Occurrence of Virulence-Associated Properties in<i>Enterobacter cloacae</i>

Keller, Rogéria; Pedroso, Margareth Zabeu; Ritchmann, Rosana; Silva, Rosa Maria

doi:10.1128/iai.66.2.645-649.1998

Cited by 98 publications

(41 citation statements)

References 42 publications

(44 reference statements)

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“…The prevalence of E. coli from cloacae in this study is in line with the privies studies [14,15]. In contrast the finding of this prevalence in the present study is higher than the study of [16,22,23,24 ] in which E. coli was isolated from cloacae samples taken from poultry farms. In another study, [25,26] In the present study, all of the E. coli isolates were susceptible to Kanamycin, Trimethoprim, Ciprofloxacin and Chloramphenicol is in line with the work of Hiko et al ., 2008.…”

Section: Isolation and Identification Of E Colisupporting

confidence: 88%

Isolation and antimicrobial susceptibility pattern of Escherichia Coli from laying chicken and egg in Jimma Town, Ethiopia

Taddese¹,

Shumi²,

Asefa³

2020

Preprint

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Objectives Food borne diseases are one of the main important issues in the world. Escherichia coli are considered as the most prevalent food borne pathogen. A cross sectional study was conducted from January 6- 2018 to September 10-2019 on cloacae swab; farm and market’s egg at Jimma town to isolate, identify and determine antimicrobial susceptibility patterns. Result Out of 415 total samples, 156 (37.59%) were identified as E. coli from farm eggshell 21/83 (25.30%), farm egg content 12/83 (14.46%), cloacae swab 42/83 (50.60%) and market egg shell 45/83 (54.23%). In the current study antimicrobial susceptibility test of E. coli isolates from different sample types revealed varying degree of susceptibility to antimicrobial. Isolated E. coli was highly susceptible to Ciprofloxacin (100%), Gentamicine (100%), Streptomycin ( 96.4% ) , sulfonamides (94%), Kanamycin (91%), Chloramphenicol ( 89.5% ) , tetraccine (78.9%) , Trimethoprim (75%) these are considered appropriate for empirical treatment of E. coli in the study area. Moreover, resistance of isolates with 100%, 75% 50%, 25%, 1.6% and 0.5% was developed to ampicillin, neomycin, cefoxitin oxytetracycline , Streptomycin and tetraccine respectively. The presence of E.coli within egg/and chicken can pose serious public health problems. The appropriate hygienic practices of eggs at both farms and markets should be under taken.

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Section: Isolation and Identification Of E Colisupporting

confidence: 88%

Isolation and antimicrobial susceptibility pattern of Escherichia Coli from laying chicken and egg in Jimma Town, Ethiopia

Taddese¹,

Shumi²,

Asefa³

2020

Preprint

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“…Investigation of this supposition will require isolation of the organism inhabiting the rumen wall in order to allow for further definition of its biochemical characteristics. On the other hand, the detection of E. cloacae, which is an important cause of nosocomial infections (Keller et al 1998), both in the rumen fluid and on the rumen wall shows a possibility that the rumen is a reservoir for the organism. Further work is needed to clarify the physiological and nutritional functions of the microbial communities on the rumen wall and the relationship between E. cloacae inhabiting the rumen and public health.…”

Section: Resultsmentioning

confidence: 99%

Detection of Proteobacteria from the rumen by PCR using methanotroph-specific primers

Mitsumori

Ajisaka²,

Tajima

et al. 2002

Lett Appl Microbiol

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Aims: To detect Proteobacteria, including methanotrophs, from the rumen fluid and the bacteria inhabiting the rumen epithelium. Methods and Results: Proteobacteria inhabiting the rumen were detected by PCR using methanotroph-specific primers. The detected Proteobacteria were divided into clusters A, B, and C in addition to one clone, which was distinct from the clusters and closely related to Nitrosomonas sp. The clusters A, B, and C were close to Succinivibrio dextrinosolvens, Enterobacter cloacae, and Actinobacillus minor, respectively. The clones obtained from the rumen fluid each belonged to cluster A or B. The clones obtained from the rumen epithelium belonged to cluster B or C or to Nitrosomonas sp. Conclusions: It has been assumed that the rumen fluid and the rumen epithelium host different populations of Proteobacteria. Moreover, detection of Nitrosomonas from the rumen epithelium would indicate the possibility that the bacterium oxidizes ammonia and methane on the rumen surface. Significance and Impact of the Study: These findings suggest that the rumen fluid and the epithelium support different microbial populations, which would play specific roles in rumen function. Future study should focus on the relationship between these communities and physiological functions in the rumen.

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“…The bacteria were successfully modified/manipulated and the genes producing the two proteins, defensin and red fluorescence/ fluorescent, were added to the Bacteria genome. Enterobacter cloacae are natural human and animal digestive bacteria and are not considered as a primary pathogen [24]. This Bacterium is found in water, sewage and soil [25].…”

Section: Discussionmentioning

confidence: 99%

Dynamics and Fitness Cost of Genetically Engineered Entrobacter cloacae Expressing Defensin for Paratransgenesis in Phlebotomus papatasi

Abassi¹,

Akhlaghi²,

Oshaghi³

et al. 2019

J Bacteriol Parasitol

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Background: Enterobacter cloacae subsp. dissolvens bacterium is a known commensal of the gut microflora of Phlebotomus papatasi, the main vector for zoonotic cutaneous leishmaniasis, and nominated for paratransgenesis in sand flies. In this study, we evaluated dynamics and fitness costs of engineered E. cloacae for its potential to serve as a 'Trojan Horse' in P. papatasi. Methods: The engineered strain of E. cloacae transformed with a constantly active expressed red fluorescent protein plus defensin (EC-DR) plasmid and was fed to sand fly colonies via larval food to larvae. A wild type the bacterium (EC-WT) and intact food were used as controls. Fitness characters as well as dynamics of the EC-DR at various development stages of sand fly larvae were tested by plating homogenized specimens and counting fluorescent expressing colonies on the Tet-BHI agar medium. Results: Enterobacter cloacae DR producing red fluorescent protein could be isolated from the larvae gut after 36 days when the bacteria were added once in larval pots. The EC-DR with multiple applications had no negative effect on emergence time of instar II larvae, pupae, and adults but increased slightly mortality rate of P. papatasi larvae. The experiment also confirmed lack or weak trans-stadial transmission of E. cloacae DR in P. papatasi. It has minimal fitness cost on P. papatasi feeding behavior and survival. Conclusion: Results of this study showed that E. cloacae DR is suitable for paratransgenesis of P. papatasi at only adult stage because it did not transmit transstadially.

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Occurrence of Virulence-Associated Properties inEnterobacter cloacae

Cited by 98 publications

References 42 publications

Isolation and antimicrobial susceptibility pattern of Escherichia Coli from laying chicken and egg in Jimma Town, Ethiopia

Isolation and antimicrobial susceptibility pattern of Escherichia Coli from laying chicken and egg in Jimma Town, Ethiopia

Detection of Proteobacteria from the rumen by PCR using methanotroph-specific primers

Dynamics and Fitness Cost of Genetically Engineered Entrobacter cloacae Expressing Defensin for Paratransgenesis in Phlebotomus papatasi

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