrophoretic mobility shift assays confirmed that protein binding of the AP-1 consensus sequence is necessary for the okadaic acid-mediated osteopontin gene upregulation. These results indicate that de novo induction of the oncoprotein AP-1 is required for okadaic acid-stimulated osteopontin gene upregulation.

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Section: Resultsmentioning

confidence: 98%

Okadaic acid stimulates osteopontin expression through de novo induction of AP‐1

Kim

Lee

Shin

et al. 2002

J of Cellular Biochemistry

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Coordinate gene expression patterns during osteoblast maturation and retinoic acid treatment of MC3T3-E1 cells

Kitching

et al. 2002

Journal of Bone and Mineral Metabolism

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The clonal osteoblast-like cell line MC3T3-E1 undergoes time-dependent morphological changes leading to the formation of bone nodules in vitro. Serial analysis of gene expression was used to identify genes expressed in MC3T3-E1 cells, including known osteoblast markers, structural and matrix proteins, transcription factors, cell-cycle regulators, and housekeeping genes. Relative changes in the expression of 92 representative transcripts were determined by arrayed cDNA hybridization. Complex probes were derived from MC3T3-E1 cells during the proliferation, differentiation, and matrix mineralization stages, and from cells grown with all- trans-retinoic acid (RA), a potent bone morphogen. We found that the relative expression of 68 of these genes was higher during differentiation than in the earlier proliferative phase. In the mineralization phase, all but 16 cDNAs had lower normalized hybridization intensity ratios as compared with the differentiation phase. cDNAs for vimentin (Vim), presenilin 2 (Psen2), guanine nucleotide binding protein alpha stimulating (Gnas), gap-junction membrane channel protein beta 1 (Gjb1), fibroblast growth factor receptor 1 (Fgfr1), eukaryotic translation elongation factor 2 (Eef2), and calponin 2 (Cnn2) had higher normalized hybridization intensities in both differentiation and mineralization than in proliferation. RA treatment during the differentiation phase appeared to reduce the expression of the 92 genes examined, as 62 cDNAs had lower hybridization intensities with complex cDNA probes derived from RA-treated cells than with the probe from untreated cells. Several cDNAs representing genes with previously unrecognized RA responsiveness were identified by this comparison, including the receptor for bone morphogenetic proteins 2 and 4 (Bmp2/Bmp4) and hemoglobin Y.

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Okadaic acid increases fibronectin synthesis in MC353‐E1 cells

Cited by 2 publications

References 16 publications

Okadaic acid stimulates osteopontin expression through de novo induction of AP‐1

Okadaic acid stimulates osteopontin expression through de novo induction of AP‐1

Coordinate gene expression patterns during osteoblast maturation and retinoic acid treatment of MC3T3-E1 cells

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