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Fruit rot (Phomopsis vexans) of brinjal is an important disease of Northern dry zone of Karnataka resulting into heavy losses. Recently the production of brinjal has been drastically reduced due to incidence of fruit rot disease caused by a fungus Phomopsis vexans. An investigation was carried out to test the efficacy of fungicides, botanicals and bio-agents in vitro. Among fungicides tested carbendazim, tebuconazole and hexaconazole at 0.25, 0.5, 0.75 and 1.00 per cent concentration showed high inhibition of 100 per cent of mycelial growth of the pathogen. The least mycelial inhibition was observed in case of captaf (63.33%).The results of botanicles tested in vitro revealed that highest inhibition of mycelial growth of Phomopsis vexans was observed in 5 and 10 per cent of garlic extract, kokum extract and onion extract. The results of dual culture technique revealed that fungal bio agents were better than bacterial bioagents in inhibiting the growth of fruit rot pathogen. Phomopsis vexans was effectively inhibited by T. harzianum-p.