Occurrence of S and F1C/S-related fimbrial determinants and their expression inEscherichia colistrains isolated from extraintestinal infections: Table 1

Abstract

The presence of S and F1C/S-related fimbrial determinants was determined in 462 E. coli strains obtained from different extraintestinal infections and in 162 control isolates of E. coli by using two different DNA probes: an oligonucleotide probe consisting of three oligonucleotides that bind specifically to the S adhesin gene and a polynucleotide probe which is not able to distinguish between S, F1C, and S-related sequences. The expression of S and F1C phenotypes was tested by dot enzyme immunoassay with the corresponding monoclonal antibodies. S fimbriae genotypes were observed more frequently in septic (25%) and urinary (12%) isolates of E. coli than in faecal and water isolates (1%) and often occurred together with O2, O6, O18 and O83 antigens. F1C/S-related fimbrial DNA was detected with a higher frequency in UTI isolates (26%) than in septic (16%) and faecal (10%) isolates and was most frequently associated with O4, O6, and O75 serotypes. Since the production of S and F1C fimbriae was comparatively rare in all clinical and control isolates of E. coli, DNA hybridization assays which allow the sensitive and specific detection of fimbrial determinants even in the absence of their expression are preferable to phenotypic assays.

Escherichia colimediated urinary tract infections: Are there distinct uropathogenicE. coli(UPEC) pathotypes?

“…S fimbriae of E. coli bind to sialyl galactosides and are implicated in experimental UTI in rats [44]. UTI isolates are at least two times more likely to carry S fimbriae genes than fecal isolates [13,35]. More recently, two additional potential adherence factors, encoded by the genes hra and iha, were associated with UPEC isolates.…”