Antibiotics resistant bacteria have become a global problem as a result of the unprogrammed use of antibiotics, resulting in bacterial strains resistant to many antibiotics, or to all available antibiotics. Plants are a good source of primary and secondary metabolites that have a major role in reducing silver nitrate to silver nanoparticles (AgNPs). The production of these nanoparticles were carried out by using aqueous extract of Carthamus oxycantha M.Bieb. This can be verified by color change of the reaction solution from yellow to dark brown because of the excitation of the surface plasmon resonance. AgNPs were characterized by UV-Vis spectroscopy, where they recorded the peak at 420 nm. Fourier Transformation-infrared (FTIR) was conducted to identify the effective plant group that contributes to the formation of AgNPs and it was found that proteins and phenols have the major role in the formation of those nanoparticles. Shapes and sizes of the synthesized AgNPs were characterized by Scanning Electron Microscope (SEM) with a range of 50-80nm in size and spherical in shapes. Antibacterial activity of AgNPs were tested against Multi-Drug Resistant bacteria (MDR), Extremely antibiotics Resistant (XDR), and Pan drug-resistant (PAN) bacteria, was done in concentrations ranging from 1000-63 µg/ml. The results showed that there were significant variations between the concentrations, the tested bacteria also showed significant differences in its sensitivity to AgNPs. The results recorded a proportional relation between the type of bacterial resistance to antibiotics and it's resistant to AgNPs, therefore the most resistant bacteria to AgNPs in this study Enterobacter cloacae EN2 was resistant to all antibiotics (PAN), while Escherichia coli E11 recorded was the most sensitive bacteria to AgNPs and its resistant only to 3 antibiotics. unprogrammed use of antibiotics, resulting in bacterial strains resistant to manyantibiotics, or to all available antibiotics. Plants are a good source of primary andsecondary metabolites that have a major role in reducing silver nitrate to silvernanoparticles (AgNPs). The production of these nanoparticles were carried out by usingaqueous extract of Carthamus oxycantha M.Bieb. This can be verified by color changedof the reaction solution from yellow to dark brown because of the excitation of thesurface plasmon resonance. AgNPs were characterized by UV-Vis spectroscopy, whererecorded peak at 425 nm. Fourier Transformation-infrared (FTIR) was conducted toidentify the effective plant group that contributes to the formation of AgNPS and it wasfound that proteins and phenols have the major role in the formation of thosenanoparticles. Shapes and sizes of synthesized AgNPs were characterized by ScanningElectron Microscope (SEM) with a range of 50-80nm in size and spherical in shapes.Antibacterial activity of AgNPs were tested against Multi-Drug Resistant bacteria(MDR), Extremely antibiotics Resistant (XDR), and Pandrug-resistant (PAN) bacteria,was done in concentrations ranging from 1000-63 µg/ml. The result showed that theconcentrations from 1000-125 µg/ml inhibited all tested bacterial strains except the S1strain
The current study aimed to study the resistance of some species Bacterial resistance to the antibiotic vancomycin. 121 samples were collected and grown on Chocolate Agar medium for isolating pathogenic bacteria. and then It was grown on Nutrient Agar medium for pure colonies. These bacteria were diagnosed using phenotypic methods, biochemical tests, and 16SrRNA gene. The results reported that 40 (33.3%) resistant strains appeared, of which 13 were males with a percentage of (32.5%), while the number of female samples was 27 with a percentage (67.5%) and the rest were sensitive to this antibiotic. done during the study The current study obtained four types of bacteria resistant to vancomycin: 24 strains (60%) belonging to Pseudomonas aeruginosa bacteria, 11 strains (27.5%) belonging to Proteus mirabilis bacteria, 4 strains (10%) of Staphylococcus aureus bacteria, and one strain (2.5%) for Actinomyces sp. As for the presence of resistance genes, Vancomycin, where the current study recorded the presence of 17 bacterial strains containing both VanA and VanB genes (42.5%), while the VanA gene was present in 8 strains (20%), and the VanB gene was present in 6 strains (15%), and 9 appeared Strains (22.5%) do not possess either of the two genes (VanA and VanB), although they were resistant to vancomycin, and this may be due to their possession of other resistance genes or other mechanisms of resistance to this antibiotic.
The goal of this research is to use dental caries patients’ oral cavity-isolated Enterobacter aerogenes bacterial strains (S1, S2 and S3) to create silver nanoparticles (AgNPs) in an environmentally friendly and cost-effective manner. In addition, the study explores the combination of antibiotics with Streptococcus mitis MDR, which was isolated from patients with dental caries to determine their antibacterial efficiency. Clinical bacterial strains identified from dental caries patients' mouths were all resistant to standard antibiotics. Antibiotics and AgNPs have a synergistic impact, which suggests that antibiotics will make up a larger portion of the diet. It was shown that erythromycin E had the greatest synergistic impact with AgNPs (0.1 mg/ml), but Streptomycin and Tetracycline had only 6 mm inhibitory zones when paired with AgNPs (0.1 mg/ml) in comparison. Antagonizing effects are meant by this. It was revealed that antibiotics such as penicillin P and cephalexin CN had distinct effects on patients. When used in combination with antibiotics, Enterobacter aerogenes AgNPs demonstrated excellent antibacterial efficacy on Streptococcus mitis isolates. As a result, AgNPs in the dental care area have a wide range of applications.
In the present study , Madagascar Periwinkle Catharanthus roseus (L.)G.Don alkaloids were evaluated against two cancer cell lines :larynx human cancer cell line Hep-2,and mouse breast cancer cell line AMN3 , by using four concentrations for each cell line (25,12.5,6,25,3.125)mg/ml(in triplicate).and it has been found that alkaloids showed highest activity against larynx human cancer cell line Hep-2 ,while exhibited weak activity against mouse breast cancer cell line AMN3 compared with control treatment.
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