Occurrence of Salmonella genomic island 1 (SGI1) in two African Proteus mirabilis strains isolated from diseased chicken flocks

Soliman, Ahmed M.; Saad, Alaaeldin Mohamed; Ahmed, Ashraf M.; Al-baqir, Ahmed; Hussein, Hussein Awad; Nariya, Hirofumi; Shimamoto, Tadashi

doi:10.1016/j.meegid.2018.04.008

Cited by 7 publications

(5 citation statements)

References 11 publications

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“…Recently, we reported the detection of SGI1-PmABB in the P. mirabilis strain PmTAN59 in the same hospital (6). In addition, we reported the detection of clonally related SGI1-W-carrying P. mirabilis strains isolated from chickens and patients in Egypt (9). These findings indicated that SGI1-W might be distributed in the Egyptian sector.…”

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“…To detect the presence of any deletion or insertion in the SGI1-W backbone, primer sets demonstrating almost all SGI1 backbone genes were used in the PCR amplification, as previously described (see Table S1) (2,6,9). The results indicated that PsTAN3 carried the entire SGI1 sequence.…”

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confidence: 99%

“…The results indicated that PsTAN3 carried the entire SGI1 sequence. Additionally, all the SGI1-W-positive strains reported globally were characterized by the absence of any deletion or insertion in the SGI1-W backbone genes (6,7,9). The absence of any deletion in ORF S005 (traN) in SGI1-W of PsTAN3 and the presence of two intact ORFs (S006 and S007) coding for homologs of FlhDC SGI1 will result in increasing SGI1 maintenance, transfer, and/or spreading.…”

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Emergence of Salmonella Genomic Island 1 Variant SGI1-W in a Clinical Isolate of Providencia stuartii from Egypt

Soliman

Nariya

Shimamoto

2019

Antimicrob Agents Chemother

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M ost of the variants of Salmonella genomic island 1 (SGI1) are multidrug resistance (MDR) DNA elements that can be transferred by horizontal gene transfer (HGT) (1). SGI1 was initially described in Salmonella enterica serovar Typhimurium DT104 (1). It consists of a 27.4-kb backbone (28 open reading frames [ORFs]) with an MDR region consisting of a 15-kb complex class 1 integron of the family In104 (1). The SGI1 variant SGI1-L was detected for the first time in Proteus mirabilis and Morganella morganii strains from Palestine in 2006 and from France in 2017, respectively (2, 3). The 3= end of the chromosomal trmE gene is the SGI1-specific integration site in the SGI1-carrying strains (3, 4). Providencia stuartii is Gram-negative bacterium of the family Morganellaceae commonly associated with urinary tract infections or indwelling urinary catheters in hospitalized and nursing home patients (5). It has intrinsic resistance to polymyxin, tigecycline, aminopenicillin, and first-generation cephalosporins (5). Here, we report the first identification (to our knowledge) of SGI1 in a multidrugresistant clinical isolate of P. stuartii. The strain, designed PsTAN3, was isolated from a wound pus swab from a male patient in a teaching hospital in Tanta City, Egypt, in 2014. PsTAN3 was resistant to aztreonam (MIC, Ն512 g/ml), ciprofloxacin (MIC, Ն16 g/ml), chloramphenicol (MIC, Ն512 g/ml), colistin (MIC, Ն128 g/ml), gentamicin (MIC, Ն128 g/ml), tetracycline (MIC, Ն128 g/ml), streptomycin (MIC, Ն512 g/ml), cefoxitin (MIC, Ն256 g/ml), cefoperazone (MIC, Ն512 g/ml), trimethoprim (MIC, Ն512 g/ml), cefotaxime (MIC, Ն512 g/ml), meropenem (MIC, Ն16 g/ml), and nalidixic acid (MIC, Ն512 g/ml) but sensitive to doripenem (MIC, Յ1 g/ml). PsTAN3 was negative by the modified carbapenem inactivation method (mCIM), indicating the absence of carbapenemase production. PCR and DNA sequencing were used to map the entire SGI1 (for a list of PCR primers, see Table S1 in the supplemental material) and identified variant SGI1-W (6, 7). The multidrug resistance region was 6.54 kb with the genetic arrangement intI1-aadA2-lnuF-qacE⌬1-sul1-orf5-orf6-IS6100 (PsTAN3 class 1 integron GenBank accession no. LC370342) (Fig. 1). SGI1-W was integrated at the 3= end of the trmE gene (Fig. 1). The strain also carried bla TEM-1 , bla DHA-1 , qnrD, and floR genes conferring resistance to third-generation cephalosporins, ciprofloxacin, florfenicol, and chloramphenicol, respectively, and the class 1 integrons aadA2 and lnuF. The MDR region of SGI1 in PsTAN3 was 100% identical to that of SGI1-PmC105 reported in a P. mirabilis isolate from a poultry farm in China (7). Furthermore, the circular form was detected in PsTAN3 after two steps of DNA amplification using primers SGI1circ1 and SGI1circ2 with the expected target size (364 bp) (GenBank accession no. LC370343) (see Fig. S1A in the supplemental material). The sequence of the SGI1-W attP site in PsTAN3 (5=-TTCTGTATCGGGAAGTAA-3=) differs in 1 bp (C¡T) from that of S. enterica DT104 and P. mirabilis (5=-TTCTGTATTGGGAAG...

