Okadaic acid stimulates osteopontin expression through de novo induction of AP‐1

Kim, Hyun-Jung; Lee, Mi-Hye; Shin, Hong‐In; Choi, Je‐Yong; Ryoo, Hyun‐Mo

doi:10.1002/jcb.10280

Cited by 22 publications

(20 citation statements)

References 37 publications

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“…Moreover, as the OP promoter contains an AP1 binding site (Kim et al, 2002), it is likely that AP1 is involved in the regulation of OP gene transcription. To assess this involvement, ST2 cells were treated with FGF2 and their RNA extracts were then examined by Northern blotting for the expression of various fos-and jun-related mRNAs, namely, c-fos, FosB, All of the fos-and jun-related mRNAs we examined were up-regulated immediately after FGF2 treatment (Fig.…”

Section: Ap1 Components Are Induced By Fgf2 Resulting In Increased Amentioning

confidence: 99%

“…To determine whether FGF2-induced AP1 mRNA expression is translated into AP1 protein and that this could directly up-regulate OP gene expression, we performed an electrophoretic mobility shift assay (EMSA) using a radiolabeled oligonucleotide representing the AP1 binding site (TRE) in the proximal OP promoter (OP-TRE; Kim et al, 2002). For this purpose, MC3T3-E1 cells were cultured in the presence or absence of FGF2 for the indicated time periods and EMSA was performed on their nuclear extracts.…”

Section: Ap1 Components Are Induced By Fgf2 Resulting In Increased Amentioning

confidence: 99%

“…2A) by performing Northern blot analysis on RNA extracts with an OP cDNA probe (Kim et al, 2002). We found that FGF2 stimulated significantly OP gene expression all three cell types.…”

Section: Fgf2-induced Op Gene Expression Is Not An Immediate Responsementioning

confidence: 92%

“…This finding is due to an increase in the number of mature osteoblasts. AP1 binding sites are also present in the promoters of many developmentally regulated osteoblast marker genes, including collagenase, osteocalcin (OC), and osteopontin (OP) (McCabe et al, 1996;D'Alonzo et al, 2002;Kim et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Erk pathway and activator protein 1 play crucial roles in FGF2‐stimulated premature cranial suture closure

Kim

Lee

Park

et al. 2003

Developmental Dynamics

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Cranial sutures are an important growth center of the cranial bones, and the suture space must be maintained to permit the cranial adjustments needed to accommodate brain growth. Craniosynostosis, characterized by premature suture closure, mainly results from mutations that generate constitutively active fibroblast growth factor (FGF) receptors. FGF signaling, thus, is responsible for the pathogenesis of craniosynostosis. Even though FGF activates many different signaling pathways, the one involved in premature suture closure has not been defined. We observed that placing FGF2-soaked bead on the osteogenic fronts of cultured mouse calvaria accelerates cranial suture closure and strongly induces the expression of osteopontin, an early marker of differentiated osteoblasts. FGF2 treatment also induced fos and jun mRNAs and later increased the nuclear levels of activator protein 1 (AP1).

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Section: Ap1 Components Are Induced By Fgf2 Resulting In Increased Amentioning

confidence: 99%

Section: Ap1 Components Are Induced By Fgf2 Resulting In Increased Amentioning

confidence: 99%

“…2A) by performing Northern blot analysis on RNA extracts with an OP cDNA probe (Kim et al, 2002). We found that FGF2 stimulated significantly OP gene expression all three cell types.…”

Section: Fgf2-induced Op Gene Expression Is Not An Immediate Responsementioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Erk pathway and activator protein 1 play crucial roles in FGF2‐stimulated premature cranial suture closure

Kim

Lee

Park

et al. 2003

Developmental Dynamics

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“…10 Its transcription can be activated by various stimuli through AP1 [11][12][13] and RUNX2, 14 and both of these factors could interact with SP1 in a cooperative manner.…”

Section: Introductionmentioning

confidence: 99%

SPP1 promoter polymorphisms and glioma risk in a Chinese Han population

Chen

et al. 2010

J Hum Genet

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SPP1 was found to be significantly upregulated in many kinds of malignant tumors, including gliomas. Considering that gene polymorphisms have been implicated in the development of gliomas, we performed an association study between SPP1 functional promoter region polymorphisms and glioma risk in a Chinese population. We found significant evidence of an association between SPP1 promoter polymorphisms and glioma risk. For the À155_156insG variant, the À155_156GG allele was found to be significantly associated with an increased risk of glioma (P¼0.020, odds ratio (OR)¼1.202, 95% confidence interval (CI): 1.028-1.408). Individuals with the genotype containing the GG allele had a 1.372-fold increased risk (P¼0.006, OR¼1.372, 95% CI: 1.095-1.719). Further stratified analyses suggested that a significant association existed in adult glioma patients, male subjects and in cases without a family history of cancer. Alternatively, the study of single-nucleotide polymorphism À443C/T in a recessive model revealed that the genotype CC + CT significantly decreased the risk of glioma when compared with TT (P¼0.023, OR¼0.774, 95% CI: 0.621-0.966). After the analysis of haplotypes, the haplotype À155_156GG/À443T was represented at a significantly higher frequency in cases (P¼0.029, OR¼1.192, 95% CI: 1.018-1.395). Cellular assay indicated that the transcriptional activity of the SPP1 promoter containing the À155_156GG allele significantly increased in glioma cells. Thus, variants of the SPP1 promoter might influence the risk of glioma by regulating promoter activity. Further analyses are necessary to validate our observation in larger samples or in other ethnic groups.

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Establishment and characterization of a stable cell line to evaluate cellular Runx2 activity

Kim¹,

Park²,

Kim³

et al. 2004

J of Cellular Biochemistry

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Runx2 is an essential transcription factor for osteoblast differentiation from early commitment step to final differentiation. Based on its crucial role in osteoblast differentiation, the transcriptional activity of Runx2 protein implies more valuable information for osteoblast differentiation than any other parameters, such as Runx2 mRNA or protein level. Thus, a sensitive, specific, and consistent method to determine the Runx2 transcriptional activity has long been expected. Here we suggest a stable cell line that carries 6xOSE2-Luciferase reporter vector would be a good evaluation system to determine biological Runx2 transcriptional activity. The proliferation rate, cell shape, and the myogenic differentiation potential of the cloned cell line were similar to those of parental premyoblastic C2C12 cells. The cells specifically responded to Runx2 modulating agent such as FGF2. The stable cell line responded 5-6 folds more sensitively than the transiently transfected cells with Runx2. Though overexpression of any Runx gene stimulated the luciferase activity, Runx2 enhanced the reporter activity the highest. Collectively, the 6xOSE2-luc stable cells would be a good biological evaluation system to assess the activity of extracellular Runx2 modulating stimulations as well as the signal transduction pathways involved in the stimulations.

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Okadaic acid stimulates osteopontin expression through de novo induction of AP‐1

Cited by 22 publications

References 37 publications

Erk pathway and activator protein 1 play crucial roles in FGF2‐stimulated premature cranial suture closure

Erk pathway and activator protein 1 play crucial roles in FGF2‐stimulated premature cranial suture closure

SPP1 promoter polymorphisms and glioma risk in a Chinese Han population

Establishment and characterization of a stable cell line to evaluate cellular Runx2 activity

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