Occurrence of vancomycin-resistant enterococci in humans and animals in the Czech Republic between 2002 and 2004

Kolář, Milan; Pantůček, Roman; Bardoň, Jan; Čekanová, Luboslava; Kesselová, Michaela; Šauer, Pavel; Vágnerová, Iva; Koukalová, Dagmar

doi:10.1099/jmm.0.45824-0

Cited by 11 publications

(6 citation statements)

References 12 publications

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“…The results of the current study were similar to the study of [10], who identified 9 isolates (1.1%) as vancomycin-resistant Enterococci (VRE), two of them had the VanA gene, one wasolate had the VanB gene, and six wasolates had The VanC gene. [11] conducted a study of the genetic content of isolates of Staphylococcus spp.…”

Section: Discussionsupporting

confidence: 87%

Molecular detection of some Vancomycin Pathogenic bacteria in Basrah Governorate

Thamer

Shareef

2022

Basrah Researches Sciences

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The current study aimed to study the resistance of some species Bacterial resistance to the antibiotic vancomycin. 121 samples were collected and grown on Chocolate Agar medium for isolating pathogenic bacteria. and then It was grown on Nutrient Agar medium for pure colonies. These bacteria were diagnosed using phenotypic methods, biochemical tests, and 16SrRNA gene. The results reported that 40 (33.3%) resistant strains appeared, of which 13 were males with a percentage of (32.5%), while the number of female samples was 27 with a percentage (67.5%) and the rest were sensitive to this antibiotic. done during the study The current study obtained four types of bacteria resistant to vancomycin: 24 strains (60%) belonging to Pseudomonas aeruginosa bacteria, 11 strains (27.5%) belonging to Proteus mirabilis bacteria, 4 strains (10%) of Staphylococcus aureus bacteria, and one strain (2.5%) for Actinomyces sp. As for the presence of resistance genes, Vancomycin, where the current study recorded the presence of 17 bacterial strains containing both VanA and VanB genes (42.5%), while the VanA gene was present in 8 strains (20%), and the VanB gene was present in 6 strains (15%), and 9 appeared Strains (22.5%) do not possess either of the two genes (VanA and VanB), although they were resistant to vancomycin, and this may be due to their possession of other resistance genes or other mechanisms of resistance to this antibiotic.

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Section: Discussionsupporting

confidence: 87%

Molecular detection of some Vancomycin Pathogenic bacteria in Basrah Governorate

Thamer

Shareef

2022

Basrah Researches Sciences

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“…), Czech Republic (Kolar et al . ) and Saudi Arabia (Qadri and Postle ). The highest documented VRE colonization rates occur in Palestine (Hijazi et al .…”

Section: Discussionmentioning

confidence: 99%

Characterization and risk factors of vancomycin-resistantEnterococci(VRE) among animal-affiliated workers in Malaysia

et al. 2012

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Aims: This study determined the risk factors and characteristics of vancomycin-resistant Enterococci (VRE) among individuals working with animals in Malaysia. Methods and Results: Targeted cross-sectional studies accompanied with laboratory analysis for the identification and characterization of resistance and virulence genes and with genotype of VRE were performed. VRE were detected in 9Á4% (95% CI: 6Á46-13Á12) of the sampled populations. Enterococcus faecium, Enterococcus faecalis and Enterococcus gallinarum were isolated, and vanA was detected in 70% of the isolates. Enterococcus faecalis with vanB was obtained from one foreign poultry worker. At least one virulence gene was detected in >50% of Ent. faecium and Ent. faecalis isolates. The esp and gelE genes were common among Ent. faecium (58Á3%) and Ent. faecalis (78%), respectively. The VRE species showed diverse RAPD profiles with some clustering of strains based on the individual's background. However, the risk factors found to be significantly associated with the prevalence of VRE were age (OR: 5Á39, 95% CI: 1Á98-14Á61) and previous hospitalization (OR: 4Á06, 95% CI: 1Á33-12Á35). Conclusion: VRE species isolated from individuals in this study have high level of vancomycin resistance, were genetically diverse and possessed the virulence traits. Age of individuals and history of hospitalization rather than occupational background determined VRE colonization. Significance and Impact of the Study: This study provides comprehensive findings on the epidemiological and molecular features of VRE among healthy individuals working with animals.

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“…faecium carrying the vanA gene followed by Ent. faecalis with vanB (Kolar et al 2002(Kolar et al , 2005(Kolar et al , 2006. Information on VRE in wildlife is limited.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons

Radiměřský

Frolková

Janoszowská

et al. 2010

Journal of Applied Microbiology

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Aims: To determine the presence of antibiotic‐resistant faecal Escherichia coli and Enterococcus spp. in feral pigeons (Columba livia forma domestica) in the Czech Republic. Methods and Results: Cloacal swabs of feral pigeons collected in the city of Brno in 2006 were cultivated for antibiotic‐resistant E. coli. Resistance genes, class 1 and 2 integrons, and gene cassettes were detected in resistant isolates by polymerase chain reaction (PCR). The samples were also cultivated for enterococci. Species status of enterococci isolates was determined using repetitive extragenic palindromic‐PCR. Resistance genes were detected in resistant enterococci by PCR. E. coli isolates were found in 203 of 247 pigeon samples. Antibiotic resistance was recorded in three (1·5%, nE. coli = 203) isolates. Using agar containing ciprofloxacin, 12 (5%, nsamples = 247) E. coli strains resistant to ciprofloxacin were isolated. No ESBL‐producing E. coli isolates were detected. A total of 143 enterococci were isolated: Ent. faecalis (36 isolates), Ent. faecium (27), Ent. durans (19), Ent. hirae (17), Ent. mundtii (17), Ent. gallinarum (12), Ent. casseliflavus (12) and Ent. columbae (3). Resistance to one to four antibiotics was detected in 45 (31%) isolates. Resistances were determined by tetK, tetL, tetM, tetO, aac(6′)aph(2′′), ant(4′)‐Ia, aph(3′)‐IIIa, ermB, pbp5, vanA and vanC1 genes. Conclusions: Antibiotic‐resistant E. coli and Enterococcus spp. occurred in feral pigeons in various prevalences. Significance and Impact of the Study: Feral pigeon should be considered a risk species for spreading in the environment antimicrobial resistant E. coli and enterococci.

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Occurrence of vancomycin-resistant enterococci in humans and animals in the Czech Republic between 2002 and 2004

Cited by 11 publications

References 12 publications

Molecular detection of some Vancomycin Pathogenic bacteria in Basrah Governorate

Molecular detection of some Vancomycin Pathogenic bacteria in Basrah Governorate

Characterization and risk factors of vancomycin-resistantEnterococci(VRE) among animal-affiliated workers in Malaysia

Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons

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