OCT Meets micro-CT: A Subject-Specific Correlative Multimodal Imaging Workflow for Early Chick Heart Development Modeling

Kraus, Nina; Placzek, Fabian; Metscher, Brian

doi:10.3390/jcdd9110379

Cited by 3 publications

(3 citation statements)

References 75 publications

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“…A little part of the inner membrane and the shell were cut away to reveal the embryo. The use of CE for subsequent imaging was avoided when the membrane bled or the CE exhibited visible structural flaws [30]. For 72 h, the retrieved embryos were preserved in a 10% buffered formalin solution.…”

Section: Embryo Preparationmentioning

confidence: 99%

High-Performance Microcomputing Tomography of Chick Embryo in the Early Stages of Embryogenesis

Rzhepakovsky,

Piskov,

Avanesyan

et al. 2023

Applied Sciences

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X-ray contrast techniques were tested on the chick embryos in early periods of embryogenesis. For contrast stain, reagents with radiopacity in various concentrations were used: silver proteinate, eosin, Lugol’s solution (I2KI), phosphomolybdic acid and phosphotungstic acid under heating at 25 °C and 40 °C and exposure for 24 and 48 h. The use of silver proteinate, eosin and I2KI in various concentrations in the contrast of the chick embryo in the early period of embryogenesis did not make it possible to obtain microtomographic results that provide reliable microstructural analysis. The most optimal and effective method of embryo staining at the HH22–HH34 embryonic stages reliably determined the staining of 1% phosphotungstic acid at 40 °C heating and exposure for 24 h. Taking into account the size of the chick embryos and their structures at the HH22–HH34 embryonic stages, the features of the development, location of organs, and the minimum permissible parameters of microtomography for obtaining high-quality and reliable results were determined by the isometric spatial resolution of 8.87 μm, X-ray voltage 50 kV, X-ray current 500 μA, and the use of filters started from Al 0.5 mm. Microtomographic results were obtained, characterized by the appearance of the chick embryo at the HH22–HH34 embryonic stages, and they visualized the locations and structures of the chick embryo organs and provided calculation of their volume and X-ray density. The results of the work open up significant prospects for using the chick embryo at the early embryonic period of embryogenesis as an alternative model for screening teratogenicity.

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Section: Embryo Preparationmentioning

confidence: 99%

High-Performance Microcomputing Tomography of Chick Embryo in the Early Stages of Embryogenesis

Rzhepakovsky,

Piskov,

Avanesyan

et al. 2023

Applied Sciences

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“…In comparison with these classical sections, newer approaches, such as ultrasound biomicroscopy, micro-MRI, and micro-computed tomography (micro-CT) provide improved resolution and image quality [ 8 , 9 ]. Micro-CT is now considered as an effective approach well suited towards rapid screening of morphological changes [ 10 – 15 ]. Micro-CT scanners collect a sequence of 2D radiographs (projections) around a radial or helical axis which are reconstructed (typically using filtered back projection or iterative reconstruction) to form a high-resolution 3D (volume) image, with voxel resolutions typically within the tens of micron to sub-micrometer range.…”

Section: Introductionmentioning

confidence: 99%

An optimized CT-dense agent perfusion and micro-CT imaging protocol for chick embryo developmental stages

Naïja,

Mutlu,

Khan

et al. 2024

BMC biomed eng

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Compared to classical techniques of morphological analysis, micro-CT (μ-CT) has become an effective approach allowing rapid screening of morphological changes. In the present work, we aimed to provide an optimized micro-CT dense agent perfusion protocol and μ-CT guidelines for different stages of chick embryo cardiogenesis. Our study was conducted over a period of 10 embryonic days (Hamburger-Hamilton HH36) in chick embryo hearts. During the perfusion of the micro-CT dense agent at different developmental stages (HH19, HH24, HH27, HH29, HH31, HH34, HH35, and HH36), we demonstrated that durations and volumes of the injected contrast agent gradually increased with the heart developmental stages contrary to the flow rate that was unchanged during the whole experiment. Analysis of the CT imaging confirmed the efficiency of the optimized parameters of the heart perfusion.

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“…Especially micro-CT (µCT) offers similar resolution and has been successfully used to validate OCT-based caries diagnostics of ex vivo tooth samples 11,12 or cardiac development models. 13 While µCT is limited to a small sample volume of several centimeters and thus not applicable for human imaging, cone-beam CT (CBCT) enables in vivo applications at the expense of resolving power. While first established in clinical dentistry, it is now widely used in the head and neck region including otolaryngology.…”

Section: Introductionmentioning

confidence: 99%

Data-informed imaging: how radiography and shape models support endoscopic OCT imaging of the middle ear

Golde

Morgenstern²,

Liu³

et al. 2023

Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2023

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Endoscopic optical coherence tomography (OCT) enables the assessment of the eardrum and the middle ear in vivo. However, revealing the ossicles is often limited due to shadowing effects of preceding structures and the 3D impression is difficult to interpret. To compare the identified middle ear structures, OCT and cone-beam CT of a patient were spatially aligned and showed a good agreement in locating malleus and the promontory wall. As CT imaging uses ionizing radiation and is thus limited in application, we furthermore provide a concept how radiology can be utilized as a priori knowledge for OCT imaging. Therefore, a statistical shape model derived from μCT data of temporal bone specimens was fitted to in vivo OCT measurements, potentially providing a real-time augmentation of endoscopic OCT for middle ear diagnostics in the future.

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OCT Meets micro-CT: A Subject-Specific Correlative Multimodal Imaging Workflow for Early Chick Heart Development Modeling

Cited by 3 publications

References 75 publications

High-Performance Microcomputing Tomography of Chick Embryo in the Early Stages of Embryogenesis

High-Performance Microcomputing Tomography of Chick Embryo in the Early Stages of Embryogenesis

An optimized CT-dense agent perfusion and micro-CT imaging protocol for chick embryo developmental stages

Data-informed imaging: how radiography and shape models support endoscopic OCT imaging of the middle ear

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