Oil and protein quality of groundnut mutants

Sharma, Nimisha; Mehta, S. L.; Patil, S. H.; Eggum, B. O.

doi:10.1007/bf01093892

Cited by 9 publications

(7 citation statements)

References 7 publications

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“…1997). Earlier studies on induced mutagenesis in groundnut reported an increase in O/L ratio to 1.65 using gamma rays (Badigannavar 2007), 3.3 using X rays (Sharma et al. 1981) and to 3.5 using ethyl methane sulfonate (Dwivedi et al.…”

Section: Discussionmentioning

confidence: 99%

“…Besides oils with higher O/L ratio are good for lowering the risk of cardiovascular disease (OÕByrne et al 1997). Earlier studies on induced mutagenesis in groundnut reported an increase in O/L ratio to 1.65 using gamma rays (Badigannavar 2007), 3.3 using X rays (Sharma et al 1981) and to 3.5 using ethyl methane sulfonate (Dwivedi et al 1998). Isolation of high oleate mutant (TGM 192M) with 5.5 O/L ratio is of first of its kind in Spanish (var.…”

Section: Discussionmentioning

confidence: 99%

“…Looking in to the benefits of high O/L trait in groundnut, researchers had tried to induce high oleic acid through mutagenesis. Considerable improvement for oleic acid content in groundnut mutants with the treatment of X rays, EMS or gamma rays was observed (Sharma et al. 1981, Dwivedi et al.…”

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confidence: 99%

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Induced variability for fatty acid profile and molecular characterization of high oleate mutant in cultivated groundnut (Arachis hypogaea L.)

2011

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Groundnut is an important oilseed crop grown extensively in tropical and subtropical regions in nearly 100 different countries. Monounsaturated fatty acid like oleic acid is known to have beneficial health effects. Hence, recent research efforts are directed towards an improvement of oleic acid in groundnut genotype, TFDRG 5. In the present study, 47 true breeding mutants with various morphological traits were isolated at M 3 through gamma rays and/or sodium azide mutagenesis. These mutants were characterized for oil and fatty acid compositions at M 8 and M 9 generations. Of them, TGM 192M had nearly 27.3% improvement in oleic acid content which in turn increased the oleic/linoleic acid (O/L) ratio to 5.5 from 2.0 in parent. Sequencing of mutated ahFAD2A gene detected several point mutations in the coding region. Restriction digestion with Hpy99I and in silico translation of the mutated sequence further confirmed the G to A transition is responsible for high oleate trait in this mutant seed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Induced variability for fatty acid profile and molecular characterization of high oleate mutant in cultivated groundnut (Arachis hypogaea L.)

2011

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“…13.13 Estimation of Fatty Acids by Gas-Liquid Chromatography (GLC) Morrison and Smith (1964); Sharma et al (1981) Analysis of complex fatty acid mixtures can be carried out by GLC. In this procedure the fatty acids are first converted into a volatile form, usually their methyl esters.…”

Section: Principlementioning

confidence: 99%

Analytical Techniques in Biochemistry and Molecular Biology

Katoch

2011

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“…The mixture was again heated for 2 minutes and methylesters were separated using n-hexane. Fatty acid composition of these methylesters was determined by gas chromatography using SHIMADZU, GC-4, BPTF Model Gas chromatograph equipped with a flame ionization detector (SHARMA et al 1981).…”

Section: Fatty Acid Estimationmentioning

confidence: 99%

Effect of Macrophomina phaseolina Infection on the Physico‐Chemical Components of Groundnut Seed

Sharma

Bhowmik

1987

Journal of Phytopathology

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Various aspects of groundnut seed deterioration due to M. phaseolina infection were studied. Fungal infection caused both quantitative and qualitative damage to the seed. It resulted in characteristic discoloration of pod and kernel. There was marked reduction in the pod and kernel yield, shelling percentage and oil content. The oil extracted from healthy kernels was barium yellow, whereas, oil from diseased kernels was amber yellow in color. The diseased kernels contained lesser amounts ofstearic and behenic acids but higher quantities of palmitic, oleic, linoleic and arachidic acids. Four fatty acids, namely, lauric, myristic, palmitoleic and eicosenoic acids were absent in diseased kernels.

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Oil and protein quality of groundnut mutants

Cited by 9 publications

References 7 publications

Induced variability for fatty acid profile and molecular characterization of high oleate mutant in cultivated groundnut (Arachis hypogaea L.)

Induced variability for fatty acid profile and molecular characterization of high oleate mutant in cultivated groundnut (Arachis hypogaea L.)

Analytical Techniques in Biochemistry and Molecular Biology

Effect of Macrophomina phaseolina Infection on the Physico‐Chemical Components of Groundnut Seed

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