Ohtaekwangia koreensis gen. nov., sp. nov. and Ohtaekwangia kribbensis sp. nov., isolated from marine sand, deep-branching members of the phylum Bacteroidetes

Abstract

Two Gram-stain-negative, non-motile, non-spore-forming, rod-shaped bacterial strains, designated 3B-2T and 10AOT, were isolated from a sand sample collected from the west coast of the Korean peninsula by using low-nutrient media, and their taxonomic positions were investigated in a polyphasic study. The strains did not grow on marine agar. They grew optimally at 30 °C and pH 6.5–7.5. Strains 3B-2T and 10AOT shared 97.5 % 16S rRNA gene sequence similarity and mean level of DNA–DNA relatedness of 12 %. In phylogenetic trees based on 16S rRNA gene sequences, strains 3B-2T and 10AOT, together with several uncultured bacterial clones, formed independent lineages within the evolutionary radiation encompassed by the phylum Bacteroidetes. Strains 3B-2T and 10AOT contained MK-7 as the predominant menaquinone and iso-C15 : 0 and C16 : 1ω5c as the major fatty acids. The DNA G+C contents of strains 3B-2T and 10AOT were 42.8 and 44.6 mol%, respectively. Strains 3B-2T and 10AOT exhibited very low levels of 16S rRNA gene sequence similarity (<85.0 %) to the type strains of recognized bacterial species. These data were sufficient to support the proposal that the novel strains should be differentiated from previously known genera of the phylum Bacteroidetes. On the basis of the data presented, we suggest that strains 3B-2T and 10AOT represent two distinct novel species of a new genus, for which the names Ohtaekwangia koreensis gen. nov., sp. nov. (the type species; type strain 3B-2T = KCTC 23018T = CCUG 58939T) and Ohtaekwangia kribbensis sp. nov. (type strain 10AOT = KCTC 23019T = CCUG 58938T) are proposed.

Planktonic and sedimentary bacterial diversity of Lake Sayram in summer

“… 2011 ; Yoon et al. 2011 ; Roeselers and Newton 2012 ). The genus Desulfohalobium inhabited in hypersaline lakes (Ollivier et al.…”

Diversion and phylogenetic relatedness of filterable bacteria from Norwegian tap and bottled waters

“…Subsequently an isolate predicted to belong to the same genus was isolated from filtered lake water (Maejima et al 2018). The members of the genus seen unremarkable with respect to their cellular properties (Yoon et al 2011), and at present too little information is available to judge the importance of the current findings.…”

“…The type-strain was isolated from a sand sample collected from the west coast of the Korean peninsula along with a O. kribbensis to which it shared 97.5% 16S rDNA gene sequence identity. A new genus was proposed to house these species (Yoon et al 2011). Subsequently an isolate predicted to belong to the same genus was isolated from filtered lake water (Maejima et al 2018).…”

Effects of ultrasonic pretreatment on quantity and composition of bacterial DNA recovered from granular activated carbon used for drinking water treatment

“…[32,33] Its functions and ecological roles have not been fully studied yet. Although each community had similar dominant members, ultrasonication recovered some minor members that were missed when ultrasonication was not applied.…”