Occurrence of Ureide Accumulation in Soybean Plants

Yoneyama, T.; Karasuyama, Mitsuaki; Kouchi, Hiroshi; Ishizuka, Junji

doi:10.1080/17470765.1985.10555224

Cited by 19 publications

(6 citation statements)

References 18 publications

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“…The increased ureide and N-NH 4 + concentrations and unaffected N-NO 3 − concentrations suggest that BNF was enhanced by A. brasilense co-inoculation. Ureide and N-NH 4 + are the main N forms transported by the xylem after symbiotic (ureide) and associative BNF (N-NH 4 + ) [ 46 , 47 ]. Moreover, the isotopic technique analysis showed that the co-inoculation provided greater N accumulation in the shoot and grain specifically derived from soil and other sources (highlighting BNF), and had no effect on 15 N fertilizer recovery.…”

Section: Discussionmentioning

confidence: 99%

Co-Inoculation with Azospirillum brasilense and Bradyrhizobium sp. Enhances Nitrogen Uptake and Yield in Field-Grown Cowpea and Did Not Change N-Fertilizer Recovery

Galindo

Pagliari

Silva

et al. 2022

Plants

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This study was designed to investigate the effects of Azospirillum brasilense and Bradyrhizobium sp. co-inoculation coupled with N application on soil N levels and N in plants (total N, nitrate N-NO3− and ammonium N-NH4+), photosynthetic pigments, cowpea plant biomass and grain yield. An isotopic technique was employed to evaluate 15N fertilizer recovery and derivation. Field trials involved two inoculations—(i) single Bradyrhizobium sp. and (ii) Bradyrhizobium sp. + A. brasilense co-inoculation—and four N fertilizer rates (0, 20, 40 and 80 kg ha−1). The co-inoculation of Bradyrhizobium sp. + A. brasilense increased cowpea N uptake (an increase from 10 to 14%) and grain yield (an average increase of 8%) compared to the standard inoculation with Bradyrhizobium sp. specifically derived from soil and other sources without affecting 15N fertilizer recovery. There is no need for the supplementation of N via mineral fertilizers when A. brasilense co-inoculation is performed in a cowpea crop. However, even in the case of an NPK basal fertilization, applied N rates should remain below 20 kg N ha−1 when co-inoculation with Bradyrhizobium sp. and A. brasilense is performed.

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Section: Discussionmentioning

confidence: 99%

Co-Inoculation with Azospirillum brasilense and Bradyrhizobium sp. Enhances Nitrogen Uptake and Yield in Field-Grown Cowpea and Did Not Change N-Fertilizer Recovery

Galindo

Pagliari

Silva

et al. 2022

Plants

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“…Consequently, the caution first expressed in the literature regarding acetylene reduction assays has spilled over to other procedures including 15N techniques and the xylem solute analysesS.J6-zL In this context ureide formation by legumes in response to uptake of combined nitrogen in the form of ammonium (see ref. 6) is potentially very significant for measurements of symbiotic activity by xylem solute analyses. This specifically applies under conditions facilitating ammonium uptake by plants, since most calibrations of the method are based on nitrate as the sole source of mineral nitrogen.…”

Section: Discussionmentioning

confidence: 99%

Does ammonium uptake influence xylem sap composition inPhaseolus vulgaris L. andGlycine max (L.) Merill?

1995

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Abstract. The question of whether ammonium uptake influences the occurrence of ureides in legumes has been addressed in this study by investigating three P. vulgaris genotypes as well as one cultivar of Glycine max. All plants were raised in sand culture during the dry season in northern Thailand and irrigated daily with nitrogen-free nutrient solution, or the same solution containing 12 mol m -3 nitrogen in the form of (NH4)2SO 4 or KNO3, each treatment consisting of different proportions of either compound. Regression analyses of xylem sap composition relative to ammonium vs. nitrate supply of plants harvested at V4, R1 and R6 indicated close positive correlations of xylem amino nitrogen content and negative correlations with xylem nitrate content and ammonium supply. Statistically significant correlations between relative xylem ureide content and ammonium availability could be established for the P. vulgaris cultivar Brilliant up to stage R1, but not for the other plants investigated. It was concluded that at least for some genotypes of common bean a relationship exists between ureide production and ammonium uptake by the root system. Since the extent to which ureide production is stimulated remains quite small, its relevance to the xylem solute technique for measurement of N2 fixation may be limited. Nevertheless, due to the possibility of large genotypic differences in the impact of ammonium on ureide production, this factor must be considered in calculations if N2 fixation is to be determined in soils containing significant amounts of ammonium, e.g. in paddy fields. Key words. Allantoic acid; allantoin; ammonium; nitrate; common bean; soybean; xylem ureide assay.Quantification of N2 fixation under field conditions remains an important tool for legume research, ultimately aiming to enhance symbiotic activity of legume crops. The choice of method depends largely on the specific objective of the experiment, the conditions and species to be evaluated, and the analytical means available to the researcher. While 15N techniques are direct measures of symbiotic activity and may have certain advantages, indirect assessment procedures are considerably cheaper and can be conducted by laboratories with a less sophisticated technical set-up. Apart from the controversial acetylene reduction assay, the xylem solute technique is a potentially reliable indirect method to quantify symbiotic activity in the field. However, such indirect procedures are only as reliable as the calibration used to convert collected data to estimates of N2 fixation. Conventional calibrations rest upon assessments of changes in xylem sap composition in a series of plants treated with different concentrations of 15N-labelled nitrate. Since such long-term nitrate applications at various concentrations induce plants to develop changes in xylem sap composition. The method has proven most reliable in ureide (e.g. allantoin and allantoic acid)-producing plants (e.g. soybean), consistently reflecting a positive correlation between N2 fixation and the r...

