Occurrence of tomato leaf curl Bangladesh virus and associated subviral DNA molecules in papaya in Bangladesh: molecular detection and characterization

Hamim, Islam; Borth, Wayne B.; Melzer, Michael; Suzuki, Jon Y.; Wall, Marisa M.; Hu, John

doi:10.1007/s00705-019-04235-8

Cited by 8 publications

(4 citation statements)

References 25 publications

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“…Monopartite begomoviruses are usually found to be associated with DNA satellites. This association may change the host range or increase the pathogenicity of the virus [6].…”

Section: Introductionmentioning

confidence: 99%

Transreplication Preference of the Tomato Leaf Curl Joydebpur Virus for a Noncognate Betasatellite through Iteron Resemblance on Nicotiana bethamiana

Vo,

Wira Sanjaya,

Kil

et al. 2023

Microorganisms

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Pepper plants (Capsicum annuum) with severe leaf curl symptoms were collected in 2013 from Bangalore, Karnataka, India. The detection results showed a co-infection between the tomato leaf curl Joydebpur virus (ToLCJoV) and tomato leaf curl Bangladesh betasatellite (ToLCBDB) through the sequencing analysis of PCR amplicons. To pinpoint the molecular mechanism of this uncommon combination, infectious clones of ToLCJoV and two different betasatellites—ToLCBDB and tomato leaf curl Joydebpur betasatellite (ToLCJoB)—were constructed and tested for their infectivity in Nicotiana benthamiana. Together, we conducted various combined agroinoculation studies to compare the interaction of ToLCJoV with non-cognate and cognate betasatellites. The natural non-cognate interaction between ToLCJoV and ToLCBDB showed severe symptoms compared to the mild symptoms of a cognate combination (ToLCJoV × ToLCJoB) in infected plants. A sequence comparison among betasatellites and their helper virus wasperformed and the iteron resemblances in ToLCBDB as well as ToLCJoB clones were processed. Mutant betasatellites that comprised iteron modifications revealed that changes in iteron sequences could disturb the transreplication process between betasatellites and their helper virus. Our study might provide an important consideration for determining the efficiency of transreplication activity between betasatellites and their helper virus.

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“…Monopartite begomoviruses are usually found to be associated with DNA satellites. This association may change the host range or increase the pathogenicity of the virus [6].…”

Section: Introductionmentioning

confidence: 99%

Transreplication Preference of the Tomato Leaf Curl Joydebpur Virus for a Noncognate Betasatellite through Iteron Resemblance on Nicotiana bethamiana

Vo,

Wira Sanjaya,

Kil

et al. 2023

Microorganisms

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“…In India, PRSV is one of the greatest concerns, with the potential to cause 100 percent yield loss [7], followed by PaLCuV. A survey of the literature showed that the incidence of papaya leaf curl disease is greater in Asian countries (Bangladesh, China, India, Iran, Korea, Nepal, Oman, Pakistan, and Taiwan) [8][9][10][11][12][13][14][15][16]. Recently, the virus is also reported in the USA and Mexico, with disease incidence ranging from 40 to 100 percent [6,17].…”

Section: Introductionmentioning

confidence: 99%

Identification of Novel Begomoviruses Associated with Leaf Curl Disease of Papaya (Carica papaya L.) in India

