Octulose phosphates from the human red blood cell

Bartlett, Grant R.; Bucolo, Giovanni

doi:10.1016/0006-291x(60)90158-3

Cited by 21 publications

(4 citation statements)

References 6 publications

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“…The resulting SBP is then cleaved by aldolase to generate E4P and DHAP (Nakahigashi et al, 2009). Sedoheptulose and octulose compounds have also been observed in human tissues (Bartlett and Bucolo, 1960; Bucolo and Bartlett, 1960). In macrophages stimulated by endotoxin, a sedoheptulose kinase that produces S7P from sedoheptulose must be downregulated for proper activation (A. Haschemi, personal communication).…”

Section: Discussionmentioning

confidence: 99%

Riboneogenesis in Yeast

Clasquin

Melamud

Singer

et al. 2011

Cell

111

129

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Summary Gluconeogenesis converts three carbon units into glucose. Here we identify an analogous pathway in Saccharomyces cerevisiae for converting three carbon units into ribose, a component of nucleic acids and nucleotides. This riboneogenic pathway involves the enzyme sedoheptulose-1,7-bisphosphatase (SHB17), whose activity was identified based on accumulation of sedoheptulose-1,7-bisphosphate in the corresponding knockout strain. We determined the crystal structure of Shb17 in complex with sedoheptulose-1,7-bisphosphate, and found that the sugar is bound in the closed furan form in the active site. Like fructose-1,6-bisphosphate, sedoheptulose-1,7-bisphosphate is produced by aldolase, in this case from erythrose 4-phosphate and dihydroxyacetone phosphate. Hydrolysis of sedoheptulose-1,7-bisphosphate by SHB17 provides an energetically favorable input to the non-oxidative pentose phosphate pathway to drive ribose production. Flux through SHB17 is enhanced under conditions when ribose demand is high relative to demand for NADPH, including during ribosome biogenesis in metabolically synchronized yeast cells. Thus, riboneogenesis provides a thermodynamically-driven route of ribose production uncoupled from formation of NADPH.

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Section: Discussionmentioning

confidence: 99%

Riboneogenesis in Yeast

Clasquin

Melamud

Singer

et al. 2011

Cell

111

129

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“…Streptomyces lincolnensis ; Sasaki et al , 2012 ), fungi (e.g. Saccharomyces cerevisiae ; Clasquin et al , 2011 ), a protozoan parasite ( Trypanosoma brucei ; Creek et al , 2012 ), animals (rat liver; Paoletti et al , 1979 ), human red blood cells ( Bartlett and Bucolo, 1960 ) and human erythrocytes ( Vanderheiden, 1965 ). Therefore, it is reasonable to assume that octulose is in fact much more widespread among living organisms.…”

Section: Physiological Functionsmentioning

confidence: 99%

“…The eight-carbon monosaccharide octulose was found in plants, animals and humans many years ago ( Bartlett and Bucolo, 1960 ; Charlson and Richtmyer, 1960 ; Bartlett, 1970 ). As an eight-carbon ketose, it can exist in isomeric forms; except for the 3-octulose D- gluco -L- glycero -3-octulose found in young leaves of Laurus nobilis ( Sakata et al , 1989 ), the other reported octulose isomers in plants are all 2-octuloses and include D- glycero -D- manno -octulose, D- glycero -L- galacto -octulose, L- glycero -L- galacto -octulose, D- glycero -D- altro -octulose and D- glycero -D- ido -octulose ( Table 1 ; see structures in Fig.…”

mentioning

confidence: 99%

Octulose: a forgotten metabolite?

Zhang

Bartels²

2017

Journal of Experimental Botany

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“…Isomer of octulose phosphate Molarity of ammonium carbonate required for elution from formate column (Bartlett & Bucolo, 1968) RP04 in solvent C 2max. (nm) with cysteine/H2SO4 reagent A410/A485 (Bartlett & Bucolo, 1960 xylulose, arabinose and ribose were identified, thus it was concluded that these pentoses were derived from xylulose 5-phosphate, arabinose 5-phosphate and ribose 5-phosphate respectively. The concentration of arabinose 5-phosphate increased to a maximum at 0.5h and then slowly decreased during the remaining period of the incubation (Table 3).…”

Section: Kinetics Of Transaldolase Inhibition By Arabinose 5-phosphatementioning

confidence: 99%

New reaction sequences for the non-oxidative pentose phosphate pathway

Williams¹,

Blackmore²,

Clark³

1978

Biochemical Journal

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1. Reactions leading to the formation of 14C-labelled volatile compounds and compounds volatile under acid conditions were investigated in a system actively synthesizing hexose 6-phosphates from [U-14C]ribose 5-phosphate by reactions catalysed by enzymes prepared from acetone-dried powder of rat liver; no reactions involving 14C-labelled volatile compounds were detected. Similarly the fixation of 14C-labelled volatile compounds into hexose 6-phosphate could not be detected. 2. A complete carbon balance was made for the reactants, intermediates and products of the reactions involved in the conversion of ribose 5-phosphate into hexose 6-phosphate by enzymes of rat liver. Five additional intermediates of pentose 5-phosphate metabolism in liver were detected, namely D-manno-heptulose 7-phosphate, D-altro-heptulose 1,7-bisphosphate, D-glycero-D-ido-octulose 1,8-bisphosphate, D-glycero-D-altro-octulose 1,8-bisphosphate and D-arabinose 5-phosphate. 3. D-Arabinose 5-phosphate was found to be utilized by a rat liver enzyme preparation to produce both hexose 6-phosphate and triose phosphate. 4. D-Arabinose 5-phosphate was reversibly converted into other pentose 5-phosphates. Paper chromatographic and enzymic evidence indicated that the conversion involved an enzyme tentatively named arabinose phosphate 2-epimerase, which catalyses the following reaction: D-arabinose 5-P in equilibrium D-ribose-5-P. 5. A variety of rat tissues also utilized D-arabinose 5-phosphate to produce both hexose 6-phosphate and triose phosphate and at a rate comparable with that obtained with D-ribose 5-phosphate. 6. A new reaction sequence for the non-oxidative pentose phosphate pathway in liver is proposed.

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Octulose phosphates from the human red blood cell

Cited by 21 publications

References 6 publications

Riboneogenesis in Yeast

Riboneogenesis in Yeast

Octulose: a forgotten metabolite?

New reaction sequences for the non-oxidative pentose phosphate pathway

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