Oil-degrading bacteria from a membrane bioreactor (BF-MBR) system for treatment of saline oily waste: Isolation, identification and characterization of the biotechnological potential

Cappello, Simone; Volta, Anna; Santisi, Santina; Morici, Claudia; Mancini, Giuseppe; Quatrini, Paola; Genovese, Maria Inês; Yakimov, Michail M.; Torregrossa, Michele

doi:10.1016/j.ibiod.2015.12.028

Cited by 53 publications

(27 citation statements)

References 57 publications

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“…Hydrocarbonoclastic bacteria such as Alcanivorax and Marinobacter are well known for their ability to degrade hydrocarbons alone with a high rate (Cappello et al . ) but a single species can metabolize only a limited range of hydrocarbon substrates because it does not have all the enzymatic machinery to degrade a pollutant until its complete mineralization (Mahjoubi et al . ).…”

Section: Discussionmentioning

confidence: 99%

“…Alkanes are the major constituents of crude oil and the excellent ability of Alcanivorax to degrade branched alkanes is one of the factors that allow this strain to predominate in oil-contaminated seawater (Hara et al 2003). Hydrocarbonoclastic bacteria such as Alcanivorax and Marinobacter are well known for their ability to degrade hydrocarbons alone with a high rate (Cappello et al 2016) but a single species can metabolize only a limited range of hydrocarbon substrates because it does not have all the enzymatic machinery to degrade a pollutant until its complete mineralization (Mahjoubi et al 2013). For this reason, a consortium of many different bacterial species, with broad enzymatic capacities, is usually involved in oil degradation (R€ oling et al 2002;De Pasquale et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…The following step was to apply 10 ll of bacterial culture on the oil surface. The diameter of clear zone around the bacterial suspension was measured as the activity of surfactants (Cappello et al 2016). The emulsification index (E24) of different bacterial culture was determined by adding 2 ml of hexadecane to the same amount of bacterial culture.…”

Section: Biosurfactant Production and Emulsification Testmentioning

confidence: 99%

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Isolation, characterization and determination of biotechnological potential of oil-degrading bacteria from Algerian centre coast

et al. 2019

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Aims The Algerian coastline is exposed to several types of pollution, including hydrocarbons. The aim of this work was to isolate oil‐degrading bacteria and to explore the intrinsic bioremediation potential of part of its contaminated harbour. Methods and results A collection of 119 strains, capable to grow on mineral medium supplemented with hydrocarbons, were obtained from polluted sediment and seawater collected from Sidi Fredj harbour (Algiers). Twenty‐three strains were selected for further studies. Sequencing of the 16S rRNA gene showed that most isolates belong to genera of hydrocarbonoclastic bacteria (Alcanivorax), generalist hydrocarbons degraders (Marinobacter, Pseudomonas, Gordonia, Halomonas, Erythrobacter and Brevibacterium) and other bacteria not known as hydrocarbon degraders (Xanthomarina) but were able to degrade hydrocarbons. Strains related to Marinobacter and Alcanivorax were frequently isolated from our samples and resulted the most effective in degrading crude oil. Screening of catabolic genes alkB and xylA revealed the presence of alkB gene in several bacterial strains; one isolate harboured both catabolic genes while other isolates carried none of the studied genes. However, they grew in the presence of crude oil implying the existence of other biodegradation pathways. Conclusions The samples of seawater and sediment from the Algerian coast contain high level of hydrocarbon‐degrading bacteria that could be interesting and useful for future bioremediation purposes. Significance and Impact of the Study This investigation demonstrates the diversity of hydrocarbon‐degrading bacteria from a marine‐contaminated area in Algeria, and their variable biodegradation abilities

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Biosurfactant Production and Emulsification Testmentioning

confidence: 99%

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Isolation, characterization and determination of biotechnological potential of oil-degrading bacteria from Algerian centre coast

et al. 2019

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“…Immobilization minimizes cell losses and assures high cell loading without negative effects typical for the concentrated biomass, such as metabolic activity decrease and cell death. In comparison with suspended cells (SC), IC are protected from elimination by predatory protists, they are slow-growing and stress-resistant, have conventional storage and transportation form, high plasmid stability, and often increased catalytic activity [13,[67][68][69][70][71][72][73][74][75]. Immobilization carriers can partly (ad)sorb the pollutant molecules, resulting in the decrease of their concentration and toxic impact.…”

Section: Advantages Of Immobilized Cellsmentioning

confidence: 99%

Advanced Rhodococcus Biocatalysts for Environmental Biotechnologies

2019

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The review is devoted to biocatalysts based on actinobacteria of the genus Rhodococcus, which are promising for environmental biotechnologies. In the review, biotechnological advantages of Rhodococcus bacteria are evaluated, approaches used to develop robust and efficient biocatalysts are discussed, and their relevant applications are given. We focus on Rhodococcus cell immobilization in detail (methods of immobilization, criteria for strains and carriers, and optimization of process parameters) as the most efficient approach for stabilizing biocatalysts. It is shown that advanced Rhodococcus biocatalysts with improved working characteristics, enhanced stress tolerance, high catalytic activities, human and environment friendly, and commercially viable are developed, which are suitable for wastewater treatment, bioremediation, and biofuel production.

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“…The most commonly used remediation methods are chemical oxidation and enhanced oxidation processes [107][108][109], bioremediation processes [110][111][112], solid-phase processes [113][114][115][116], Bioslurry processes [117], and incinerations [118][119][120][121]. Table 2 provides a comparison between various oily wastewater treatment techniques [10,53].…”

Section: Freeze/thaw Processmentioning

confidence: 99%