Ocorrência e diversidade estrutural de metabólitos fúngicos com atividade antibiótica

Takahashi, Jacqueline A.; Lucas, Esther Maria Ferreira

doi:10.1590/s0100-40422008000700036

Cited by 23 publications

(14 citation statements)

References 33 publications

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“…Terrestrial-derived microfungi have been the sources of several novel and pharmacologically active compounds over decades (Hormazabal et al, 2005;Schmeda-Hirschmann et al, 2008;Takahashi and Lucas, 2008). In this context, endophytes belonging to the Penicillium genus have been recognized as a rich source of bioactive secondary metabolites (Fill et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Bioactive Metabolites Produced by Penicillium sp.1 and sp.2, Two Endophytes Associated with Alibertia macrophylla (Rubiaceae)

Oliveira

Silva

Regasini

et al. 2009

Zeitschrift Für Naturforschung C

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In the course of our continuous search for bioactive metabolites from endophytic fungi living in plants from the Brazilian fl ora, leaves of Alibertia macrophylla (Rubiaceae) were submitted to isolation of endophytes, and two species of Penicillium were isolated. The acetonitrile fraction obtained in corn from a culture of Penicillium sp.1 afforded orcinol (1). On the other hand, Penicillium sp.1 cultivated in potato-dextrose-broth furnished two different compounds, cyclo-(L-Pro-L-Val) (2) and uracil (3). The chromatographic fractionation of the acetonitrile fraction obtained from Penicillium sp.2 led to three dihydroisocoumarins, 4-hydroxymellein (4), 8-methoxymellein (5) and 5-hydroxymellein (6). Compounds 5 and 6 were obtained from the Penicillium genus for the fi rst time. Additionally, metabolites 1 -6 were evaluated for their antifungal and acetylcholinesterase (AChE) inhibitory activities. The most active compounds 1 and 4 exhibited detection limits of 5.00 and 10.0 μg against Cladosporium cladosporioides and C. sphaerospermum, respectively. Compound 2 showed a detection limit of 10.0 μg, displaying potent AChE inhibitory activity.

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Section: Introductionmentioning

confidence: 99%

Bioactive Metabolites Produced by Penicillium sp.1 and sp.2, Two Endophytes Associated with Alibertia macrophylla (Rubiaceae)

Oliveira

Silva

Regasini

et al. 2009

Zeitschrift Für Naturforschung C

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“…75 Desta forma, experimentos visando o isolamento destas substâncias são geralmente longos, com duração a partir de sete dias, sendo, porém, mais comum as fermentações com três a quatro semanas de duração. A obtenção de extratos é normalmente feita utilizando-se solventes de média polaridade, como o acetato de etila e o método cromatográfico mais empregado para o isolamento de substâncias é a cromatografia líquida, podendo ser feita por adsorção, exclusão ou filtração molecular e troca iônica.…”

Section: Isolamento E Caracterização De Metabólitos Secundários Bioatunclassified

Filamentous Fungi and Chemistry: Old Friends, New Allies

Takahashi

Lima²,

Santos³

et al. 2017

Rev. Virtual Quim.

