Occurrence, species distribution and antimicrobial resistance of thermophilic Campylobacter isolates from farm and laboratory animals in Morogoro, Tanzania

Komba, Erick; Mdegela, Robinson H.; Msoffe, Peter L. M.; Matowo, Denis E.; Maro, Makori J.

doi:10.14202/vetworld.2014.559-565

Cited by 14 publications

(19 citation statements)

References 56 publications

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“…A higher level of resistance to tetracycline among C. Jejuni isolates was reported previously in the country [75]. Recent studies in the country have however reported a moderate frequency of tetracycline resistance among thermophilic Campylobacter isolates [9,66]. In these studies however the authors reported higher resistance levels to nalidixic acid among Campylobacter isolates which is in line with this study.…”

Section: /7supporting

confidence: 90%

“…Resistance to these two antimicrobial agents was low in this study indicating that they may still be useful in the treatment of human campylobacteriosis in the country. A similar observation of low resistance to ciprofloxacin among thermophilic Campylobacter isolates was reported in a recent studies conducted in the country [9,66]. The authors however found higher frequencies of resistant isolates to erythromycin which is contrary to what we report in this study.…”

Section: /7supporting

confidence: 62%

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Contamination of Frozen Broiler Chicken Meats with Antimicrobial Resistant Thermophilic Campylobacter in Morogoro, Tanzania

Msetule¹,

Evg²,

Si³

et al. 2017

BJSTR

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Purpose: Majority of the human bacterial gastroenteritis cases in both developed and developing countries in the world are caused by thermo tolerant Campylobacter spp, with C. jejuni and C. coli being more involved. These bacteria live in poultry and other animal's intestinal tracts without causing disease symptoms. Improper handling of carcasses during slaughter and evisceration increases the chance of contaminating the outer skin. Poor meat handling in the kitchen and poor storage during refrigeration causes cross contamination. Methods:This cross-sectional study examined the occurrence of antimicrobial resistant thermophilic Campylobacter in frozen chicken meats in Morogoro Municipality. A total of 272 frozen broiler chicken carcasses were obtained from supermarkets (n=90), meat shops (n=90) and restaurants (n=92). Each carcass was divided into three parts i.e. breast, thighs and wing+neck, rinsed in peptone water. The rinses were then enriched in 5ml of Bolton broth in microerophilic atmosphere at 42ºC for 24hrs. Then the broth was streaked on Modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA) plates followed by incubation at 42ºC for 48 hrs. Presumptive colonies of Campylobacter spp. were sub cultured onto Mueller Hinton Agar and incubated at 42ºC for 48 hrs to obtain pure colonies. Pure Campylobacter colonies were then subjected to preliminary identification using biochemical tests and further confirmed by polymerase chain reaction (PCR). Antimicrobial Susceptibility testing was performed on C. Jejuni isolates by disc diffusion method on Mueller-Hinton agar supplemented with 5% of horse blood. The isolates were tested for resistance against ten antimicrobials namely tetracycline, gentamycin, ciprofloxacin, azithromycin, erythromycin, norfloxacin, chloramphenical, amoxicilin, nalidixic acid and cephalocin.Results: Occurrence of thermophilic Campylobacter spp. in the sampled birds was at a tune of 61.0%. Based on sampling locations the levels of contaminated chicken carcasses were 82.6%, 62.0% and 37.8% for restaurants, shops and supermarkets, respectively. Majority of isolates (72.4%) were Campylobacter jejuni and the remaining proportion (27.6%) was accounted for by C. coli. Frequencies of contamination were comparable between wing+neck and thighs. Wing+neck and thighs had significantly higher frequencies of contamination than breasts (P<0.05). Antibiotic resistance test results for C. jejuni indicated that higher levels (>80%) of resistance were observed for cephalothin, chloramphenical, nalidixic acid, amoxicillin and tetracycline. Lower levels of resistance (<15%) were observed for erythromycin, norfloxacin, azithromycin, ciprofloxacin and gentamicin. Conclusion:This study confirms that in our setting a high frequency of commercial broiler chickens are positive for antimicrobial resistant Campylobacter at the time of slaughter. This phenomenon derives in high contamination of carcasses during the slaughter process thereby constituting a substantial public health hazard. Freezing of carcass...

