Occurrence, prevalence and seasonality of neoplasia in the marine mussel Mytilus edulis from three sites in Denmark

Rasmussen, Lars P.D.

doi:10.1007/bf00392746

Cited by 33 publications

(26 citation statements)

References 11 publications

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“…The ultrastructural characteristics of the neoplastic cells observed in the Galician diseased cockles closely resembled those of neoplastic cells from Cerastoderma edule from Brittany and Ireland (Collins 1998). Moreover, the presence of large nuclei, hypertrophied mitochondria and atypical Golgi complexes correspond to the ultrastructure features of neoplastic cells described from Macoma balthica (Farley 1976, Smolarz et al 2006, Mya arenaria (Brown et al 1977) and Mytilus edulis (Mix et al 1979, Green & Alderman 1983, Rasmussen 1986) affected by DN. The abundance of mitochondria in neoplastic cells could be related to the high quantity of energy required for cell division (Smolarz et al 2006).…”

Section: Discussionsupporting

confidence: 67%

Disseminated neoplasia in cockles Cerastoderma edule: ultrastructural characterisation and effects on haemolymph cell parameters

Díaz

Renault²,

Villalba³

et al. 2011

Dis. Aquat. Org.

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Disseminated neoplasia (DN) has been detected in cockles from various beds in Galicia (NW Spain). A study was performed to characterise cockle neoplastic cell ultrastructure and to evaluate the effect of this disease at different severity stages on various haemolymph cell parameters. Examination of cockle neoplastic cells with transmission electron microscopy (TEM) showed round shapes and a lack of pseudopods, a high nucleus:cytoplasm diameter ratio, Golgi complexes, abundant mitochondria, ribosomes, and numerous endoplasmic reticulum tubes and electron-lucent vesicles. Various haemolymph cell parameters (cell mortality, non-specific esterase and lysosome biovolume, reactive oxygen intermediates [ROI] production, phagocytosis ability, intracellular Ca 2+ and actin levels) were compared between DN severity categories by flow cytometry; haemocyte mortality, non-specific esterase activities and lysosome biovolume were found to be higher with increasing DN severity. The phagocytic ability of neoplastic cells was sharply reduced with regard to haemocytes. The cytoplasmic-free Ca 2+ level was higher and actin content lower in haemolymph cells of diseased cockles compared to unaffected ones. A significant increase in ROI production was detected in later stages of disease progression.KEY WORDS: Disseminated neoplasia · Cerastoderma edule · Non-specific esterases · Lysosome · Respiratory burst · Phagocytosis · Cytoplasmic free Ca 2+ · Actin Resale or republication not permitted without written consent of the publisherDis Aquat Org 96: 157-167, 2011 158 tected in cockles from various beds in Galicia (NW Spain) and its association with cockle mortality was suggested in natural beds (Carballal et al. 2001, Villalba et al. 2001. Significantly higher mortality of DNaffected cockles than of unaffected ones has been confirmed in laboratory tanks (S. Díaz unpubl. data).Various studies have shown the loss of phagocytosis ability of neoplastic cells in comparison with normal haem ocytes in Mytilus trossulus (Kent et al. 1989, Noël 1992, M. edulis (Galimany & Sunila 2008) and Mya arenaria , Potts 1993. Analyses by light microscopy showed failures in adhesion and spreading on glass slides of neoplastic cells in M. edulis (Elston et al. 1988). Loss of adhesion ability could be the result of an alteration of the cell surface proteins. Normal haemocytes of M. arenaria express a 130 KDa protein recognised by murine monoclonal antibody MAB 2A4 that is not detected in neoplastic cells (Miosky et al. 1989), which may be related to adherence (White et al. 1993). Differences in adherence and phagocytic behaviour between haemocytes and neoplastic cells are related to altered cytoskeletal structure. Significant differences in the cytoskeletal architecture and quantity of actin filaments were found between haemocytes and abnormal cells of M. arenaria .Lysosomal enzymes and reactive oxygen intermediates (ROIs) are immune factors used by haemocytes during phagocytosis to destroy microorganisms (Cheng 1981). Hydrolytic enzymes were ...

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Section: Discussionsupporting

confidence: 67%

Disseminated neoplasia in cockles Cerastoderma edule: ultrastructural characterisation and effects on haemolymph cell parameters

Díaz

Renault²,

Villalba³

et al. 2011

Dis. Aquat. Org.

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“…Neoplasia has been detected in M. edulis in the UK in the Irish Sea (4) and the North Sea (5) (Lowe & Moore 1978, Green & Alderman 1983 and in M. trossulus in the Baltic Sea (6), Denmark by Rasmussen (1986) and in Finland by Sunila (1987). In North America , in the West Atlantic, Figueras et al (1991b) reported a case of disseminated neoplasia in M. edulis in Maine, USA, and Hillman et al (1992) reported seven cases during six years of the Mussel Watch Program among several thousand mussels studied in Massachusetts, Connecticut and New York (7).…”

Section: Discussionmentioning

confidence: 99%

Disseminated neoplasia in blue mussels, Mytilus galloprovincialis, from the Black Sea, Romania

Ciocan

Sunila

2005

Marine Pollution Bulletin

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“…Farley (1969) first suggested an origin for neoplastic cells in the 'primordial epithelium of the gonad' after noting the morphological resemblance among undifferentiated germ cells, normal hyaline hemocytes, and neoplastic cells in eastern oysters. Rasmussen (1986) later pointed out the similarity between focal development of both germinal and neoplastic cells in vesicular connective tissue of Mytilus edulis and also suggested a gonadal origin for the latter. We were unable to identify an origin for the neoplastic cells from tissue slide examination; hotvever, the striking temporal correlation between the prevalence of neoplasms and degree of gametogenic activity in our study may offer some support for the gonadal origin hypothesis.…”

Section: Gametogenesismentioning

confidence: 98%

Disseminated neoplasia in juvenile eastern oysters Crassostrea virginica, and its relationship to the reproductive cycle

Se¹,

Rd²,

Marks³

1997

Dis. Aquat. Org.

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Occurrence, prevalence and seasonality of neoplasia in the marine mussel Mytilus edulis from three sites in Denmark

Cited by 33 publications

References 11 publications

Disseminated neoplasia in cockles Cerastoderma edule: ultrastructural characterisation and effects on haemolymph cell parameters

Disseminated neoplasia in cockles Cerastoderma edule: ultrastructural characterisation and effects on haemolymph cell parameters

Disseminated neoplasia in blue mussels, Mytilus galloprovincialis, from the Black Sea, Romania

Disseminated neoplasia in juvenile eastern oysters Crassostrea virginica, and its relationship to the reproductive cycle

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