Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin

Seiser, Saskia; Janker, Lukas; Zila, Nina; Mildner, Michael; Rakita, Ana; Matiasek, Johannes; Bileck, Andrea; Gerner, Christopher; Paulitschke, Verena; Elbe‐Bürger, Adelheid

doi:10.1038/s41598-020-79378-9

Cited by 24 publications

(27 citation statements)

References 45 publications

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“…28 In addition, octenidine contributes to the healing of chronic wounds via the inhibition of the production and activity of matrix metalloproteinase and other eukaryotic proteases. 29,30 Several bacterial species produce proteases as virulence factors that contribute to the degradation of wound granulation tissue, and octenidine has even been observed to inhibit proteases secreted by bacteria that were isolated from chronic wounds. 30 In summary, octenidine positively affects the healing of chronic human wounds.…”

Section: Introductionmentioning

confidence: 99%

Silver distribution in chronic wounds and the healing dynamics of chronic wounds treated with dressings containing silver and octenidine

et al. 2021

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Although silver is an efficient antimicrobial and is a widely used antiseptic in wound healing, previous studies have reported the cytotoxic in vitro effects of silver dressings. Moreover, few studies have addressed the distribution of silver in chronic wounds. The study compares the healing of chronic wounds treated with a standardof-care silver dressing (Ag-CMC) and a dressing containing antiseptic octenidine (OCT-HA). Biopsies were taken from two wound areas before the commencement of treatment (baseline), after 2 weeks and after 6 weeks (the end of the study). We analyzed the histopathologic wound-healing score, silver distribution, and expression of selected genes. The wound-healing score improved significantly in the wounded area treated with OCT-HA after 2 weeks compared to the baseline and the Ag-CMC. The Ag-CMC wound areas improved after 6 weeks compared to the baseline. Moreover, collagen maturation and decreases in the granulocyte and macrophage counts were faster in the OCT-HA parts. Treatment with OCT-HA resulted in less wound slough.The silver, visualized via autometallography, penetrated approximately 2 mm into the wound tissue and associated around capillaries and ECM fibers, and was detected in phagocytes. The metallothionein gene expression was elevated in the Ag-CMC wound parts. This exploratory study determined the penetration of silver into human chronic wounds and changes in the distribution thereof during treatment. We observed that silver directly affects the cells in the wound and elevates the metallothionein gene expression. Octenidine and hyaluronan dressings provide a suitable alternative to silver and carboxymethyl cellulose dressings without supplying silver to the wound.

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Section: Introductionmentioning

confidence: 99%

Silver distribution in chronic wounds and the healing dynamics of chronic wounds treated with dressings containing silver and octenidine

et al. 2021

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“…Незважаючи на високу антимікробну ефективність, октенідину дигідрохлорид не чинить негативного впливу на епітеліальну тканину ШМ і піхви. За хімічною природою октенідин є поверхнево активною речовиною, що зв'язує і відокремлює ліпіди від оболонок інфектантів за принципами електростатики [32,35]. Основною мішенню для октенідину, як і для інших катіонних антисептиків, є гліцерофосфат мембран бактеріальних клітин.…”

Section: вступunclassified

“…Основною мішенню для октенідину, як і для інших катіонних антисептиків, є гліцерофосфат мембран бактеріальних клітин. Активні позитивно заряджені хвости молекул октенідину з'єднуються з негативно зарядженими ліпідами мембрани патогенів, взаємодіють із полісахаридами в клітинній стінці мікроорганізмів і цитоплазматичними бактеріальними ферментними системами, змінюють поверхневий натяг на межі розподілу середовищ і виривають із неї ліпіди, порушуючи таким чином цілісність клітин, що призводить до руйнування цитоплазматичної мембрани і загибелі бактеріальної клітини [32].…”

Section: вступunclassified

“…Завдяки цьому відбувається подальше тривале зниження числа мікробів. Крім катіонної молекулярної будови, спостережувана реманентність обумовлена зокрема тим фактом, що октенідин не поглинається вагінальною слизовою оболонкою і таким чином довше зберігається на поверхні [32].…”