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Section: /7supporting

confidence: 90%

Section: /7supporting

confidence: 62%

Contamination of Frozen Broiler Chicken Meats with Antimicrobial Resistant Thermophilic Campylobacter in Morogoro, Tanzania

Msetule¹,

Evg²,

Si³

et al. 2017

BJSTR

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show abstract

“…Colonization with the organism was detected in 26.7% of the guinea pigs and 1.2% of the rats [72]. In the same country colonization of horses with thermophilic Campylobacter was detected at a tune of 60% (n=5) [72].…”

Section: Thermophilic Campylobacter In Non Food and Laboratory Animalsmentioning

confidence: 94%

“…A number of studies in East African countries have addressed the issue of antimicrobial resistance in the organisms. In Tanzania studies on both animals [70,72] …”

Section: Antimicrobial Resistance Profiles Of Campylobacter Isolatesmentioning

confidence: 99%

Human and Animal Thermophilic Campylobacter infections in East African countries: Epidemiology and Antibiogram

Komba¹

2017

BJSTR

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“…Studies among laboratory research animals have found guinea pigs to carry Campylobacter spp. (Weber et al, ; Muto et al, ; Fakir, ; Bartholomew et al, ; Komba et al, ). In the Andean Region, guinea pigs have been found to carry important zoonotic pathogens such as Fasciola hepatica (Carolina Gonzalez et al ., ) and Yersinia pestis , (Gabastou et al, ), as well as Trypanosoma cruzi (Levy et al, ).…”

Section: Introductionmentioning

confidence: 99%

Hyperendemic Campylobacter jejuni in guinea pigs (Cavia porcellus) raised for food in a semi‐rural community of Quito, Ecuador

Graham

Vasco

Trueba

2016

Environ Microbiol Rep

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Summary Domestic animals and animal products are the source of pathogenic Campylobacter jejuni and C. coli in industrialized countries, yet little is known about the transmission of these bacteria in developing countries. Guinea pigs (Cavia porcellus) are commonly raised for food in the Andean region of South America, however, limited research has characterized this rodent as a reservoir of zoonotic enteric pathogens. In this study, we examined the prevalence of Campylobacter spp. in 203 fecal samples from domestic animals of 59 households in a semi-rural parish of Quito, Ecuador. Of the twelve animal species studied, guinea pigs showed the highest prevalence of C. jejuni (n = 39/40; 97.5%). Multilocus sequence typing (MLST) was used to characterize the genetic relationship of C. jejuni from domestic animals and 21 sequence types (STs) were identified. The majority of STs from guinea pigs appeared to form new clonal complexes that were not related to STs of C. jejuni isolated from other animal species and shared only a few alleles with other C. jejuni previously characterized. The study identifies guinea pigs as a major reservoir of C. jejuni and suggests that some C. jejuni strains are adapted to this animal species.

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Occurrence, species distribution and antimicrobial resistance of thermophilic Campylobacter isolates from farm and laboratory animals in Morogoro, Tanzania

Cited by 14 publications

References 56 publications

Contamination of Frozen Broiler Chicken Meats with Antimicrobial Resistant Thermophilic Campylobacter in Morogoro, Tanzania

Contamination of Frozen Broiler Chicken Meats with Antimicrobial Resistant Thermophilic Campylobacter in Morogoro, Tanzania

Human and Animal Thermophilic Campylobacter infections in East African countries: Epidemiology and Antibiogram

Hyperendemic Campylobacter jejuni in guinea pigs (Cavia porcellus) raised for food in a semi‐rural community of Quito, Ecuador

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