Ochratoxin A-induced cytotoxicity, genotoxicity and reactive oxygen species in kidney cells: An integrative approach of complementary endpoints

Costa, João Guilherme; Saraiva, Nuno; Guerreiro, Patrícia S.; Louro, Henriqueta; Silva, Maria João; Miranda, Joana P.; Castro, Matilde; Batinić‐Haberle, Ines; Fernandes, Ana Sofia; Oliveira, Nuno G.

doi:10.1016/j.fct.2015.11.018

Cited by 91 publications

(75 citation statements)

References 67 publications

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“…However, the exact mechanism of OTA‐induced nephrotoxicity has not yet been completely understood. The results obtained in this study, in accordance with in vitro and in vivo studies (Abdel‐Wahhab, Aljawish, El‐Nekeety, Abdel‐Aziem, & Hassan, ; Ciarcia et al, ; Costa et al, ; Periasamy, Kalal, Krishnaswamy, & Viswanadha, ), confirmed that the toxic effect of OTA on the kidney found both on the functional level and from the histopathology of view is mediated by oxidative stress. It has been shown that OTA acts by inhibiting the nuclear factor, which would affect both the synthesis and the recycling of glutathione and also the activity of oxidoreductases thus making the tissue more vulnerable to oxidative stress (Jennings, Limonciel, Felice, & Leonard, ; Limonciel & Jennings, ).…”

Section: Discussionsupporting

confidence: 90%

Red orange and lemon extract prevents the renal toxicity induced by ochratoxin A in rats

Damiano¹,

Iovane

Squillacioti³

et al. 2020

Journal Cellular Physiology

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In this work, we investigated the effects of red orange and lemon extract (RLE) on ochratoxin A (OTA)‐induced nephrotoxicity. In particular, we analyzed the change in renal function and oxidative stress in Sprague–Dawley rats treated with OTA (0.5 mg/kg body weight, b.w.) and with RLE (90 mg/kg b.w.) by oral administration. After OTA treatment, we found alterations of biochemical and oxidative stress parameters in the kidney, related to a severe decrease of glomerular filtration rate. The RLE treatment normalized the activity of antioxidant enzymes and prevented the glomerular hyperfiltration. Histopathological examinations revealed glomerular damages and kidney cortex fibrosis in OTA‐rats, while we observed less severe fibrosis in OTA plus RLE group. Then, we demonstrated that oxidative stress could be the cause of OTA renal injury and that RLE reduces this effect.

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Section: Discussionsupporting

confidence: 90%

Red orange and lemon extract prevents the renal toxicity induced by ochratoxin A in rats

Damiano¹,

Iovane

Squillacioti³

et al. 2020

Journal Cellular Physiology

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“…Despite the numerous strategies developed to reduce OTA-induced nephrotoxicity, this mycotoxin mechanism of action remains complex and unknown. However, in recent years, several in vivo and in vitro studies have recognized that the induction of the oxidative stress induced by OTA exposure is considered one of the mechanisms responsible for its toxicity [30][31][32][33]. Thus, the administration of antioxidants that counteract oxidative stress may protect kidneys from damage.…”

Section: Discussionmentioning

confidence: 99%

Effects of Curcumin on the Renal Toxicity Induced by Ochratoxin A in Rats

et al. 2020

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Ochratoxin A (OTA) is a powerful nephrotoxin and the severity of its damage to kidneys depends on both the dose and duration of exposure. According to the scientific data currently available, the mechanism of action still is not completely clarified, but it is supposed that oxidative stress is responsible for OTA-induced nephrotoxicity. Bioactive compound use has emerged as a potential approach to reduce chronic renal failure. Therefore, curcumin (CURC), due to its therapeutic effects, has been chosen for our study to reduce the toxic renal effects induced by OTA. CURC effects are examined in Sprague Dawley rats treated with CURC (100 mg/kg), alone or in combination with OTA (0.5 mg/kg), by gavage daily for 14 days. The end result of the experiment finds rats treated with OTA show alterations in biochemical and oxidative stress parameters in the kidney, related to a decrease in the Glomerular Filtration Rate (GFR). Conversely, the administration of CURC attenuates oxidative stress and prevents glomerular hyperfiltration versus the OTA group. Furthermore, kidney histological tests show a reduction in glomerular and tubular damage, inflammation and tubulointerstitial fibrosis. This study shows that CURC can mitigate OTA–induced oxidative damage in the kidneys of rats.

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“…Cells were harvested using 5 m m EDTA in PBS at 37°C, washed with cold PBS and fixed with chilled 80% ethanol. Cells were stained with propidium iodide (10 μg/ml) in the presence of RNase A (20 μg/ml) for 15–20 min and were analysed using a FACSCalibur flow cytometer (BD Biosciences) . Data were acquired with CellQuest ® software (BD Biosciences) and analysed with FlowJo ® X.0.7 (Tree Star Inc., San Carlos, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

The APE1 redox inhibitor E3330 reduces collective cell migration of human breast cancer cells and decreases chemoinvasion and colony formation when combined with docetaxel

et al. 2017

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The human apurinic/apyrimidinic endonuclease 1 (APE1) is an ubiquitous multifunctional DNA repair enzyme and a redox signalling protein. Our work addressed the inhibition of APE1 redox function using E3330, as single agent or in combination with docetaxel (DTX), in human breast cancer MDA-MB-231 cells. E3330 decreased the colony formation of DTX-treated cells. In addition, E3330 alone significantly reduced the collective cell migration as assessed by the wound-healing assay, whereas the combined treatment decreased chemoinvasion. These results suggest that the inhibition of APE1 redox function might have therapeutic potential by modulating cell migration and invasion in metastatic breast cancer.

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Ochratoxin A-induced cytotoxicity, genotoxicity and reactive oxygen species in kidney cells: An integrative approach of complementary endpoints

Cited by 91 publications

References 67 publications

Red orange and lemon extract prevents the renal toxicity induced by ochratoxin A in rats

Red orange and lemon extract prevents the renal toxicity induced by ochratoxin A in rats

Effects of Curcumin on the Renal Toxicity Induced by Ochratoxin A in Rats

The APE1 redox inhibitor E3330 reduces collective cell migration of human breast cancer cells and decreases chemoinvasion and colony formation when combined with docetaxel

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