Occurrence of natural triploidy in Rhamdia quelen (Siluriformes, Heptapteridae)

Tsuda, J.R.; Moraes, Vivian Patrícia Oliveira de; Giuliano-Caetano, Lúcia; Dias, Ana Lúcia

doi:10.4238/vol9-3gmr949

Cited by 13 publications

(7 citation statements)

References 19 publications

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“…(Pansonato‐Alves et al ); Gymnotus carapo (Fernandes‐Matioli et al ); Eigenmannia sp. (Almeida Toledo et al ); Hoplias malabaricus (Utsunomia et al ); Hoplerythrinus unitaeniatus (Giulino‐Caetano and Bertollo ); Richomycterus davisi (Borin et al ); Rhamdia quelen (Tsuda et al ); Astyanax schubartii (Morelli et al ); Astyanax sp. (Fauaz et al ; Kantek et al ); and Astyanax scabripinnis (Maistro et al ; Machado et al ).…”

Section: Discussionmentioning

confidence: 99%

Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Adamov

Nascimento

Maciel

et al. 2016

J World Aquaculture Soc

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Triploidization is an interesting tool to produce sterile fish. In the yellowtail tetra, Astyanax altiparanae, this can be applied for aquaculture and surrogate technologies. In this study, we compared the efficacy of cold (2 C) or heat shock (38 C, 40 C, and 42 C) on triploid induction in the yellowtail tetra. The eggs were treated with cold or heat shock, 2 min postfertilization (30 min in cold shock or 2 min in heat shock). Intact embryos served as the control group. Ploidy status was confirmed by karyotyping, flow cytometry, and nuclear diameter of erythrocytes. The hatching rate decreased after cold shock (12.69 ± 15.76%) and heat shock at 42 C (0.35 ± 0.69%) in comparison with the control group (63.19 ± 16.82%). At 38 C and 40 C, hatching rates (61.29 ± 17.73% and 61.75 ± 22.1%, respectively) were not decreased. Only one triploid arose at 38 C (1/80). At 40 C, a high number of triploids arose (72/78). At 42 C, very few embryos developed into the hatching stage. A large number of haploid individuals arose after cold shock (61/75), with only one triploid. Our results indicate that heat shocking of embryos at 40 C is optimum for triploid production in the yellowtail tetra.

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Section: Discussionmentioning

confidence: 99%

Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Adamov

Nascimento

Maciel

et al. 2016

J World Aquaculture Soc

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“…In natural populations of Carassius auratus for example, diploid, triploid and polyploid individuals coexist (Bai, Liu, Li & Yue ). Naturally occurring of triploids has been detected in Rhamdia quelen (Tsuda, De‐Moraes, Giuliano‐Caetano & Dias ). In the hybridization of female grass carp ( Ctenopharyngodon idellus ) with male blunt‐snout bream ( Megalobrama amblycephala ), diploid and triploid hybrids are generated (He, Xie, Li, Liu, Xiao, Hu, Wang, Qin & Liu ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of triploid hybrid groupers from interspecies hybridization (Epinephelus coioides♀ ×Epinephelus lanceolatus♂)

et al. 2014

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Interspecies hybridization is widely used in aquaculture as a beneficial strategy. Diploid and triploid hybrids have been detected from the interspecies hybridization of Epinephelus coioides ♀ 9 Epinephelus lanceolatus ♂. This is the first report of triploidization through hybridization in grouper. Confirmation has been obtained through flow cytometry, karyotyping and erythrocyte nuclei measurement. The chromosome numbers of E. coioides, E. lanceolatus, diploid hybrid grouper are 48 and triploid hybrid grouper are 72. Measurements of erythrocyte nuclei indicate that triploid fish have a larger nuclear surface than the diploid groupers, and the average ratio of triploid to diploid surface area is 1.59. During the first 1.5 years, triploid hybrid groupers grow faster than diploid hybrid groupers or either parent species. The average growth rate of triploid hybrids is 1.61 times greater than that of diploid hybrids at 6 months of age and 1.43 times greater at 18 months of age. The triploid hybrid groupers are inferior in gonadal development, with no primary-growth-stage oocytes appearing in the gonads at 18 months of age.Morphological studies indicate that triploid hybrid groupers have distinctive differences in snout length, eye diameter, body trunk shape, and tail shape development compared with diploid hybrid groupers. Triploid hybrid groupers have an advantage in growth ability, and artificial breeding of triploid groupers might be of great potential use in the grouper aquaculture industry.

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“…1d). The Taquari River and the Lindóia stream populations had been previously described by Moraes et al 30 and Tsuda et al, 31 respectively, by means of conventional staining, silver nitrate impregnation, and fluorochrome staining.…”

Section: Classic Cytogenetic Analysismentioning

confidence: 99%

Genetic and Chromosomal Differentiation ofRhamdia quelen(Siluriformes, Heptapteridae) Revealed by Repetitive Molecular Markers and DNA Barcoding

et al. 2019

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Rhamdia quelen, a species of Heptapteridae, is considered to be a complex because of taxonomic and phylogenetic inconsistencies. Determining the physical location of repetitive DNA sequences on the chromosomes and the DNA barcode might increase our understanding of these inconsistencies within different groups of fish. To this end, we analyzed R. quelen populations from two river basins in Brazil, Paraguay and Parana, using DNA barcoding and different chromosomal markers, including U2 snDNA, which has never been analyzed for any Rhamdia species. Cytochrome c oxidase I gene sequence analysis revealed a significant differentiation among populations from the Miranda and Quexada rivers, with genetic distances compatible to those found among different species in neotropical fishes. Our results, in general, revealed a conservative chromosomal evolution in R. quelen and a differential distribution of some markers, such as 5S rDNA and U2 snDNA, in different populations. We suggest that R. quelen must undergo a major revision in its morphological, genetic, and cytogenetic molecular and taxonomic structure to elucidate possible operational taxonomic units.

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Occurrence of natural triploidy in Rhamdia quelen (Siluriformes, Heptapteridae)

Cited by 13 publications

References 19 publications

Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Characterization of triploid hybrid groupers from interspecies hybridization (Epinephelus coioides♀ ×Epinephelus lanceolatus♂)

Genetic and Chromosomal Differentiation ofRhamdia quelen(Siluriformes, Heptapteridae) Revealed by Repetitive Molecular Markers and DNA Barcoding

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