Octulose: a forgotten metabolite?

Zhang, Qingwei; Bartels, Dorothea

doi:10.1093/jxb/erx367

Cited by 15 publications

(22 citation statements)

References 54 publications

(65 reference statements)

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“…Interestingly, the authors proposed a new connection between glycolysis and the PPP, in that sedoheptulose-1,7-bisphosphate is formed by the aldolase, condensing dihydroxyacetone phosphate and erythrose-4-phosphate in a reversible reaction ( Figure 1 ; [ 25 ]). Similarly, they propose that the octulose bisphosphates could be formed by condensation of DHAP with ribose-5-phosphate, an important reaction that also occurs in plants [ 72 ]. Sedoheptulose-7-phosphate has also been reported to be converted to the bisphosphate by a side reaction of pyrophosphate-dependent phosphofructokinases (PFK) from bacteria [ 73 , 74 ].…”

Section: Overview On the Reactions Of The Pentose Phosphate Pathway (Ppp)mentioning

confidence: 99%

The Pentose Phosphate Pathway in Yeasts–More Than a Poor Cousin of Glycolysis

2021

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The pentose phosphate pathway (PPP) is a route that can work in parallel to glycolysis in glucose degradation in most living cells. It has a unidirectional oxidative part with glucose-6-phosphate dehydrogenase as a key enzyme generating NADPH, and a non-oxidative part involving the reversible transketolase and transaldolase reactions, which interchange PPP metabolites with glycolysis. While the oxidative branch is vital to cope with oxidative stress, the non-oxidative branch provides precursors for the synthesis of nucleic, fatty and aromatic amino acids. For glucose catabolism in the baker’s yeast Saccharomyces cerevisiae, where its components were first discovered and extensively studied, the PPP plays only a minor role. In contrast, PPP and glycolysis contribute almost equally to glucose degradation in other yeasts. We here summarize the data available for the PPP enzymes focusing on S. cerevisiae and Kluyveromyces lactis, and describe the phenotypes of gene deletions and the benefits of their overproduction and modification. Reference to other yeasts and to the importance of the PPP in their biotechnological and medical applications is briefly being included. We propose future studies on the PPP in K. lactis to be of special interest for basic science and as a host for the expression of human disease genes.

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Section: Overview On the Reactions Of The Pentose Phosphate Pathway (Ppp)mentioning

confidence: 99%

The Pentose Phosphate Pathway in Yeasts–More Than a Poor Cousin of Glycolysis

2021

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“…38 Additional heptulose, octulose, and nonulose molecules have been previously described. 39 These molecules are most commonly found in plants but also appear in bacteria and mammals. 39 The concentrations of these monosaccharides vary significantly.…”

Section: ■ Discussionmentioning

confidence: 99%

Functional Characterization of YdjH, a Sugar Kinase of Unknown Specificity in Escherichia coli K12

Huddleston

Raushel

2019

Biochemistry

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The ydj gene cluster is annotated to catalyze the catabolism of an unknown carbohydrate. Previously, YdjI, a class II aldolase, was shown to catalyze the retro-aldol cleavage of l-glycero-l-galacto-octuluronate-1-phosphate into DHAP and l-arabinuronate. In this report, the functional characterization of YdjH is presented. YdjH catalyzes the phosphorylation of 2-keto-monosaccharides at the C1 hydroxyl group with a substrate profile significantly more stringent than that of YdjI. Similar to YdjI, YdjH shows a strong preference for higher-order monosaccharides (seven to nine carbons) with a carboxylate terminus. The best substrate was determined to be l-glycero-l-galacto-octuluronate, yielding l-glycero-l-galacto-octuluronate-1-phosphate with a k cat of 16 s–1 and a k cat/K m of 2.1 × 104 M–1 s–1. This is apparently the first reported example of kinase activity with eight-carbon monosaccharides. Two crystal structures of YdjH were previously determined to 2.15 and 1.8 Å resolution (Protein Data Bank entries 3H49 and 3IN1). We present an analysis of the active site layout and use computational docking to identify potential key residues in the binding of l-glycero-l-galacto-octuluronate.

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“…The desiccation-tolerant C. plantagineum possesses high amounts of the unusual monosaccharide octulose, which is found in a few other plants as well [ 190 , 191 ]. The desiccation-tolerant L. brevidens has more octulose than the sensitive L. subracemosa and a positive correlation between octulose and desiccation tolerance was shown within the Linderniaceae [ 191 ]. Octulose is hypothesized to play a role in carbohydrate storage and ROS scavenging [ 191 ].…”

Section: Metabolome Reconfigurations In Resurrection Plants During Desiccationmentioning

confidence: 99%

“…The desiccation-tolerant L. brevidens has more octulose than the sensitive L. subracemosa and a positive correlation between octulose and desiccation tolerance was shown within the Linderniaceae [ 191 ]. Octulose is hypothesized to play a role in carbohydrate storage and ROS scavenging [ 191 ]. During desiccation, when photosynthesis is impaired, carbohydrate reserves such as starch and octulose become especially important not only as an energy source but also for the synthesis of protective sugars (sucrose, raffinose, stachyose), antioxidants, secondary metabolites (anthocyanins, etc.…”

Section: Metabolome Reconfigurations In Resurrection Plants During Desiccationmentioning

confidence: 99%

Systems biology of resurrection plants

Gechev

Lyall

Petrov

et al. 2021

Cell. Mol. Life Sci.

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Plant species that exhibit vegetative desiccation tolerance can survive extreme desiccation for months and resume normal physiological activities upon re-watering. Here we survey the recent knowledge gathered from the sequenced genomes of angiosperm and non-angiosperm desiccation-tolerant plants (resurrection plants) and highlight some distinct genes and gene families that are central to the desiccation response. Furthermore, we review the vast amount of data accumulated from analyses of transcriptomes and metabolomes of resurrection species exposed to desiccation and subsequent rehydration, which allows us to build a systems biology view on the molecular and genetic mechanisms of desiccation tolerance in plants.

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Octulose: a forgotten metabolite?

Cited by 15 publications

References 54 publications

The Pentose Phosphate Pathway in Yeasts–More Than a Poor Cousin of Glycolysis

The Pentose Phosphate Pathway in Yeasts–More Than a Poor Cousin of Glycolysis

Functional Characterization of YdjH, a Sugar Kinase of Unknown Specificity in Escherichia coli K12

Systems biology of resurrection plants

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