Occurrence of monosialosyl pentahexaosylceramide GalNAc-GM1 as specific tumor-associated ganglioside of human head and neck squamous cell carcinomas

Bolot, G.; David, M. Jablons; Kasama, Takeshi; Taki, Takao; Handa, Shizuo; Richard, Michel; Pignat, J.-C.; Thomas, Luc; Portoukalian, Jacques

doi:10.1016/s0304-3835(98)00291-2

Cited by 7 publications

(12 citation statements)

References 15 publications

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“…Confronting GSL expression seen in our work with literature data, the only difference found was the presence of GD3, seen by Tatsumura et al (1988) and Bolot et al (1998) 20,22 . And, as we analyze GSL expression in head and neck SCC fragment collection obtained from six patients, Bolot et al (1999) found NAc-GM1 only in SCC, in minimal amounts 21 .…”

Section: Discussionmentioning

confidence: 95%

“…The only two articles, of the same group, that assessed GSL expression in carcinoma cells compared to head and neck normal tissue, leave doubts as to the histological type and the anatomic sites of the tumors studied; besides, the small number of tumors studied by this group, six cases only, do not correctly represent SCC GSL expression 21,22 . Another description of SCC GSL expression profile was carried out when they studied GSL expression during the differentiation process in SCC cells in the retromolar region 20 .…”

Section: Discussionmentioning

confidence: 99%

“…However, as far as head and neck SCC (squamous cell carcinoma), especially in upper airways and digestive tracts, there are few papers showing GSL expression in the SCC differentiation or malignant transformation, despite the fact that one of the first antibodies developed against GSL be able to identify SCC LacCer 1,15 . Only Bolot et al (1998Bolot et al ( , 1999 assessed GSL expression in head and neck carcinoma and normal tissue cells, notwithstanding these papers leave doubts in regards of histologic types and the anatomical sites of the tumors studied.…”

Section: Introductionmentioning

confidence: 99%

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Expressão de glicoesfingolipídeos no carcinoma espinocelular do trato aerodigestivo superior

Filho

Walder

Takahashi

et al. 2006

Rev. Bras. Otorrinolaringol.

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Os glicoesfingolipídios (GSLs) são importantes componentes da membrana celular, organizados em microdomínios, relacionados a receptores de membrana e comportamento anti-social da célula neoplásica como crescimento descontrolado, invasão e ocorrência de metástases. OBJETIVO: Como a expressão de GSLs no carcinoma espinocelular (CEC) é tema pouquíssimo estudado decidiu-se realizar estudo prospectivo visando avaliar a expressão de GSLs no CEC do trato aerodigestivo superior. MÉTODO: Coletou-se 33 amostras de CEC e mucosa normal e GSLs extraídos e purificados por cromatografia de fase reversa em coluna de C-18 e hidrólise alcalina em metanol. Os GSLs foram quantificados por densitometria das placas de cromatografia de alta resolução em camada delgada coradas com orcinol. RESULTADOS: Observou-se aumento significativo de GSLs no CEC (3,57µg/mg) em comparação à mucosa normal (1,92µg/mg), principalmente do monosialogangliosídeo (GM3), trihexosilceramida (CTH), dihexosilceramida (CDH), globosídeo (Gb4). A expressão de monohexosilceramida (CMH) foi semelhante no CEC e na mucosa normal. O aumento do GM3 no CEC foi demonstrado por métodos imunoquímicos empregando-se MAb DH2 (anti-GM3). Analisando-se os carboidratos do CMH por cromatografia gasosa acoplado a espectrômetro de massa constatou-se que a mucosa normal expressa glucosilceramida e o CEC glucosilceramida e galactosilceramida. CONCLUSÃO: O aumento de GSLs no tecido tumoral pode representar alterações dos microdomínios da membrana celular resultantes do processo de transformação maligna, responsáveis por uma maior interação célula-célula e célula-matriz aumentando seu potencial de infiltração e metástase, possibilitando o emprego dos GSLs e de MAbs no diagnóstico e no tratamento do CEC, a exemplo do que ocorre no melanoma.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expressão de glicoesfingolipídeos no carcinoma espinocelular do trato aerodigestivo superior

Filho

Walder

Takahashi

et al. 2006

Rev. Bras. Otorrinolaringol.

