Isolation, characterization and determination of biotechnological potential of oil-degrading bacteria from Algerian centre coast

Abstract

Aims The Algerian coastline is exposed to several types of pollution, including hydrocarbons. The aim of this work was to isolate oil‐degrading bacteria and to explore the intrinsic bioremediation potential of part of its contaminated harbour. Methods and results A collection of 119 strains, capable to grow on mineral medium supplemented with hydrocarbons, were obtained from polluted sediment and seawater collected from Sidi Fredj harbour (Algiers). Twenty‐three strains were selected for further studies. Sequencing of the 16S rRNA gene showed that most isolates belong to genera of hydrocarbonoclastic bacteria (Alcanivorax), generalist hydrocarbons degraders (Marinobacter, Pseudomonas, Gordonia, Halomonas, Erythrobacter and Brevibacterium) and other bacteria not known as hydrocarbon degraders (Xanthomarina) but were able to degrade hydrocarbons. Strains related to Marinobacter and Alcanivorax were frequently isolated from our samples and resulted the most effective in degrading crude oil. Screening of catabolic genes alkB and xylA revealed the presence of alkB gene in several bacterial strains; one isolate harboured both catabolic genes while other isolates carried none of the studied genes. However, they grew in the presence of crude oil implying the existence of other biodegradation pathways. Conclusions The samples of seawater and sediment from the Algerian coast contain high level of hydrocarbon‐degrading bacteria that could be interesting and useful for future bioremediation purposes. Significance and Impact of the Study This investigation demonstrates the diversity of hydrocarbon‐degrading bacteria from a marine‐contaminated area in Algeria, and their variable biodegradation abilities

Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria

“…Many groups of microorganisms have been reported to play a role in hydrocarbons degradation in the marine environment, such as the filamentous fungi genera Aspergillus and Penicillium [9] and the yeast genus Candida [10], nonetheless, bacteria are considered the major intervenient in hydrocarbons biodegradation at sea [11]. Members of the classes Gammaproteobacteria, Alphaproteobacteria and Actinobacteria have been isolated from seawater and marine sediment samples showing hydrocarbon bioremediation potential [12][13][14]. When considering the application of bioaugmentation techniques to tackle oil spills, the use of a consortium of microorganisms may be more effective than using single bacterial strains, since different groups of bacteria can metabolize different groups of hydrocarbons [15,16], by producing different oil-degrading enzymes [17] and biosurfactants [18].…”

Untapped Potential of Moving Bed Biofilm Reactors with Different Biocarrier Types for Bilge Water Treatment: A Laboratory-Scale Study

“…Denaro et al [50] examined the biofilm formed in polyethylene terephthalate (PET) biofilms, and Prolixibacteraceae was identified as the most abundant within the Bacteroidetes. Djahnit et al [51] found that isolated Xanthomarina gelatinilytica can degrade 17% of crude oil after 1 week of incubation. Yao et al [52] found Xanthomarina as one of the more dominant genera in aerobic granular sludge treating hypersaline wastewater.…”

Antibiotic Resistance Profile of Escherichia coli Isolated from Bovine Subclinical Mastitis of Dairy Farms in Algeria from 2017 to 2019

“…The forward and reverse primers used for PCR were bla-CTX-M-F 5´-ACCGCCGATAATTCGCAGAT-3´ and bla-CTX-M-R 5´-GATATCGTTGGTGGTGCCATA -3, respectively (Tabar et al, 2016). Briefly, 1 ml of an overnight E. coli culture in BHI (Bio-Rad, France) was used to prepare DNA by the boiling method (Djahnit et al, 2018). To amplify the CTX-M gene by PCR, 5 µl of the DNA was used.…”