Oenothera laciniata inhibits lipopolysaccharide induced production of nitric oxide, prostaglandin E2, and proinflammatory cytokines in RAW264.7 macrophages

Yoon, Weon‐Jong; Ham, Young-Min; Yoo, Byoung-Sam; Moon, Ji‐Young; Koh, Jung Hee; Hyun, Chang‐Gu

doi:10.1016/j.jbiosc.2008.11.018

Cited by 55 publications

(31 citation statements)

References 27 publications

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“…The patches (chambers) remained in place for 48 h. During this time, the subjects abstained from showering or performing any work or exercise that might wet or loosen the patches. Once the patches were removed, the site was evaluated at 30 min and 24 h after application; the readings were scored according to the Cosmetic, Toiletry, and Fragrance Association (CTFA) guidelines [18].…”

Section: Human Skin Primary Irritation Testmentioning

confidence: 99%

Anti-inflammatory effect of chitosan oligosaccharides in RAW 264.7 cells

Yang¹,

Kim²,

Kim³

et al. 2010

Open Life Sciences

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Anti-inflammatory effect of chitosan oligosaccharides in RAW 264.7 cells * E-mail: cghyun@jejuhidi.or.kr Abstract: We examined the effects of chitosan oligosaccharides (COSs) with different molecular weights (COS-A, 10 kDa < MW < 20 kDa; COS-C, 1 kDa < MW < 3 kDa) on the lipopolysaccharide (LPS)-induced production of prostaglandin E 2 and nitric oxide and on the expression of cyclooxygenase-2 and inducible nitric oxide synthase in RAW264.7 macrophages. COS-A (0.4%) and COS-C (0.2%) significantly inhibited PGE 2 production in LPS-stimulated macrophages without cytotoxicity. The effect of COS-A and COS-C on COX-2 expression in activated macrophages was also investigated by immunoblotting. The inhibition of PGE 2 by COS-A and COS-C can be attributed to the blocking of COX-2 protein expression. COS-A (0.4%) and COS-C (0.2%) also markedly inhibited the LPS-induced NO production of RAW 264.7 cells by 50.2% and 44.1%, respectively. The inhibition of NO by COSs was consistent with decreases in inducible nitric oxide synthase (iNOS) protein expression. To test the inhibitory effects of COS-A and COS-C on other cytokines, we also performed ELISA assays for IL-1ß in LPS-stimulated RAW 264.7 macrophage cells, but only a dose-dependent decrease in the IL-1ß production exerted by COS-A was observed. In order to test for irritation and the potential sensitization of COS-A and COS-C for use as cosmetic materials, human skin primary irritation tests were performed on 32 volunteers; no adverse reactions of COSs usage were observed. Based on these results, we suggest that COS-A and COS-C be considered possible anti-inflammatory candidates for topical application.

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Section: Human Skin Primary Irritation Testmentioning

confidence: 99%

Anti-inflammatory effect of chitosan oligosaccharides in RAW 264.7 cells

Yang¹,

Kim²,

Kim³

et al. 2010

Open Life Sciences

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“…The control of macrophage overproduction of inflammatory mediators such as prostaglandin E 2 (PGE 2 ) and NO should greatly facilitate the treatment of inflammatory diseases. PGE 2 synthesis is catalyzed by cyclooxygenase-2 (COX-2), which is induced in macrophages by the same stimulus as iNOS [6,7]. The expression of iNOS and COX-2 is closely related to the up-regulation of nuclear factor-kappaB (NF-κB).…”

Section: Introductionmentioning

confidence: 99%

Bisabolangelone isolated from Ostericum koreanum inhibits the production of inflammatory mediators by down-regulation of NF-κB and ERK MAP kinase activity in LPS-stimulated RAW264.7 cells

Jung

Mahesh

Park³

et al. 2010

International Immunopharmacology

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“…HPLC fingerprint, a chromatographic method, was highly recommended for developing standard substances from raw materials 17 . We used HPLC fingerprint technology to determine the presence of coumestrol, a component of rape plants known to have anti-inflammatory properties.…”

Section: Resultsmentioning

confidence: 99%

Anti-Inflammatory Potential of Rape Flower in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

Hyun¹,

Kang²,

Yang³

et al. 2016

Orient. J. Chem

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The present study was undertaken to investigate the anti-inflammatory potential and mechanism of action of the rape flower extract (RFE) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. RFE inhibited nitric oxide (NO), prostaglandin E 2 (PGE 2), interleukin-1â (IL-1â), and IL-6 production in a concentration-dependent manner. RFE effectively attenuated the expression of inflammation-mediating enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), at the protein level in a concentration-dependent manner. Additionally, the attenuation of inflammatory responses in RAW 264.7 cells by RFE was closely associated with the suppression of the phosphorylation of extracellular signal-regulated kinase (ERK), eventually blocking the activation of downstream elements contributing to inflammation. RFE HPLC fingerprint indicated the presence of coumestrol. The coumestrol content in RFE was 2.614 ìM. To evaluate the safety of RFE and its effects on the human skin, human skin primary irritation tests were performed on the normal skin (upper back) of 32 volunteers to determine if any constituent of RFE presented an irritation or sensitization potential. RFE did not induce any adverse reactions. Taken together, our results suggest that RFE may be considered as an anti-inflammatory candidate for topical application.

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Oenothera laciniata inhibits lipopolysaccharide induced production of nitric oxide, prostaglandin E2, and proinflammatory cytokines in RAW264.7 macrophages

Cited by 55 publications

References 27 publications

Anti-inflammatory effect of chitosan oligosaccharides in RAW 264.7 cells

Anti-inflammatory effect of chitosan oligosaccharides in RAW 264.7 cells

Bisabolangelone isolated from Ostericum koreanum inhibits the production of inflammatory mediators by down-regulation of NF-κB and ERK MAP kinase activity in LPS-stimulated RAW264.7 cells

Anti-Inflammatory Potential of Rape Flower in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

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