Occurrence ofPenicillium marneffei infections among wild bamboo rats in Thailand

Ajello, Libero; Padhye, A. A.; Sukroongreung, S; Nilakul, C; Tantimavanic, S.

doi:10.1007/bf01103897

Cited by 66 publications

(50 citation statements)

References 40 publications

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“…These findings corroborate those already described in the literature and show the importance of this animal group as reservoirs of several pathogens (46). Some rodent species are considered reservoirs of Coccidioides immitis and Penicillium marneffei; similarly, the rodents Calomys callosus have been recently described as susceptible to experimental infection with P. brasiliensis (47)(48)(49)(50).…”

Section: Discussionsupporting

confidence: 80%

Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens

Richini-Pereira¹,

Bosco²,

Theodoro³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Road-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several fungi, as well as determining natural reservoirs in wild animals and facilitating the understanding of host-pathogen relationships.

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Section: Discussionsupporting

confidence: 80%

Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens

Richini-Pereira¹,

Bosco²,

Theodoro³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Other bamboo rat isolates were gifts from Samaniya Sukroongreung, Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand. These isolates were obtained in 1987 (1). The isolates were recovered from different internal organs of one bamboo rat and displayed the same macrorestriction pattern.…”

mentioning

confidence: 99%

“…In addition to the infection in humans, P. marneffei was also reported to be isolated from the internal organs of the bamboo rat. In Thailand, the first survey was carried out in the central plains from June to September 1987 (1). Thirty-one small bamboo rats (Cannomys badius) and eight hoary bamboo rats (Rhizomys pruinosus) were trapped.…”

mentioning

confidence: 99%

Molecular Typing of Penicillium marneffei Isolates from Thailand by Not I Macrorestriction and Pulsed-Field Gel Electrophoresis

et al. 2001

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Penicillium marneffei is recognized as one of the most frequently detected opportunistic pathogens of AIDS patients in northern Thailand. We undertook a genomic epidemiology study of 64 P. marneffei isolates collected from immunosuppressed patients by pulsed-field gel electrophoresis (PFGE) with restriction enzyme NotI. Among the 69 isolates fingerprinted by PFGE, 17 were compared by HaeIII restriction endonuclease typing. The PFGE method demonstrated a higher degree of discriminatory power than restriction endonuclease typing with HaeII. Moreover, an impressive diversity of P. marneffei isolates was observed, as there were 54 distinct macrorestriction profiles among the 69 isolates of P. marneffei. These profiles were grouped into two large clusters by computer-assisted similarity analysis: macrorestriction pattern I (MPI) and MPII, with nine subprofiles (MPIa to MPIf and MPIIa to MPIIc). We observed no significant correlation between the macrorestriction patterns of the P. marneffei isolates and geographical region or specimen source. It is interesting that all isolates obtained before 1995 were MPI, and we found an increase in the incidence of infections with MPII isolates after 1995. We conclude that PFGE is a highly discriminatory typing method and is well suited for computer-assisted analysis. Together, PFGE and NotI macrorestriction allow reliable identification and epidemiological characterization of isolates as well as generate a manageable database that is convenient for expansion with information on additional P. marneffei isolates.

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“…In nature, P. marneffei has been isolated from four species of bamboo rats, as well from soil and feces samples obtained from bamboo rat burrows (1). When case-control studies were performed to identify risk factors for P. marneffei infections, neither exposure to, nor consumption of, bamboo rats was identified as a significant risk factor for infection (11).…”

mentioning

confidence: 99%

Analysis of Polymorphic Microsatellite Markers for Typing Penicillium marneffei Isolates

Lasker

Ran

2004

J Clin Microbiol

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Penicillium marneffei is an emerging opportunistic dimorphic fungal pathogen that is endemic in Southeast Asia. A typing method based on the analysis of size polymorphisms in microsatellite loci was investigated. Three loci available from the GenBank database were identified to harbor microsatellites. PCR primers flanking the microsatellite repeats were designed with one primer in the set fluorescently labeled. PCR products were then sized by automated capillary electrophoresis. As expected for a haploid fungus, a single band was observed for each microsatellite locus for all isolates. Polymorphic microsatellite marker (PMM) analysis detected a total of 22 different allelic types for 35 isolates of P. marneffei with a high discriminatory power (D ‫؍‬ 0.956). Microsatellites I, II, and III detected 14, 10, and 7 alleles, respectively. The reproducibility of length polymorphisms was confirmed by using different DNA preparations from the same isolate or by repeated runs from the same DNA preparation. PMM profiles for eight isolates passaged in vitro for 7 to 8 weeks were identical to the original culture, demonstrating short-term stability and reproducibility. PCR products were not observed for other dimorphic fungi or human DNA. Comparison of allelic frequencies in isolates obtained from China and Thailand identified distinct allele combinations, suggesting the potential geographic isolation of populations. Due to the high discriminatory power, reproducibility, and potential for high throughput, PMM analysis may provide a good typing method for epidemiologic and surveillance investigations of P. marneffei.

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Occurrence ofPenicillium marneffei infections among wild bamboo rats in Thailand

Cited by 66 publications

References 40 publications

Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens

Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens

Molecular Typing of Penicillium marneffei Isolates from Thailand by Not I Macrorestriction and Pulsed-Field Gel Electrophoresis

Analysis of Polymorphic Microsatellite Markers for Typing Penicillium marneffei Isolates

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