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“…Extensive studies have been performed to analyze the structure of GSLs from a variety of tumor tissues, including colon cancer (Siddiqui et al 1978 ), melanoma (Portoukalian et al 1979 ), gastrointestinal cancers (Magnani et al 1982 ), head and neck squamous cell carcinoma (Bolot et al 1999 ), metastatic brain tumors (Hamasaki et al 1999 ), and renal cell carcinoma (Ito et al 2001 ). Furthermore, a series of GSLs that abnormally accumulate in cancerous tissues have been successfully isolated and analyzed (Hakomori et al 1983(Hakomori et al , 1984Fukushi et al 1984a , b ) is observed in most human adenocarcinoma (Hakomori et al 1984 ;Fukushi et al 1984b ;Nudelman et al 1986a , b ), while GD3 is observed in melanoma (Portoukalian et al 1979 ).…”

Section: Gslsmentioning

confidence: 99%

Glycomic Analysis of Cancer

Miyamoto

2014

Sugar Chains

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Remarkable alterations in oligosaccharide structures are associated with many human diseases, including cancers. Numerous clinicopathological and biochemical studies have suggested the involvement of aberrant glycosylation in cancer malignancy, such as metastasis and invasion. Furthermore, altered carbohydrate determinants, including tumor-associated carbohydrate antigens such as SLe a (CA19-9), have been utilized as useful tumor markers for the diagnosis of cancer. Cancer glycomic analysis (i.e., precise and comprehensive analysis of altered oligosaccharides in cancer tissues and sera) is a widely used tool for (1) investigating the involvement of glycosylation in cancer malignancy and (2) discovering novel carbohydrate tumor marker candidates. Comprehensive clinico-glycomic studies of glycosphingolipids of colorectal cancers have revealed specifi c alterations related to malignant transformation, as well as characteristic alterations associated with clinical features. Glycomic analyses of colorectal cancers and pancreatic cancers revealed the presence of two kinds of novel fucogangliosides, sialylated type1H (Lewis-negative specifi c antigen) and sialylated type2H, both of which are isomers of sialyl Le x and sialyl Le a . The accumulation of free oligosaccharides in human cancers has been elucidated. Free Neu5Ac-containing complex-type N -glycans accumulated in pancreatic cancers. In addition to these free oligosaccharides, free KDN-containing complex-type N -glycans accumulated in prostate cancers. N -linked and O -linked glycans have also been targets for cancer glycomics. In particular, extensive studies of serum glycomic analyses have been performed to fi nd novel glycan cancer biomarker utilizing newly developed high-throughput platform technologies. It is anticipated that these cancer glycomic studies will lead to the discovery of glycan biomarker or therapy targets for cancers.

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“…1–3) We also found unique glycosphingolipids and biosynthetic pathways in animals bearing tumors and human tumor sources. 6–14) Through these studies, we demonstrated that some of these aberrant glycosphingolipids and metabolisms in tumor tissues were a kind of acquired mimetic glycosphingolipid expression arising during the tumorigenic process, which could be found in components of the intrinsic immune systems of host animals, such as macrophages and lymphocytes, to escape from the immune-surveillance system. 7,15–17) In glycosphingolipid studies performed in the 1980s, much attention was focused on tumor-associated antigens and monoclonal antibody (MAb) productions and their potential therapeutic application.…”

Section: Introductionmentioning

confidence: 97%

Bio-recognition and functional lipidomics by glycosphingolipid transfer technology

Taki

2013

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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Through glycosphingolipid biochemical research, we developed two types of transcription technologies. One is a biochemical transfer of glycosphingolipids to peptides. The other is a physicochemical transfer of glycosphingolipids in silica gel to the surface of a plastic membrane. Using the first technology, we could prepare peptides which mimic the shapes of glycosphingolipid molecules by biopanning with a phage-displayed peptide library and anti-glycosphingolipid antibodies as templates. The peptides thus obtained showed biological properties and functions similar to those of the original glycosphingolipids, such as lectin binding, glycosidase modulation, inhibition of tumor metastasis and immune response against the original antigen glycosphingolipid, and we named them glyco-replica peptides. The results showed that the newly prepared peptides could be used effectively as a bio-recognition system and suggest that the glyco-replica peptides can be widely applied to therapeutic fields. Using the second technology, we could establish a functional lipidomics with a thin-layer chromatography-blot/matrix-assisted laser desorption ionization-time of flight mass spectrometry (TLC-Blot/MALDI-TOF MS) system. By transferring glycosphingolipids on a plastic membrane surface from a TLC plate, innovative biochemical approaches such as simple purification of individual glycosphingolipids, binding studies, and enzyme reactions could be developed. The combinations of these biochemical approaches and MALDI-TOF MS on the plastic membrane could provide new strategies for glycosphingolipid science and the field of lipidomics. In this review, typical applications of these two transfer technologies are introduced.

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Occurrence of monosialosyl pentahexaosylceramide GalNAc-GM1 as specific tumor-associated ganglioside of human head and neck squamous cell carcinomas

Cited by 7 publications

References 15 publications

Expressão de glicoesfingolipídeos no carcinoma espinocelular do trato aerodigestivo superior

Expressão de glicoesfingolipídeos no carcinoma espinocelular do trato aerodigestivo superior

Glycomic Analysis of Cancer

Bio-recognition and functional lipidomics by glycosphingolipid transfer technology

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