Ocular toxoplasmosis after the fifth decade

Labalette, P.; Delhaès, Laurence; Margaron, Fabrice; Fortier, B; Jf, Rouland

doi:10.1016/s0002-9394(02)01324-7

Cited by 106 publications

(62 citation statements)

References 42 publications

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“…Although this disease has long been considered as the reactivation of a congenital infection (33), there is now clear evidence that acquired toxoplasmosis can also induce ocular lesions (11,30,40).…”

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confidence: 99%

“…Laboratory confirmation of ocular toxoplasmosis may be asserted in 50 to 80% of patients by analyzing paired samples of aqueous or vitreous humor and serum for the detection of local specific antibodies (23,26,45) or by using conventional gene amplification techniques (4,30,45). More recently, realtime PCR has been developed to improve Toxoplasma infection diagnosis (6,9,10,29,31).…”

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confidence: 99%

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Use of Fluorescence Resonance Energy Transfer Hybridization Probes To Evaluate Quantitative Real-Time PCR for Diagnosis of Ocular Toxoplasmosis

Simon¹,

Labalette²,

Ordinaire³

et al. 2004

J Clin Microbiol

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Toxoplasma gondii infection is an important cause of chorioretinitis in Europe and the United States.Ophthalmological examination and a good clinical response to adequate therapy mainly support ocular toxoplasmosis diagnosis. However, clinical diagnostic may be difficult in some atypical cases. In these cases, laboratory confirmation, based on detection of local specific antibodies and parasite DNA by conventional PCR, is therefore important to confirm the disease etiology. More recently, real-time PCR has been developed to improve prenatal congenital toxoplasmosis diagnosis. We therefore examined the diagnostic value of quantitative real-time PCR for the detection of T. gondii in aqueous humor samples, associated with quantification of human ␤-globin to control sample quantitative quality, by using a double fluorescence resonance energy transfer hybridization probes system with a double fluorescence reading. Of the 23 the clinically toxoplasmosis suspect patients, 22 showed serological evidence of exposure to Toxoplasma; one had a serological profile indicative of active infection. The analysis of paired aqueous humor and serum samples revealed an intraocular antibody production in 9 of 23 cases (39.1%). The quantitative real-time PCR revealed positive and high parasite numbers and high Toxoplasma/human genome ratios in three cases. Furthermore, PCR was the only positive confirmatory test in two cases (11.1%). None of the patients included in the control group (n ‫؍‬ 7) had evidence of either local specific antibody production or T. gondii DNA detection, suggesting a good relative assay specificity. On the whole, quantitative real-time PCR appears to be useful for diagnosing atypical ocular toxoplasmosis presentations.

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confidence: 99%

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confidence: 99%

Use of Fluorescence Resonance Energy Transfer Hybridization Probes To Evaluate Quantitative Real-Time PCR for Diagnosis of Ocular Toxoplasmosis

Simon¹,

Labalette²,

Ordinaire³

et al. 2004

J Clin Microbiol

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“…Similar lesions may also be encountered during the use of immunosuppressive drugs [9,14]. Many studies have focused on elderly patients [15][16][17]. These patients can have large and multiple ocular lesions with severe vitritis and prolonged disease, in some instances similar to lesions encountered in immunocompromissed individuals, although they are otherwise healthy.…”

Section: Occurrence Depends On Host Genetic Background and Immune Statusmentioning

confidence: 95%

“…OT is also confirmed by a favorable clinical response to specific therapy. However, diagnosis and treatment can be delayed in patients with atypical lesions (unusual and complicated forms) or patients showing an inadequate response to antimicrobial therapy as particularly observed in elderly or immunocompromised patients [13,17]. In such cases, rapid identification of the causative agent requires aqueous humor sampling by anterior chamber paracentesis [59].…”

Section: Diagnosis Is Based On Clinical Signs and Some Selected Biolomentioning

confidence: 99%

Risk Factors, Pathogenesis and Diagnosis of Ocular Toxoplasmosis

Dupouy‐Camet¹,

Talabani²,

Delair³

et al. 2012

Toxoplasmosis - Recent Advances

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“…This fact often leads to difficult diagnosis, leading to confusion with other causes of chorioretinitis and consequently to a delay in treatment or the beginning of an inadequate treatment since it can be misdiagnosed as other pathologies such as herpetic ones [3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%