“Off-the-shelf” microfluidic devices for the production of liposomes for drug delivery

Bottaro, Elisabetta; Nastruzzi, Claudio

doi:10.1016/j.msec.2016.03.056

Cited by 28 publications

(21 citation statements)

References 15 publications

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“…Interestingly, it is worth mentioning that DOPC5 prepared using SHM were consistently smaller in size with lower dispersity (~0.1) than the ones prepared using "off-the-shelf" microfluidic devices: T-junction and X-junction (Bottaro and Nastruzzi, 2016;Jeffs et al, 2005;Kulkarni et al, 2017). Furthermore, SHM liposomes were more responsive to the change in FRR and less susceptible to the change in TFR compared to the junction devices, and smaller and more uniform liposomes were produced ( Figure S7).…”

Section: [Tfr/frr Effect]mentioning

confidence: 97%

Sterically stabilized liposomes production using staggered herringbone micromixer: Effect of lipid composition and PEG-lipid content

Cheung

Al-Jamal

2019

International Journal of Pharmaceutics

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Preparation of lipid-based drug delivery systems by microfluidics has been increasingly popular, due to the reproducible, continuous and scalable nature of the microfluidic process. Despite exciting development in the field, versatility and superiority of microfluidics over conventional methods still need further evidence, since preparing clinically-relevant sterically stabilised liposomes has been lacking. The present study describes the optimisation of PEGylated liposomal formulations of various rigidity using staggered herringbone micromixer (SHM). The effect of both processing parameters (total flow rate (TFR) and aqueous-to-ethanol flow rate ratio (FRR)) and formulation parameters (lipid components and composition, initial lipid concentration and aqueous media) was investigated and discussed. Liposomal formulations consist of 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC), 1,2dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or 1,2-distearoyl-sn-glycero-3phosphatidylcholine (DSPC), with cholesterol and PEGylated lipid (DSPE-PEG2000) were successfully prepared with the desired size (~100 nm) and dispersity (< 0.2). Doxorubicin was successfully encapsulated in these liposomes at high (> 80%) encapsulation efficiency using the pH-gradient remote loading method, illustrating their bilayer integrity and capability as drug delivery systems. We demonstrated that clinically-relevant PEGylated liposomal formulations could be prepared with properties comparable to conventional techniques. Limitations and recommendations on the microfluidic production of PEGylated liposomes were also discussed.

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Section: [Tfr/frr Effect]mentioning

confidence: 97%

Sterically stabilized liposomes production using staggered herringbone micromixer: Effect of lipid composition and PEG-lipid content

Cheung

Al-Jamal

2019

International Journal of Pharmaceutics

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“…In addition, nanoliposomes have the advantages of nanoparticles, which can improve targeting and absorption by intestinal epithelial cells. Therefore, nanoliposomes can be used as potential carriers in a food system [ 17 , 18 , 19 , 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Conditions for Cyanidin-3-OGlucoside (C3G) Nanoliposome Production by Response Surface Methodology and Cellular Uptake Studies in Caco-2 Cells

et al. 2017

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We aimed to optimize the formulation of C3G nanoliposomes using response surface methodology. Additionally, we evaluated the stability, particle change, and encapsulation efficiency (EE) of C3G nanoliposomes under different temperatures and storage durations, as well as in simulated gastrointestinal juice (SGF) and simulated intestinal fluid. The morphology of C3G nanoliposomes was observed by transmission electron microscope. The ability of C3G nanoliposomes to affect cancer cell morphology and inhibit cancer cell proliferation was studied with Caco-2 cells. Reverse-phase evaporation method is a simple and efficient method for liposome preparation. The optimal preparation conditions for this method were as follows: C3G concentration of 0.17 mg/mL, phosphatidylcholine/cholesterol ratio of 2.87, and rotary evaporation temperature of 41.41 °C. At optimal conditions, the particle size and EE of the C3G nanoliposomes were 165.78 ± 4.3 nm and 70.43% ± 1.95%, respectively. The C3G nanoliposomes showed an acceptable stability in SGF at 37 °C for 4 h, but were unstable under extended storage durations and high temperatures. Moreover, our results showed that different concentrations of C3G nanoliposomes affected the morphology and inhibited the proliferation of Caco-2 cells.

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“…While microfluidic devices can be made incredibly complex, simpler "off-the-shelf" devices have been used to achieve similar manipulations of liquid crystal systems. 26,27 A simple T-junction configuration composed of liquid chromatography components was utilized by Bottaro et al to form and load liposomes. 26 The results were compared with equivalent experiments in a chip-based microfluidic device and with the bulk approach.…”

Section: Please Do Not Adjust Marginsmentioning

confidence: 99%

“…26,27 A simple T-junction configuration composed of liquid chromatography components was utilized by Bottaro et al to form and load liposomes. 26 The results were compared with equivalent experiments in a chip-based microfluidic device and with the bulk approach. 26 Size dispersity was observed to be more consistent and lower for the "off-theshelf" device, which is particularly important for drug delivery applications.…”

Section: Please Do Not Adjust Marginsmentioning

confidence: 99%

Comparison of bulk and microfluidic methods to monitor the phase behaviour of nanoparticles during digestion of lipid-based drug formulations using in situ X-ray scattering

et al. 2019

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“Off-the-shelf” microfluidic devices for the production of liposomes for drug delivery

Cited by 28 publications

References 15 publications

Sterically stabilized liposomes production using staggered herringbone micromixer: Effect of lipid composition and PEG-lipid content

Sterically stabilized liposomes production using staggered herringbone micromixer: Effect of lipid composition and PEG-lipid content

Optimization of Conditions for Cyanidin-3-OGlucoside (C3G) Nanoliposome Production by Response Surface Methodology and Cellular Uptake Studies in Caco-2 Cells

Comparison of bulk and microfluidic methods to monitor the phase behaviour of nanoparticles during digestion of lipid-based drug formulations using in situ X-ray scattering

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