Section: вступunclassified

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Effectiveness of local application of antiseptic with octenidin dihydrochloride molecule in smoking women with ASC-US and LSIL

Akulina

2021

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This article presents the results of our own experience of topical use of the Prodexin antiseptic that is contained an octenidine dihydrochloride molecule as a monotherapy for ASC-US and LSIL cervical pathology (identified with in fluid cytology) associated with human papillomavirus in smoking women.Study objective: To study the efficacy of topical application of the octenidine dihydrochloride molecule in the Prodexin vaginal suppositories in the treatment of smoking women with ASC-US and LSIL.Materials and methods. The study involved 45 female smokers with identified ASC-US or LSIL. The complex of examination included: Pap test based on liquid cytology, testing for human papillomavirus 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68 types in a quantitative format, screening for the causes of vaginitis, extended colposcopy to exclude cervical intraepithelial neoplasia II+. Prodexin suppositories were used according to a 3-month treatment regimen (20 suppositories daily, and then 10 suppositories in two consecutive cycles).Results. Clinical and laboratory assessment of the therapy effectiveness has convincingly shown its positive effect on the vagina and cervix. Treatment with the Prodexin antiseptic associated with microbiota normalization, colposcopic picture improvement and changes in the Pap smear to NILM I or II. Regress to NILM was recorded in 20 (86.9%) patients with ASC-US and in 15 (68.8%) women with LSIL.Conclusions. Prodexin allows to avoid a worsening of the situation and prevent the changes progression in HSIL. Proposed treatment had no side effects, was convenient to use and did not require additional visits to doctor during therapy. The control over the human papillomavirus and study of the viral load has been postponed for 6–12 months from the beginning of the examination, that will be the second stage of work.

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“…The selection of topical active agents is significant for the preparation of new multifunctional chronic wound healing materials. Octenidine dihydrochloride (OCT) is a widely used antiseptic that promotes skin wound healing and improves scar quality after burns and surgical interventions [24]. It is an alkanediylbis[pyridine] germicidal agent with structural similarity to chlorhexidine and benzalkonium chloride [25,26].…”

Section: Introductionmentioning

confidence: 99%

3D Printing of Thermoresponsive Hydrogel Laden with an Antimicrobial Agent towards Wound Healing Applications

et al. 2021

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Thermoresponsive hydrogel-based wound dressings with an incorporated antimicrobial agent can be fabricated employing 3D printing technology. A novel printable ink containing poly(N-isopropylacrylamide) (PNIPAAm) precursors, sodium alginate (ALG), methylcellulose (MC) that is laden with a mixture of octenidine dihydrochloride and 2-phenoxyethanol (Octenisept®, OCT) possess accurate printability and shape fidelity. This study also provides the protocol of ink’s use for the 3D printing of hydrogel scaffolds. The hydrogel’s physicochemical properties and drug release profiles from the hydrogel specimens to the external solution have been determined at two temperatures (20 and 37 °C). The release test showed a sustained OCT delivery into ultrapure water and the PBS solution. The temperature-responsive hydrogel exhibited antimicrobial activity against Staphylococcus aureus, Candida albicans, and Pseudomonas aeruginosa and demonstrated non-cytotoxicity towards fibroblasts. The thermoresponsive behavior along with biocompatibility, antimicrobial activity, and controlled drug release make this hydrogel a promising class of materials for wound dressing applications.

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Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin

Cited by 24 publications

References 45 publications

Silver distribution in chronic wounds and the healing dynamics of chronic wounds treated with dressings containing silver and octenidine

Silver distribution in chronic wounds and the healing dynamics of chronic wounds treated with dressings containing silver and octenidine

Effectiveness of local application of antiseptic with octenidin dihydrochloride molecule in smoking women with ASC-US and LSIL

3D Printing of Thermoresponsive Hydrogel Laden with an Antimicrobial Agent towards Wound Healing Applications

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