Odontoblast‐like cell numbers and reparative dentine thickness after direct pulp capping with platelet‐rich plasma and enamel matrix derivative: a histomorphometric evaluation

Orhan, Ekim Onur; Maden, Murat; Senguuven, B.

doi:10.1111/j.1365-2591.2011.01977.x

Cited by 45 publications

(39 citation statements)

References 42 publications

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“…It should be noted that different animals, and different types of teeth of the same animal, might generate different results. For example, in a study using rat incisors to test direct pulp-capping via mineral trioxide aggregate (MTA), desirable regenerative ability was obtained with TDT of 200 μm [51]. These TDT values were greater than the TDT of the present study using molar teeth, which might be due to the superior regenerative capacity of the continuously-growing rat incisors.…”

Section: Discussioncontrasting

confidence: 53%

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Evaluation of antibacterial and remineralizing nanocomposite and adhesive in rat tooth cavity model

Wang

Weir

et al. 2014

Acta Biomaterialia

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Antibacterial and remineralizing dental composites and adhesives were recently developed to inhibit biofilm acids and combat secondary caries. It is not clear what effect these materials will have on dental pulps in vivo. The objectives of this study were to investigate the antibacterial and remineralizing restorations in a rat tooth cavity model, and determine pulpal inflammatory response and tertiary dentin formation. Nanoparticles of amorphous calcium phosphate (NACP) and antibacterial dimethylaminododecyl methacrylate (DMADDM) were synthesized and incorporated into a composite and an adhesive. Occlusal cavities were prepared in the first molars of rats and restored with four types of restoration: Control composite and adhesive; control plus DMADDM; control plus NACP; and control plus both DMADDM and NACP. At 8 or 30 days (d), rat molars were harvested for histological analysis. For inflammatory cell response, regardless of time periods, NACP group and DMADDM+NACP group showed lower scores (better biocompatibility) than control group (p = 0.014 for 8 d, p = 0.018 for 30 d). For tissue disorganization, NACP and DMADDM+NACP had better scores than control (p = 0.027) at 30 d. At 8 d, restorations containing NACP had tertiary dentin thickness (TDT) that was 5-6 fold that of control. At 30 d, restorations containing NACP had TDT that was 4-6 fold that of control. In conclusion, novel antibacterial and remineralizing restorations were tested in rat teeth in vivo for the first time. Composite and adhesive containing NACP and DMADDM exhibited milder pulpal inflammation and much greater tertiary dentin formation, than control adhesive and composite. Therefore, the novel composite and adhesive containing NACP and DMADDM are promising as a new therapeutic restorative system to not only combat oral pathogens and biofilm acids as shown previously, but also facilitate the healing of the dentin-pulp complex.

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Section: Discussioncontrasting

confidence: 53%

“…The use of two teeth per rat followed previous studies [33,34]. The sample size was the number of tooth cavities, not the number of animals, following previous studies [51,52]. The sample size of n = 6 was also consistent with previous studies [47,52].…”

Section: Methodsmentioning

confidence: 85%

Evaluation of antibacterial and remineralizing nanocomposite and adhesive in rat tooth cavity model

Wang

Weir

et al. 2014

Acta Biomaterialia

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“…In the last decade, many experimental and clinical studies have been carried out to develop and test new dental materials endowed with safe biocompatibility and antiinfective properties (26)(27)(28). The material used should provide an appropriate host response.…”

Section: Discussionmentioning

confidence: 99%

In vitro cytotoxicity evaluation of different pulp capping materials: a comparative study

Poggio

Ceci

Dagna

et al. 2015

Archives of Industrial Hygiene and Toxicology

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Direct pulp capping covers the exposed surface of the pulp to maintain its vitality and preserve its functional and biologic activity. The aim of the present study was to compare the biocompatibility effects of seven different pulp-capping materials in vitro: Dycal®, Calcicur®, Calcimol LC®, TheraCal LC®, ProRoot MTA®, MTA-Angelus®, and Biodentine®. Using the Transwell insert methodology by Alamar blue test, we evaluated the cytocompatibility of the above mentioned materials towards murine odontoblasts cells (MDPC-23) at three different times (24, 48, and 72 h). For additional control, the cell viability at 72 hours was also assessed by MTT assay. Morphological analysis of murine odontoblasts was assessed by Confocal Laser Scanning Microscope. The results indicate significantly different biocompatibility among materials with different composition. Biodentine® and mineral trioxide aggregate (MTA)-based products showed lower cytotoxicity, varying from calcium hydroxide-based materials, which exhibited higher cytotoxicity. Although our findings are limited to in vitro conditions, the observation that Biodentine® caused a cytotoxic effect similar to MTA suggests that it may be considered an alternative in pulp-capping treatment, as calcium hydroxide-based materials present higher cytotoxic effects.

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“…11,12 Biological modulators identified during teeth development, for example, recombinant human bone morphogenetic protein 13–15 or enamel matrix derivative (EMD; Emdogain ® gel) 16 or combination of both, 17 have been proposed as pulp capping materials that would enhance dental tissues’ regeneration.…”

Section: Introductionmentioning

confidence: 99%

Root maturation and dentin–pulp response to enamel matrix derivative in pulpotomized permanent teeth

et al. 2014

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The success of pulpotomy of young permanent teeth depends on the proper selection of dressing materials. This study aimed to evaluate the histological and histomorphometric response of dentin–pulp complex to the enamel matrix derivative (Emdogain® gel) compared to that of calcium hydroxide when used as a pulp dressing in immature young permanent dogs’ teeth. Dentin-like tissues bridging the full width of the coronal pulp at the interface between the injured and healthy pulp tissues were seen after 1 month in both groups. With time, the dentin bridge increased in thickness for calcium hydroxide but disintegrated and fully disappeared for Emdogain-treated group. Progressive inflammation and total pulp degeneration were only evident with Emdogain-treated group. The root apices of Emdogain-treated teeth became matured and closed by cementum that attached to new alveolar bone by a well-oriented periodontal ligament. In young permanent dentition, Emdogain could be a good candidate for periodontium but not dentino–pulpal complex regeneration.

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Odontoblast‐like cell numbers and reparative dentine thickness after direct pulp capping with platelet‐rich plasma and enamel matrix derivative: a histomorphometric evaluation

Cited by 45 publications

References 42 publications

Evaluation of antibacterial and remineralizing nanocomposite and adhesive in rat tooth cavity model

Evaluation of antibacterial and remineralizing nanocomposite and adhesive in rat tooth cavity model

In vitro cytotoxicity evaluation of different pulp capping materials: a comparative study

Root maturation and dentin–pulp response to enamel matrix derivative in pulpotomized permanent teeth

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