Okadaic acid activates Wnt/β-catenin-signaling in human HepaRG cells

Dietrich, Jessica; Sommersdorf, Cornelia; Gohlke, Svenja; Poetz, Oliver; Traenkle, Bjoern; Rothbauer, Ulrich; Hessel-Pras, Stefanie; Lampen, Alfonso; Braeuning, Albert

doi:10.1007/s00204-019-02489-4

Cited by 10 publications

(5 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dinophysis belongs to the HAB-forming group because it contains toxins that can cause diarrhetic shellfish poisoning (DSP) if humans consume contaminated shellfish (Taylor et al 2013). According to (Dietrich et al 2019), the symptoms of poisoning caused by Dinophysis (DSP) include diarrhea induced by okadaic acid. Gymnodinium is a type of HABforming phytoplankton that causes red tides and oxygen depletion (Pitcher and Probyn 2016).…”

Section: Discussionmentioning

confidence: 99%

Detection of marine microalgae (phytoplankton) quality to support seafood health: A case study on the west coast of South Sulawesi, Indonesia

et al. 2021

View full text Add to dashboard Cite

Abstract. Tambaru R, Burhanuddin AI, Massinai A, Amran MA. 2021. Detection of marine microalgae (phytoplankton) quality to support seafood health: a case study on the west coast of South Sulawesi, Indonesia. Biodiversitas 22: 5179- 5186. The research aimed to detect marine microalgae quality to support seafood health was carried out from January to November 2020 along the west coast of South Sulawesi, Indonesia. Samples were collected from the coastal waters of Pangkep District, Maros District, and the northern part of Makassar City. Phytoplankton cell counts were obtained using the deposition method developed by Uthermol. Phytoplankton cell abundances were calculated through sweeping (census) using a Sedgwick Rafter Cell (SRC). Two-way analysis of variance (ANOVA) was used to compare the distribution of marine microalgae community abundance between observation stations and periods. Based on the types and relative abundance of phytoplankton present, i.e., harmful algal bloom (HAB) forming or not (non-HAB), the results showed the quality of marine microalgae, specifically, phytoplankton was relatively good. Many more non-HAB (94-98%) than HAB (2-6%) marine microalgae were detected. Thus, the phytoplankton flourishing in these waters is mostly suitable as food for other organisms, including fish and shellfish. This also means that if fishers harvest these fish and shellfish, they should be fit and safe for human consumption.

show abstract

Section: Discussionmentioning

confidence: 99%

Detection of marine microalgae (phytoplankton) quality to support seafood health: A case study on the west coast of South Sulawesi, Indonesia

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Thus, details of S phase progression and endogenous DNA replication were revealed with a PCNA-Cb (Burgess et al, 2012), which also enabled high-throughput screening for cell-cycleaffecting drugs (Schorpp et al, 2016). Likewise, a Cb against hypophosphorylated β-catenin (BC1-Cb) was used successfully to monitor its nuclear translocation, which is required for signal transduction in the Wnt/β-catenin signaling pathway (Dietrich et al, 2019;Traenkle et al, 2015). For proper imaging, Cbs must not alter the intrinsic function, localization or dynamics of their antigens.…”

Section: Intracellular Applications Of Nbs Chromobodies For Live-cell...mentioning

confidence: 99%

30 years of nanobodies – an ongoing success story of small binders in biological research

Frecot,

Froehlich,

Rothbauer

2023

Journal of Cell Science

Self Cite

View full text Add to dashboard Cite

A milestone in the field of recombinant binding molecules was achieved 30 years ago with the discovery of single-domain antibodies from which antigen-binding variable domains, better known as nanobodies (Nbs), can be derived. Being only one tenth the size of conventional antibodies, Nbs feature high affinity and specificity, while being highly stable and soluble. In addition, they display accessibility to cryptic sites, low off-target accumulation and deep tissue penetration. Efficient selection methods, such as (semi-)synthetic/naïve or immunized cDNA libraries and display technologies, have facilitated the isolation of Nbs against diverse targets, and their single-gene format enables easy functionalization and high-yield production. This Review highlights recent advances in Nb applications in various areas of biological research, including structural biology, proteomics and high-resolution and in vivo imaging. In addition, we provide insights into intracellular applications of Nbs, such as live-cell imaging, biosensors and targeted protein degradation.

show abstract

“…The results shade some light on OA carcinogenic mechanisms. β-catenin (CTNNB1), a key member of the pathway, was affected at the transcription and post-transcription levels and cellular location [ 60 ].…”

Section: Human Toxicology Of Dstsmentioning

confidence: 99%

OMICs Approaches in Diarrhetic Shellfish Toxins Research

Campos

Freitas

Almeida

et al. 2020

Toxins

View full text Add to dashboard Cite

Diarrhetic shellfish toxins (DSTs) are among the most prevalent marine toxins in Europe’s and in other temperate coastal regions. These toxins are produced by several dinoflagellate species; however, the contamination of the marine trophic chain is often attributed to species of the genus Dinophysis. This group of toxins, constituted by okadaic acid (OA) and analogous molecules (dinophysistoxins, DTXs), are highly harmful to humans, causing severe poisoning symptoms caused by the ingestion of contaminated seafood. Knowledge on the mode of action and toxicology of OA and the chemical characterization and accumulation of DSTs in seafood species (bivalves, gastropods and crustaceans) has significantly contributed to understand the impacts of these toxins in humans. Considerable information is however missing, particularly at the molecular and metabolic levels involving toxin uptake, distribution, compartmentalization and biotransformation and the interaction of DSTs with aquatic organisms. Recent contributions to the knowledge of DSTs arise from transcriptomics and proteomics research. Indeed, OMICs constitute a research field dedicated to the systematic analysis on the organisms’ metabolisms. The methodologies used in OMICs are also highly effective to identify critical metabolic pathways affecting the physiology of the organisms. In this review, we analyze the main contributions provided so far by OMICs to DSTs research and discuss the prospects of OMICs with regard to the DSTs toxicology and the significance of these toxins to public health, food safety and aquaculture.

show abstract

Okadaic acid activates Wnt/β-catenin-signaling in human HepaRG cells

Cited by 10 publications

References 55 publications

Detection of marine microalgae (phytoplankton) quality to support seafood health: A case study on the west coast of South Sulawesi, Indonesia

Detection of marine microalgae (phytoplankton) quality to support seafood health: A case study on the west coast of South Sulawesi, Indonesia

30 years of nanobodies – an ongoing success story of small binders in biological research

OMICs Approaches in Diarrhetic Shellfish Toxins Research

Contact Info

Product

Resources

About