Occurrence of Metallo-β-lactamase Genes among Acinetobacter baumannii Isolated from Different Clinical Samples

Ridha, Dunya Jawad; Ali, Munim Radwan; Jassim, Kifah Ahmed

doi:10.22207/jpam.13.2.50

Cited by 7 publications

(13 citation statements)

References 18 publications

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“…Biofilm formation and regulatory genes have been investigated as controversial and critical issues in healthcare settings. However, in some studies, no apparent relationship between β-lactamase enzymes and biofilm formation has been detected 21,22 . Further, as shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…By comparing the results of various studies we determine a significant relationship between β-lactamase enzymes and biofilm operons in A. baumannii 24,27 . However, some researcher showed that there is no significant relationship between biofilm formation and β-lactamase enzymes in P. aeruginosa 22 . This is in contrast to our results, which confirm that the biofilm regulatory gene in P. aeruginosa is most abundant in the β-lactamase producing strains (~35% in resistant vs. ~11% in susceptible, p< 0.001).…”

Section: Discussionmentioning

confidence: 99%

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Relationship between Biofilm Regulating Operons and Various Β-Lactamase Enzymes: Analysis of the Clinical Features of Infections caused by Non-Fermentative Gram-Negative Bacilli (Nfgnb) from Iran

Porbaran¹,

Habibipour²

2020

J Pure Appl Microbiol

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Bacteria are capable of evolving high doses of the drug in various infections by forming biofilms. Perhaps, biofilm regulator genes have different frequencies in β-lactam producing non-fermentative Gram-negative Bacilli (NFGNB). In this study, we investigated the role of biofilm operons of Pseudomonas aeruginosa and Acinetobacter baumannii on the prevalence of different β-lactamase enzymes. One-hundred twenty (120) nosocomial NFGNB isolates were collected from different clinical samples of patients. PCR method was used for the amplification of resistance genes. Isolates were collected, including 50 isolates (41.66%) of P. aeruginosa and 70 isolates (58.33%) of A. baumannii. The distribution of ESBL, AmpC, KPC, and MBL β-lactamase enzymes in P. aeruginosa and A. baumannii isolates were 64%, 58%, 38%,44%, and 57.14%, 60%, 32.85%, 34.28%, respectively. The frequency of csuABC, pgaABC operon in A. baumannii were as follows: pgaA (45.71%), pgaB (32.85%), pgaC (42.85%), csuA (34.28%), csuB (32.85%), csuC (41.42%), and ompA (38.57%). Further, the prevalence of pslABC and pelABC operons in P. aeruginosa isolates were as follows: pslA (58%), pslB (58%), pslD (60%), pelA (64%), pelB (38%), pelC (44%), and algD (68%). This study revealed that the abundance of biofilm regulator genes in NFGNB strains is affected by different β-lactamase enzymes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Relationship between Biofilm Regulating Operons and Various Β-Lactamase Enzymes: Analysis of the Clinical Features of Infections caused by Non-Fermentative Gram-Negative Bacilli (Nfgnb) from Iran

Porbaran¹,

Habibipour²

2020

J Pure Appl Microbiol

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“…Additionally, the majority of A. baumannii 99% were resistant to ciprofloxacin, 98% were resistance to ceftazidime, meropenem, and imipenem, 77% were not sensitive to gentamicin, and 48% were resistance to amikacin 36 . Our results showed that most of A. baumannii isolates 98.5% were highly susceptible to colistin, as it mainly targets the LPS components, and attenuates the colonization of A. baumannii in lung epithelial cells 37 .…”

Section: Discussionmentioning

confidence: 68%

Evaluating the Activity of Ultrasound on Biofilm Formation by Acinetobacter baumannii isolated from clinical Specimens

2022

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Acinetobacter baumannii received attention for its multi-drug resistant associated with many severe infections and outbreaks in clinical environment. The aims of the study are to investigate the antibiotic susceptibility profile of clinically isolated A. baumannii, biofilm production, and the efficiency of Low Frequency Ultrasound (LFU) and honey to attenuate biofilm production. A total of 100 samples were taken from different sources from Baghdad hospitals. The susceptibility patterns revealed the percentage of pan drug resistant (PDR) isolates were 1.5 %, 72.7 % were extended drug resistant (XDR), 16.7 % were multidrug resistant (MDR), and 9.1 % were non MDR and sensitive to most antibiotics used. The ability to form biofilm was detected by crystal violet staining, and the results showed that 20% were strong biofilm, and 31.8% were moderate biofilm. The biofilm formation percentage was decreased using Low frequency ultrasound LFU and honey. Moreover, PCR results revealed that not all of them harbouring biofilm-related genes or integrons (bap, csuE, IntI-1, IntI-2), although, they are strong biofilm producers. These results conclude that low frequency ultrasound and chemical components of honey might be a good choice to restrain A. baumannii biofilm formation, and negative correlation between antibiotic resistance and biofilm ability. Running title: Antimicrobial drug resistance, Acinetobacter baumannii, biofilm inhibition

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“…Outer-membrane proteins: (OmpA, Omp 22) Adherence and invasion, apoptosis induction, biofilm formation, persistence, and serum resistance. [17,18] Lipopolysaccharide (LPS) Serum resistance, survival during tissue infection, evasion of immune system response. [18,19] Micronutrient Acquisition System (iron, manganese and zinc) Competition and survival cause the death of host cells.…”

Section: Virulence Factor Activity In Pathogenesis Referencementioning

confidence: 99%

Acinetobacter baumannii, a Multidrug-Resistant Opportunistic Pathogen in New Habitats: A Systematic Review

Ahuatzin-Flores,

Torres,

Chávez-Bravo

2024

Microorganisms

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In recent years, humanity has begun to face a growing challenge posed by a rise in the prevalence of antibiotic-resistant bacteria. This has resulted in an alarming surge in fatalities and the emergence of increasingly hard-to-manage diseases. Acinetobacter baumannii can be seen as one of these resilient pathogens due to its increasing prevalence in hospitals, its resistance to treatment, and its association with elevated mortality rates. Despite its clinical significance, the scientific understanding of this pathogen in non-hospital settings remains limited. Knowledge of its virulence factors is also lacking. Therefore, in this review, we seek to shed light on the latest research regarding the ecological niches, microbiological traits, and antibiotic resistance profiles of Acinetobacter baumannii. Recent studies have revealed the presence of this bacterium in a growing range of environmental niches, including rivers, treatment plants, and soils. It has also been discovered in diverse food sources such as meat and vegetables, as well as in farm animals and household pets such as dogs and cats. This broader presence of Acinetobacter baumannii, i.e., outside of hospital environments, indicates a significant risk of environmental contamination. As a result, greater levels of awareness and new preventive measures should be promoted to address this potential threat to public health.

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Occurrence of Metallo-β-lactamase Genes among Acinetobacter baumannii Isolated from Different Clinical Samples

Cited by 7 publications

References 18 publications

Relationship between Biofilm Regulating Operons and Various Β-Lactamase Enzymes: Analysis of the Clinical Features of Infections caused by Non-Fermentative Gram-Negative Bacilli (Nfgnb) from Iran

Relationship between Biofilm Regulating Operons and Various Β-Lactamase Enzymes: Analysis of the Clinical Features of Infections caused by Non-Fermentative Gram-Negative Bacilli (Nfgnb) from Iran

Evaluating the Activity of Ultrasound on Biofilm Formation by Acinetobacter baumannii isolated from clinical Specimens

Acinetobacter baumannii, a Multidrug-Resistant Opportunistic Pathogen in New Habitats: A Systematic Review

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