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Emergence of Salmonella Genomic Island 1 Variant SGI1-W in a Clinical Isolate of Providencia stuartii from Egypt

Soliman

Nariya

Shimamoto

2019

Antimicrob Agents Chemother

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“…Proteus mirabilis has been recognized as one of the most common pathogens associated with nosocomial infections ( Armbruster et al, 2017 ), particularly of the urinary and respiratory tracts, traumatic wounds, and surgical sites. Salmonella genomic island 1 (SGI1), a site-specific integrative mobilizable element initially found in Salmonella enterica serovar Typhimurium DT104 ( Boyd et al, 2000 ), has emerged recently in P. mirabilis strains from diverse sources in China ( Lei et al, 2014 , 2015 ; Qin et al, 2015 ), France ( Schultz et al, 2015 ; de Curraize et al, 2020 ), Egypt ( Soliman et al, 2017 , 2018 ) and South Korea ( Sung et al, 2017 ). Salmonella genomic island 1 and its variants often carry numerous antimicrobial resistance genes and facilitate the emergence of multidrug resistant (MDR) P. mirabilis , thus posing a significant threat to public health.…”

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Nosocomial Outbreak of Carbapenemase-Producing Proteus mirabilis With Two Novel Salmonella Genomic Island 1 Variants Carrying Different blaNDM–1 Gene Copies in China

Yang¹,

Chen

et al. 2022

Front. Microbiol.

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NDM-1-producing multidrug-resistant Proteus mirabilis brings formidable clinical challenges. We report a nosocomial outbreak of carbapenem-resistant P. mirabilis in China. Six P. mirabilis strains collected in the same ward showed close phylogenetic relatedness, indicating clonal expansion. Illumina and MinION sequencing revealed that three isolates harbored a novel Salmonella genomic island 1 carrying a blaNDM–1 gene (SGI1-1NDM), while three other isolates showed elevated carbapenem resistance and carried a similar SGI1 but with two blaNDM–1 gene copies (SGI1-2NDM). Four new single nucleotide mutations were present in the genomes of the two-blaNDM–1-harboring isolates, indicating later emergence of the SGI1-2NDM structure. Passage experiments indicated that both SGI variants were stably persistent in this clone without blaNDM–1 copy number changes. This study characterizes two novel blaNDM–1-harboring SGI1 variants in P. mirabilis and provides a new insight into resistance gene copy number variation in bacteria.

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“…Some SGI1 variants were detected that carried resistance genes to frontline and clinically significant antibiotics, e.g., bla NDM-1 (carbapenem resistance) (17), bla VEB-6 (8), bla CTX-M-15 (cephalosporin resistance) (18), and rmtC (aminoglycoside resistance) ( 19), posing a serious public health threat. Besides detection of SGI1-C-Kp, we reported in Egypt (i) an SGI1-W variant in a P. mirabilis strain from the same hospital (3), (ii) clonally related SGI1-W-carrying P. mirabilis strains isolated from chickens and patient (20), and (iii) the first SGI1-W-positive P. stuartii isolate (4). These findings indicated that SGI1 variants might be horizontally circulated in Egypt among Enterobacterales and may be transferred to other Gram-negative bacterial species.…”

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Emergence of Salmonella Genomic Island 1 Variant SGI1-C in a Multidrug-Resistant Clinical Isolate of Klebsiella pneumoniae ST485 from Egypt

Soliman

Ramadan

et al. 2020

Antimicrob Agents Chemother

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Occurrence of Salmonella genomic island 1 (SGI1) in two African Proteus mirabilis strains isolated from diseased chicken flocks

Cited by 7 publications

References 11 publications

Emergence of Salmonella Genomic Island 1 Variant SGI1-W in a Clinical Isolate of Providencia stuartii from Egypt

Emergence of Salmonella Genomic Island 1 Variant SGI1-W in a Clinical Isolate of Providencia stuartii from Egypt

Nosocomial Outbreak of Carbapenemase-Producing Proteus mirabilis With Two Novel Salmonella Genomic Island 1 Variants Carrying Different blaNDM–1 Gene Copies in China

Emergence of Salmonella Genomic Island 1 Variant SGI1-C in a Multidrug-Resistant Clinical Isolate of Klebsiella pneumoniae ST485 from Egypt

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