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“…In both nitrate-and urea-fed plants, the internal nitrogen status, reflected by leaf greenness, may be enough to maintain photosynthetic ability. However, the chlorotic and/or necrotic lesions of the upper leaves of ammonium-fed plants increased in severity with increasing nutrient solution concentrations, likely because of ammonium toxicity (Tadano & Tanaka 1976;Ikeda & Osawa 1979;Yoneyama et al 1985) rather than nitrogen deficiency. Although Xia et al (2017) reported that the growth of soybean seedlings in a sand substrate was promoted by irrigation with a nutrient solution containing up to 100 ppm NH4-N (7.14 mM), our results suggest that silica sand is nearly sterile, with minimal nitrifying bacteria that convert ammonium to nitrate, and the excess of ammonium remaining in the rhizosphere prior to absorption might result in ammonium toxicity, even when plants are subirrigated with a 25% treatment solution (4.33 mM NH4-N).…”

Section: Optimal Form and Concentration Of Nitrogenmentioning

confidence: 99%

“…According to Yoneyama et al (1985) and Ohyama et al (2013), the inhibitory effect of nitrate is stronger than that of ammonium or urea for the nodulation and nitrogen fixation activity of soybean plants. However, to our surprise, ammonium-fed plants never formed root nodules irrespective of the ammonium concentration (Fig.…”

Section: Optimal Form and Concentration Of Nitrogenmentioning

confidence: 99%

“…Regarding nodulation, several reports indicated that urea-fed plants formed more and larger root nodules than nitrate-fed plants (Yoneyama et al 1985;Paradiso et al 2014bParadiso et al , 2015, but these observations were following treatments with high nitrogen concentrations (7.5-20 mM), which are highly inhibitory to nodulation, especially when supplied as nitrate. Therefore, urea can be used as the nitrogen source for promoting the early growth of inoculated soybean plants, but may not be necessarily superior to nitrate for nodulation at relatively low nitrogen concentrations.…”

Section: Optimal Form and Concentration Of Nitrogenmentioning

confidence: 99%

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Production of Soybean Plants for Hydroponic Cultivation from Seedling Cuttings in a Medium Containing Rhizobium Inoculum Depending on Various Concentrations of Nutrient Solution and Different Nitrogen Sources

Hata

Futamura

2020

Journal of Horticultural Research

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There has been an increasing interest in soybean cultivation in hydroponic systems. Because soybean plants can utilize nitrogen derived from biological N2 fixation, the use of Rhizobium inoculated plants may eliminate or decrease the need for mineral nitrogen fertilization in hydroponic systems. Thus, the objective of this study was to establish effective methods for making inoculated soybean transplants for a substrate-based hydroponic system. Inoculated plants were effectively produced by rooting seedling stem cuttings in a medium containing a Rhizobium inoculant. We also investigated the effects of different nitrogen forms and sub-irrigated nutrient solution concentrations on the growth and root nodule formation of the inoculated plants. The application of a nitrogen-free nutrient solution had minimal positive effects on the growth and nodulation of the inoculated plants. Ammonium-fed plants lacked root nodules regardless of the ammonium concentration. Furthermore, a 50% nutrient solution combining with nitrate or urea as the nitrogen source did not promote root nodulation. Therefore, inoculated plants should be subirrigated with an approximately 25% nutrient solution supplemented with nitrate or urea to induce early growth and nodulation prior to transplantation.

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Occurrence of Ureide Accumulation in Soybean Plants

Cited by 19 publications

References 18 publications

Co-Inoculation with Azospirillum brasilense and Bradyrhizobium sp. Enhances Nitrogen Uptake and Yield in Field-Grown Cowpea and Did Not Change N-Fertilizer Recovery

Co-Inoculation with Azospirillum brasilense and Bradyrhizobium sp. Enhances Nitrogen Uptake and Yield in Field-Grown Cowpea and Did Not Change N-Fertilizer Recovery

Does ammonium uptake influence xylem sap composition inPhaseolus vulgaris L. andGlycine max (L.) Merill?

Production of Soybean Plants for Hydroponic Cultivation from Seedling Cuttings in a Medium Containing Rhizobium Inoculum Depending on Various Concentrations of Nutrient Solution and Different Nitrogen Sources

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