Disseminated neoplasia in juvenile eastern oysters Crassostrea virginica, and its relationship to the reproductive cycle

Se, Ford; Rd, Barber; Marks, Edwin P.

doi:10.3354/dao028073

Cited by 16 publications

(18 citation statements)

References 9 publications

(9 reference statements)

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“…Potts (1996) reported for M. arenaria that, as DN advanced, the gonadal follicles became significantly smaller in size but were not reduced in number. Similar results were found for C. virginica, where most of the oysters with neoplasia had measurable gonads, but their gonadal indices (proportion of visceral mass area occupied by gonad) were generally below the mean for non-neoplastic individuals (Ford et al, 1997). Cremonte et al (2011) reported gonadal atrophy in mussels M. chilensis affected by advanced stages of DN.…”

Section: Sublethal Effectssupporting

confidence: 85%

“…The occurrence of high levels of DN in a single year class of juvenile oysters C. virginica was associated with exceptionally heavy setting of that year class, resulting in extreme crowding (Ford et al, 1997). This association reinforces the hypothesis of a transmissible agent being responsible for DN (see Section 2.5.1).…”

Section: Other Factorsmentioning

confidence: 70%

“…Nevertheless, none of these studies support their discussion with correlations between prevalence and condition/gametogenic indices or with observational data derived from histology. Ford et al (1997) described a prevalence pattern of DN following that of gametogenesis in a single year class of juvenile oysters C. virginica surveyed histologically in lower Delaware Bay (New Jersey, USA); a first peak of DN co-occurred with the period of maximum gonadal development and a decline with the first sign of spawning; the second neoplasia peak occurred after the major spawning. An identical pattern could be observed in Fig.…”

Section: Determinant Factors On the Occurrence Of Disseminated Neoplasiamentioning

confidence: 98%

See 2 more Smart Citations

Neoplastic diseases of marine bivalves

Carballal

Barber

Iglesias

et al. 2015

Journal of Invertebrate Pathology

116

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Section: Sublethal Effectssupporting

confidence: 85%

Section: Other Factorsmentioning

confidence: 70%

Section: Determinant Factors On the Occurrence Of Disseminated Neoplasiamentioning

confidence: 98%

See 1 more Smart Citation

Neoplastic diseases of marine bivalves

Carballal

Barber

Iglesias

et al. 2015

Journal of Invertebrate Pathology

116

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“…Some authors (Farley, 1969;Ford et al, 1997;Rasmussen, 1986) suggested a possible gonadal origin of DN, which could explain these results. However, other authors favor a hemopoietic origin (Balouet et al, 1986;Elston et al, 1992).…”

Section: Discussionmentioning

confidence: 80%

Evidence of retroviral etiology for disseminated neoplasia in cockles (Cerastoderma edule)

Romalde

Vilariño

Beaz

et al. 2007

Journal of Invertebrate Pathology

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“…Environmental carcinogens such as hydrocarbons (Yevich & Barszcz 1977, Naes et al 1995, herbicides (van Beneden et al 1993), biotoxins (Landsberg 1996, Roy et al 1998) and infectious agents (Farley et al 1972, Ford et al 1997 have been suggested as the main risk factors for disseminated neoplasia. Research on mollus-can neoplasia has been motivated by the question as to whether bivalves can be used as bioindicators in environmental monitoring (Roy et al 1998, Brousseau et al 2000, Fournier et al 2001, Sauve et al 2002.…”

Section: Introductionmentioning

confidence: 99%

Neoplasia detection in Macoma balthica from the Gulf of Gdansk: comparison of flow cytometry, histology and chromosome analysis

Smolarz¹,

Renault²,

Soletchnik³

et al. 2005

Dis. Aquat. Org.

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A flow cytometry protocol was applied for the detection of neoplasia in Macoma balthica L. from the Gulf of Gdansk (Baltic Sea, Poland). A simple method, based on an osmotic shock, was used to permeabilise gill cells. The cytometric pattern of normal clams consisted of 2 peaks, a major peak B and a smaller peak C. The cytometric pattern of affected clams consisted of 2 peaks named B' and C'. Two parameters were used to define the stages of abnormalities in M. balthica clams based on the percentage of cells in peaks B, C, B' and C' and on the ratio between the fluorescence value of peaks B, C, B' and C' in all individuals. Three stages of neoplasia were clearly distinguished by flow cytometry considering peak C'. Stage 1 was characterised by a major population of cells in peak B' and more than 10% of cells in the C' peak. Stage 2 consisted of a lower percentage of cells in peak B' and more than 25% of cells in peak C'. Stage 3 of the neoplasia was characterised by a further reduction in peak B' and more than 40% of cells in peak C'. Flow cytometry allowed for objective detection of neoplasia and provided a rapid method for measuring the DNA content of thousands of cells per individual. The accuracy of flow cytometry was assessed by comparing with standard histological techniques, used here as a reference technique for the detection of neoplasia, and with chromosome analysis. All individuals were analysed in parallel using the 3 techniques. The proportion of normal and affected individuals diagnosed using flow cytometry was comparable to the proportion determined by histology and chromosome analysis. KEY WORDS: Flow cytometry · Histology · Cytogenetics · Neoplasia · Macoma balthica · Baltic Sea Resale or republication not permitted without written consent of the publisherDis Aquat Org 65: [187][188][189][190][191][192][193][194][195] 2005 can neoplasia has been motivated by the question as to whether bivalves can be used as bioindicators in environmental monitoring (Roy et al. 1998, Brousseau et al. 2000, Fournier et al. 2001, Sauve et al. 2002.High prevalence of neoplasia can be found in Macoma balthica L. sampled from industrialised waterways around the Gulf of Gdansk in Poland (Thiriot-Quiévreux & Wolowicz 1996). Clams at these sites are known to be exposed to a range of chemical carcinogens and pollutants (Wolowicz 1994, Szefer et al. 1996, Sokolowski et al. 1999, Hummel et al. 2000. Neoplasia in M. balthica have also been reported from the Chesapeake Bay (Christensen et al. 1974) and Finnish coast (Pekkarinen 1993).This report describes a flow cytometry protocol for the detection of gill neoplasia in Macoma balthica from the Gulf of Gdansk (Baltic Sea, Poland). Flow cytometry has already been used to investigate disseminated neoplasia in molluscs (Elston et al. 1990, Moore et al. 1991, Reno et al. 1994. In the present study, a new method of cell lysis based on a hyposmotic shock has been developed and a combined comparison of the flow cytometry method, histology and chromosome analysis is reporte...

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Disseminated neoplasia in juvenile eastern oysters Crassostrea virginica, and its relationship to the reproductive cycle

Cited by 16 publications

References 9 publications

Neoplastic diseases of marine bivalves

Neoplastic diseases of marine bivalves

Evidence of retroviral etiology for disseminated neoplasia in cockles (Cerastoderma edule)

Neoplasia detection in Macoma balthica from the Gulf of Gdansk: comparison of flow cytometry, histology and chromosome analysis

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