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Abstract:Fungi are classically associated with problems such as deterioration and contamination of foods, human mycoses and diseases that bring damage to agriculture. However, the biotechnological potential of these organisms surpasses enormously their potential damage and the number of fungal species with importance in chemistry, pharmacology, environment, ecology, agriculture and food is extraordinary. In this field, the chemistry helped understanding fermentation processes and fungal metabolism, increasing the use of processes using fungal metabolites. The chemistry has also helped to control the presence of fungi when they are unwanted, providing preservatives and antioxidants for food and cosmetics, antimycotic agents and antifungals for agricultural use. However, the interdisciplinarity of the fungal metabolism study has brought new and interesting challenges. This review will address important aspects on cultivation, identification, use and biotechnological potential of fungi and their metabolites from the perspective of their historical use. Then current aspects of the use of fungi for the benefit of human life, as for the development of new drugs, environmental and ecological applications such as using bioremediation, in addition to presenting new frontiers for research, as the search for fungi in new habitats, the use of challenging growing conditions for elicitation of metabolic production and the use of modern analytical techniques to enlarge the biotechnological application of fungi will be presented. Keywords:Fungi; secondary metabolites; biotransformation; bioremediation; applications. ResumoOs fungos são classicamente associados a problemas como a deterioração e contaminação de alimentos, micoses humanas e doenças que trazem prejuízos à agricultura. Entretanto, o potencial biotecnológico destes organismos supera enormemente os potenciais danos e o número de espécies fúngicas com importância química, farmacológica, ambiental, ecológica, agrícola e alimentícia é extraordinário. Neste campo, a química ajudou a entender os processos fermentativos e o metabolismo fúngico, potencializando as aplicações de processos e metabólitos fúngicos. A química também tem ajudado a controlar a presença de fungos quando a mesma é indesejada, fornecendo agentes conservantes para alimentos e cosméticos, antioxidantes, antimicóticos e antifúngicos para uso agrícola. No entanto, a interdisciplinaridade do estudo do metabolismo fúngico tem trazido novos e interessantes desafios. Nesta revisão serão abordados aspectos importantes sobre o cultivo, identificação, utilização e potencial biotecnológico de fungos e seus metabólitos partindo da perspectiva do seu uso histórico. Em seguida, serão apresentados aspectos atuais da utilização de fungos para benefício da vida humana, como o desenvolvimento de novos fármacos, aplicações ambientais e ecológicas como o uso de biorremediação, além de se apresentar novas fronteiras para a pesquisa, como a busca de fungos em novos habitats, o uso de condições de cultivo desafiadoras para e...

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“…Therefore, the use of interactive chemo-biological tools to screen suitable conditions for bioactive metabolites production is very welcome (Lam, 2007). Natural products already isolated from fungi possess numerous pharmacological uses, in special as antibacterial and antifungal agents (Takahashi and Lucas, 2008a; Takahashi et al , 2008b; Tanseer and Anjum, 2011; Newman and Cragg, 2012). Research in this area has been neglected in face of the profit perspective of some more fashioned diseases.…”

Section: Introductionmentioning

confidence: 99%

Modulation of antimicrobial metabolites production by the fungus Aspergillus parasiticus

Bracarense¹,

Takahashi²

2014

Braz. J. Microbiol.

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Biosynthesis of active secondary metabolites by fungi occurs as a specific response to the different growing environments. Changes in this environment alter the chemical and biological profiles leading to metabolites diversification and consequently to novel pharmacological applications. In this work, it was studied the influence of three parameters (fermentation length, medium composition and aeration) in the biosyntheses of antimicrobial metabolites by the fungus Aspergillus parasiticus in 10 distinct fermentation periods. Metabolism modulation in two culturing media, CYA and YES was evaluated by a 22 full factorial planning (ANOVA) and on a 23 factorial planning, role of aeration, medium composition and carbohydrate concentration were also evaluated. In overall, 120 different extracts were prepared, their HPLC profiles were obtained and the antimicrobial activity against A. flavus, C. albicans, E. coli and S. aureus of all extracts was evaluated by microdilution bioassay. Yield of kojic acid, a fine chemical produced by the fungus A. parasiticus was determined in all extracts. Statistical analyses pointed thirteen conditions able to modulate the production of bioactive metabolites by A. parasiticus. Effect of carbon source in metabolites diversification was significant as shown by the changes in the HPLC profiles of the extracts. Most of the extracts presented inhibition rates higher than that of kojic acid as for the extract obtained after 6 days of fermentation in YES medium under stirring. Kojic acid was not the only metabolite responsible for the activity since some highly active extracts showed to possess low amounts of this compound, as determined by HPLC.

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Ocorrência e diversidade estrutural de metabólitos fúngicos com atividade antibiótica

Cited by 23 publications

References 33 publications

Bioactive Metabolites Produced by Penicillium sp.1 and sp.2, Two Endophytes Associated with Alibertia macrophylla (Rubiaceae)

Bioactive Metabolites Produced by Penicillium sp.1 and sp.2, Two Endophytes Associated with Alibertia macrophylla (Rubiaceae)

Filamentous Fungi and Chemistry: Old Friends, New Allies

Modulation of antimicrobial metabolites production by the fungus Aspergillus parasiticus

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