Oesophagostomum dentatum — Potential as a model for genomic studies of strongylid nematodes, with biotechnological prospects

Gasser, Robin B.; Cottee, Pauline; Nisbet, Alasdair J.; Ruttkowski, Bärbel; Ranganathan, Shoba; Joachim, Anja

doi:10.1016/j.biotechadv.2007.01.008

Cited by 19 publications

(15 citation statements)

References 120 publications

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“…Resistance of O. dentatum to levamisole, pyrantel and possibly ivermectin has been reported in Europe [21,22]. Moreover, O. dentatum also serves as a good model parasite due to its amenability to culture in the laboratory – several different life-cycle stages can be easily maintained and manipulated, and hence this worm is being increasingly used as a model parasite for biological investigations [23]. In the present study we investigated 1) whether CT have direct anthelmintic activity against O. dentatum in vitro , and 2) which stages of the life cycle of this parasite were specifically targeted by CT.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the anthelmintic activity of medicinal plant extracts and purified condensed tannins against free-living and parasitic stages of Oesophagostomum dentatum

et al. 2014

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BackgroundPlant-derived condensed tannins (CT) show promise as a complementary option to treat gastrointestinal helminth infections, thus reducing reliance on synthetic anthelmintic drugs. Most studies on the anthelmintic effects of CT have been conducted on parasites of ruminant livestock. Oesophagostomum dentatum is an economically important parasite of pigs, as well as serving as a useful laboratory model of helminth parasites due to the ability to culture it in vitro for long periods through several life-cycle stages. Here, we investigated the anthelmintic effects of CT on multiple life cycle stages of O. dentatum.MethodsExtracts and purified fractions were prepared from five plants containing CT and analysed by HPLC-MS. Anthelmintic activity was assessed at five different stages of the O. dentatum life cycle; the development of eggs to infective third-stage larvae (L3), the parasitic L3 stage, the moult from L3 to fourth-stage larvae (L4), the L4 stage and the adult stage.ResultsFree-living larvae of O. dentatum were highly susceptible to all five plant extracts. In contrast, only two of the five extracts had activity against L3, as evidenced by migration inhibition assays, whilst three of the five extracts inhibited the moulting of L3 to L4. All five extracts reduced the motility of L4, and the motility of adult worms exposed to a CT-rich extract derived from hazelnut skins was strongly inhibited, with electron microscopy demonstrating direct damage to the worm cuticle and hypodermis. Purified CT fractions retained anthelmintic activity, and depletion of CT from extracts by pre-incubation in polyvinylpolypyrrolidone removed anthelmintic effects, strongly suggesting CT as the active molecules.ConclusionsThese results suggest that CT may have promise as an alternative parasite control option for O. dentatum in pigs, particularly against adult stages. Moreover, our results demonstrate a varied susceptibility of different life-cycle stages of the same parasite to CT, which may offer an insight into the anthelmintic mechanisms of these commonly found plant compounds.

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Section: Introductionmentioning

confidence: 99%

Assessment of the anthelmintic activity of medicinal plant extracts and purified condensed tannins against free-living and parasitic stages of Oesophagostomum dentatum

et al. 2014

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“…Resistance of O. dentatum to levamisole, pyrantel and possibly ivermectin has been reported in Europe [21,22]. Moreover, O. dentatum also serves as a good model parasite due to its amenability to culture in the laboratory -several different lifecycle stages can be easily maintained and manipulated, and hence this worm is being increasingly used as a model parasite for biological investigations [23]. In the present study we investigated 1) whether CT have direct anthelmintic activity against O. dentatum in vitro, and 2) which stages of the life cycle of this parasite were specifically targeted by CT.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the anthelmintic activity of medicinal plant extracts and purified condensed tannins against free-living and parasitic stages of Oesophagostomum dentatum

Williams

Ropiak

Fryganas

et al. 2014

Parasites & Vectors

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Background: Plant-derived condensed tannins (CT) show promise as a complementary option to treat gastrointestinal helminth infections, thus reducing reliance on synthetic anthelmintic drugs. Most studies on the anthelmintic effects of CT have been conducted on parasites of ruminant livestock. Oesophagostomum dentatum is an economically important parasite of pigs, as well as serving as a useful laboratory model of helminth parasites due to the ability to culture it in vitro for long periods through several life-cycle stages. Here, we investigated the anthelmintic effects of CT on multiple life cycle stages of O. dentatum.

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“…Daugschies, 1995;Gasser et al, 2007;Joachim and Ruttkowski, 2008;Joachim et al, 1998Joachim et al, , 2001Joachim et al, , 2005Ondrovics et al, 2012Ondrovics et al, , 2013Ondrovics et al, , 2014. A number of phylogenetic studies of nuclear ribosomal DNAs have shown that O. dentatum is relatively closely related to a number of socioeconomically important nematodes of the order Strongylida, including A. duodenale, H. contortus and N. americanus (Blaxter, 1998;Chilton et al, 2006;Jex et al, 2010), rendering it a valuable model for parasitic nematodes of this order.…”

Section: Oesophagostomum Dentatum As a Model For Studying Fundamentalmentioning

confidence: 98%

Recent Advances in Elucidating Nematode Moulting – Prospects of Using Oesophagostomum dentatum as a Model

Ondrovics

Gasser

Joachim

2016

Advances in Parasitology

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Oesophagostomum dentatum — Potential as a model for genomic studies of strongylid nematodes, with biotechnological prospects

Cited by 19 publications

References 120 publications

Assessment of the anthelmintic activity of medicinal plant extracts and purified condensed tannins against free-living and parasitic stages of Oesophagostomum dentatum

Assessment of the anthelmintic activity of medicinal plant extracts and purified condensed tannins against free-living and parasitic stages of Oesophagostomum dentatum

Assessment of the anthelmintic activity of medicinal plant extracts and purified condensed tannins against free-living and parasitic stages of Oesophagostomum dentatum

Recent Advances in Elucidating Nematode Moulting – Prospects of Using Oesophagostomum dentatum as a Model

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