Oilseed rape — allergen or irritant?

Galloway,

doi:10.1046/j.1365-2222.2000.00844.x

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…Since 1954, several reports have examined the question of the potential role of oilseed rape (Brassica napus) in pollen allergy [1, 2, 3, 4, 5, 6, 7]. Some of them concluded that type I hypersensitivity to rape pollen is limited to atopic patients with a history of pollen allergy [2, 3].…”

Section: Introductionmentioning

confidence: 99%

Characterization of High-Molecular-Mass Allergens in Oilseed Rape Pollen

Chardin

Mayer

Sénéchal

et al. 2001

Int Arch Allergy Immunol

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Background:Oilseed rape pollen allergies have been previously described as the result of cross-sensitization with various pollens. Recently, several proteins have been identified as oilseed rape allergens. The aim of the present work was the characterization of oilseed rape pollen allergens by two-dimensional (2-D) gel analysis and amino acid microsequencing. Methods: Water extractable proteins from oilseed rape pollen were separated by isoelectrofocusing and then transferred onto a nitrocellulose sheet. Twenty-one human sera from pollen- or mustard-allergic individuals were screened for their reactivity to oilseed rape proteins. Eleven sera possessed IgE which recognized oilseed rape pollen proteins and one serum was selected for further 2-D characterization and amino acid microsequencing of the allergens. Results: The results showed that three molecules from oilseed rape pollen were identified as oilseed rape allergens which have not yet been described. These three proteins were molecules of 70 kD with a pI >8, 40 kD with a pI around 10 and 80 kD with a pI around 5. These proteins displayed identities with the berberine bridge protein, a receptor-like protein kinase and the cobalamin-independent methionine synthetase from Arabidopsis thaliana, respectively. The genes encoding the putative Arabidopsis molecules are located on chromosome 1 (berberine bridge protein) and chromosomes 3 and 4 (receptor-like protein kinases). Conclusion: These results show that certain high-molecular-mass proteins from oilseed rape pollen are allergens.

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Section: Introductionmentioning

confidence: 99%

Characterization of High-Molecular-Mass Allergens in Oilseed Rape Pollen

Chardin

Mayer

Sénéchal

et al. 2001

Int Arch Allergy Immunol

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“…Possible adverse effects caused by oilseed rape cultivation have been suggested since many years, especially in Britain, and several reports discuss the scientific basis of such effects (1–5). These adverse effects have been mainly attributed to volatile organic compounds that may be involved in the irritancy and/or in the allergenicity of rapeseed proteins (6).…”

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confidence: 99%

Polygalacturonase (pectinase), a new oilseed rape allergen

Chardin¹,

Mayer²,

Sénéchal³

et al. 2003

Allergy

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“…Finally, some patients, convinced that oilseed rape is causing their symptoms despite negative skin prick allergy tests, may be prone to the potential irritant effect of oilseed rape as opposed the its allergenic effect 19 …”

Section: Discussionmentioning

confidence: 99%