et al. 2022

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Papaya (Carica papaya L.) is one of the most important fruit crops grown in tropical and subtropical regions of the world. Papaya leaf curl disease is one of the greatest concerns next to Papaya ring spot disease for India and the world. A survey was conducted during the year 2019 to 2021 for assessing the leaf curl disease incidence in five major papaya-growing districts of Karnataka State, India. The incidence ranged from 10 to 21 percent, with plants expressing typical begomovirus symptoms. Thirty-two virus-infected papaya samples (PLC-1 to PLC-32), collected from different farmer’s fields, gave positive amplification for begomovirus detection. Based on the partial genome analysis, 13 representative papaya leaf curl isolates were selected for complete genome amplification by rolling circle DNA amplification (RCA). The RCA products were cloned, sequenced and analyzed. Based on the analysis and strain classification criteria for begomoviruses, five isolates (PLC-2, 3, 9, 11 and 18) were considered variants of Chilli leaf curl virus (ChiLCV). Isolate PLC-22 is considered a strain of ChiLCV, with 93.5% nt identity sharing. Similarly, isolate PLC-28 is considered a strain of Croton yellow vine mosaic virus (CYVMV), and isolates PLC-25 and PLC-31 were considered as strains of Papaya leaf curl virus (PaLCuV). Among the remaining four isolates, three (PLC-1, PLC-4 and PLC-7) share more than 91% nt identity among them and less than 91% nt identity with all other reported begomovirus isolates. Hence, they are considered to be isolates of the novel begomovirus, and the name Papaya leaf curl Bagalkote virus [India:Karnataka:Bagalkote:Papaya:2021] is proposed. One isolate (PLC-32) is also found to be distinct from all other begomovirus isolates, including the isolates in the current study also considered to be novel begomovirus, for which we propose the name Papaya leaf curl Haveri virus [India:Karnataka:Haveri:Papaya:2021]. The putative recombination analysis of all 13 papaya isolates showed that a major part of the viral genome was likely descended from the begomoviruses reported previously. This is the first report on the diversity and a distribution of the begomoviruses infecting papaya in Karnataka, India. The current investigation results revealed five major papaya-infecting begomoviruses (PaLCuBKV, ChiLCV, PaLCuV, CYVMV and PaLCuHV) in the sampled regions.

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“…Due to its dietary benefits and demand, papaya fruit production has significantly increased over the last few decades in different geographical countries. Meanwhile, different BGVs such as okra enation leaf curl virus (OELCuV), cotton leaf curl Gezira virus (CLCuGeV), papaya leaf curl virus (PaLCV), papaya leaf curl China virus (PaLCCNV), ageratum yellow vein virus (AYVV), tomato leaf curl Bangladesh virus (ToLCBaV), and radish leaf curl virus (RaLCV) have been found to infect this fruit plant in different countries(Chang et al 2003;Shahid et al 2013;Shen et al 2014;Bananej et al 2016;Tang et al 2018;Hamim et al 2019;Nehra et al 2019;Bananej et al 2021). Although a cotton infecting monopartite BGV (CLCuGeV) has been identified which infects papaya plants in Oman(Khan et al 2012), none of the bipartite BGV has been reported to infect papaya plants in the country.…”

mentioning

confidence: 99%

Journal of Plant Protection Research

Shahid

Al-Sadi

2022

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The complete genome sequence of tomato leaf curl Palampur virus (ToLCPlV), that infects a papaya (Carica papaya) plant, was determined. The virus genome was composed of 2,756 and 2,719 nucleotides (nt) in length, encoding all proteins required for replication, encapsidation and movement with the genome features typical of a bipartite begomovirus. Pairwise identity, derived using the Sequence Demarcation Tool (SDT), identified that the virus DNA A and DNA B shared maximum sequence identity 98-99% corresponding to the DNA A of ToLCPlV ([IR: Jir-T65X:08] JF501720) and 96-98% to the cognate partner DNA B of ToLCPlV ([IR: Jir1:T55P:07] FJ660423), respectively. The evolutionary relation using phylogenetic dendrograms of DNA A and DNA B genome components were clustered with ToLCPlV genomes of DNA A and DNA B of Iranian isolates. This study provides the first evidence of a bipartite ToLCPlV infecting papaya in the Sultanate of Oman and also indicates the requirement for more surveillance of this virus in Oman, as ToLCPlV is a major threat to tomato and other vegetable crops in South Asia (India and Pakistan) and in Iran.

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Occurrence of tomato leaf curl Bangladesh virus and associated subviral DNA molecules in papaya in Bangladesh: molecular detection and characterization

Cited by 8 publications

References 25 publications

Transreplication Preference of the Tomato Leaf Curl Joydebpur Virus for a Noncognate Betasatellite through Iteron Resemblance on Nicotiana bethamiana

Transreplication Preference of the Tomato Leaf Curl Joydebpur Virus for a Noncognate Betasatellite through Iteron Resemblance on Nicotiana bethamiana

Identification of Novel Begomoviruses Associated with Leaf Curl Disease of Papaya (Carica papaya L.) in India

Journal of Plant Protection Research

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