2013
DOI: 10.1101/pdb.prot071639
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Odor–Taste Learning Assays in Drosophila Larvae

Abstract: The Drosophila larva is an emerging model for studies in behavioral neurogenetics because of its simplicity in terms of cell number. Despite this simplicity, basic features of neuronal organization and key behavior faculties are shared with adult flies and with mammals. Here, we describe a Pavlovian-type learning assay in fruit fly larvae. A group of larvae is sequentially exposed to specific odors in the presence or the absence of sugar, and then tested to determine whether they prefer the odor previously exp… Show more

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Cited by 36 publications
(38 citation statements)
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“…After three such training cycles, larvae are tested for their choice between AM versus OCT (for half of the cases, the sequence of training trials is reversed: OCT/AM+ and AM/OCT+). From the difference in preference between the reciprocally trained groups the performance index is calculated to quantify associative learning (for details, see Gerber et al, 2010). (B)Associative function is reduced by ~50% in syn 97 mutant larvae relative to CS wild-type larvae; this effect is also seen in the w 1118 background.…”
Section: Behavioural Paradigms For Associative Learning In Larval Dromentioning
confidence: 99%
“…After three such training cycles, larvae are tested for their choice between AM versus OCT (for half of the cases, the sequence of training trials is reversed: OCT/AM+ and AM/OCT+). From the difference in preference between the reciprocally trained groups the performance index is calculated to quantify associative learning (for details, see Gerber et al, 2010). (B)Associative function is reduced by ~50% in syn 97 mutant larvae relative to CS wild-type larvae; this effect is also seen in the w 1118 background.…”
Section: Behavioural Paradigms For Associative Learning In Larval Dromentioning
confidence: 99%
“…To evaluate the consequences of the egg-laying decisions of females on the cognitive abilities of larvae, we measured the learning performances of third instar larvae reared on three different breeding diets using a well-established reciprocal, differential conditioning assay for olfactory learning (Gerber et al, 2013). Fifteen groups of five females reared on a standard diet were transferred to culture tubes (55 ml) containing a high carbohydrate diet (P:C 1:16, P+C 180 g l −1 ), a high protein diet (P:C 8:1, P+C 180 g l −1…”
Section: Experiments 7: Effect Of Breeding Diets On Larval Cognitionmentioning
confidence: 99%
“…Learning experiments follow standard methods (Scherer et al 2003;Neuser et al 2005; for a detailed protocol see Gerber et al 2010) (sketch in Fig. 1C), employing a two-odor, reciprocal conditioning paradigm, unless mentioned otherwise.…”
Section: Associative Learning Experimentsmentioning
confidence: 99%