Occurrence of Pasteurellosis and Newcastle Disease in indigenous chickens in Sirajgonj district

Abstract

A study was carried out on indigenous layer chickens to know occurrence of Avian Pasteurellosis (AP) and Newcastle disease (ND) in Sirajgonj during the period from January/2012 to December/2013. The clinical signs showed before death was recorded by taking history and the birds were subjected to post mortem examination. In addition to the clinical and necropsy findings, ND was detected by Anigen® rapid antigen detection kit. The AP was confirmed by isolation and identification of Pasteurella (P.) multocida from liver, spleen and heart samples. The P. multocida was found to grow on nutrient broth, nutrient agar, blood agar and Eosin Methylene Blue agar where it produced whitish, opaque, round, flat, translucent colonies. It produced turbidity on nutrient broth. The organism was not found to grow on MacConkey agar media and did not cause hemolysis on blood agar media. The impression smear of liver and heart blood were stained by Gram's staining, Leishman staining and Methylene blue staining techniques to detect P. multocida. P. multocida organism was also found to fermnt dextrose, lactose and mannitol with the production of only acid but did not ferment maltose and lactose. P. mutocida was found non-motile, indole positive and urease negative. On triple sugar iron test it produced H2S and fermented only glucose. It was found negative to both methyl red test and Voges Proskauer test. Out of 360 birds tested 59.72% (215) was AP positive and 40.28% was ND positive. Age, Sex, season and mortality due to other diseases were not considered.

Molecular Studies on Pasteurella Species Isolated From Ducks

“…Leishman's staining technique revealed bipolar microrganisms. These features were in agreement with previous researches (Akhtar, 2013;Belal, 2013 andIevy et al, 2013).…”

Phenotypic, Genotypic, Multidrug Resistance Genes and Disinfectant Biocidal Effect of Pasteurella Multocida Isolated From Chickens

“…The prevalence of P.multocida (4 %; n=12/300) in this study was lower than the value reported in the earlier studies Hassan et al (2010) reported 12.05% prevalence in layer chicken and 4.25% in broiler chicken, whereas Hossain et al (2013) found 13.04% prevalence in chicken and Belal (2013) found 59.72% in backyard poultry. All the isolates subjected to mice inoculation tests killed the mice in 24 -48h.…”

Prevalence and Antimicrobial Susceptibility Profiles of <i>Pasteurella multocida</i> in Village Chickens (<i>Gallus gallus domesticus</i>) in Maiduguri, Borno State, Nigeria

“…The overall prevalence rate of 21.7% recorded in this research is considered apparently high among scavenging physically healthy village chicken flocks in the study area. This prevalence rate was found to be higher than 13.04% and 11.42% reported by Hossain et al [35] and Panna et al [53] respectively but however lower than 59.72% reported by Belal [10] in backyard poultry. The variation in the prevalence rate of P. multocida reported from various study might be associated to the number of samples tested, method employed in isolation, differences in age and breeds of the chickens as well as difference in management and husbandry system of chickens sampled.…”